Luminal calcium concentration controls intestinal calcium absorption by modification of intestinal alkaline phosphatase activity

Brun, L.R.; Brance, María Lorena; Rigalli, Alfredo

doi:10.1017/s0007114511005617

Cited by 27 publications

(29 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Dietary added calcium stimulates IAP in rat intestine [39]. Calcium is known to be protective in colonic inflammation models but the implication of IAP was not explored.…”

Section: Introductionmentioning

confidence: 99%

Microbiota-host interplay at the gut epithelial level, health and nutrition

Lallès

2016

J Animal Sci Biotechnol

View full text Add to dashboard Cite

Growing evidence suggests the implication of the gut microbiota in various facets of health and disease. In this review, the focus is put on microbiota-host molecular cross-talk at the gut epithelial level with special emphasis on two defense systems: intestinal alkaline phosphatase (IAP) and inducible heat shock proteins (iHSPs). Both IAP and iHSPs are induced by various microbial structural components (e.g. lipopolysaccharide, flagellin, CpG DNA motifs), metabolites (e.g. n-butyrate) or secreted signal molecules (e.g., toxins, various peptides, polyphosphate). IAP is produced in the small intestine and secreted into the lumen and in the interior milieu. It detoxifies microbial components by dephosphorylation and, therefore, down-regulates microbe-induced inflammation mainly by inhibiting NF-κB pro-inflammatory pathway in enterocytes. IAP gene expression and enzyme activity are influenced by the gut microbiota. Conversely, IAP controls gut microbiota composition both directly, and indirectly though the detoxification of pro-inflammatory free luminal adenosine triphosphate and inflammation inhibition. Inducible HSPs are expressed by gut epithelial cells in proportion to the microbial load along the gastro-intestinal tract. They are also induced by various microbial components, metabolites and secreted molecules. Whether iHSPs contribute to shape the gut microbiota is presently unknown. Both systems display strong anti-inflammatory and anti-oxidant properties that are protective to the gut and the host. Importantly, epithelial gene expressions and protein concentrations of IAP and iHSPs can be stimulated by probiotics, prebiotics and a large variety of dietary components, including macronutrients (protein and amino acids, especially L-glutamine, fat, fiber), and specific minerals (e.g. calcium) and vitamins (e.g. vitamins K1 and K2). Some food components (e.g. lectins, soybean proteins, various polyphenols) may inhibit or disturb these systems. The general cellular and molecular mechanisms involved in the microbiota-host epithelial crosstalk and subsequent gut protection through IAP and iHSPs are reviewed along with their nutritional modulation. Special emphasis is also given to the pig, an economically important species and valuable biomedical model.

show abstract

“…Dietary added calcium stimulates IAP in rat intestine [39]. Calcium is known to be protective in colonic inflammation models but the implication of IAP was not explored.…”

Section: Introductionmentioning

confidence: 99%

Microbiota-host interplay at the gut epithelial level, health and nutrition

Lallès

2016

J Animal Sci Biotechnol

View full text Add to dashboard Cite

show abstract

“…Thus Alpi is located at the interface between the intestinal tissue, the ingesta and the vast microbiota, which suggests its involvement in a variety of biological processes. Recent studies have discovered that Alpi regulates metabolism by controlling the uptake of nutrients such as lipids (Narisawa et al, 2003; Lynes et al, 2011) and calcium (Brun et al, 2012), affects gut physiology by maintaining protective surface microclimate pH in the duodenum (Akiba et al, 2007; Mizumori et al, 2009), and impacts innate immunity by modulating bacterial LPS-induced inflammation (Poelstra et al, 1997b; Bates et al, 2007; Campbell et al, 2010). …”

Section: Introductionmentioning

confidence: 99%

Dynamic Evolution of the LPS-Detoxifying Enzyme Intestinal Alkaline Phosphatase in Zebrafish and Other Vertebrates

et al. 2012

View full text Add to dashboard Cite

Alkaline phosphatases (Alps) are well-studied enzymes that remove phosphates from a variety of substrates. Alps function in diverse biological processes, including modulating host-bacterial interactions by dephosphorylating the Gram-negative bacterial cell wall component lipopolysaccharide (LPS). In animals, Alps are encoded by multiple genes characterized by either ubiquitous expression (named Alpls for their liver expression, but a key to proper bone mineralization), or their tissue-specific expression, for example in the intestine (Alpi). We previously characterized a zebrafish alpi gene (renamed here alpi.1) that is regulated by Myd88-dependent innate immune signaling and that is required to prevent a host’s excessive inflammatory reactions to its resident microbiota. Here we report the characterization of two new alp genes in zebrafish, alpi.2 and alp3. To understand their origins, we investigated the phylogenetic history of Alp genes in animals. We find that vertebrate Alp genes are organized in three clades with one of these clades missing from the mammals. We present evidence that these three clades originated during the two vertebrate genome duplications. We show that alpl is ubiquitously expressed in zebrafish, as it is in mammals, whereas the other three alps are specific to the intestine. Our phylogenetic analysis reveals that in contrast to Alpl, which has been stably maintained as a single gene throughout the vertebrates, the Alpis have been lost and duplicated multiple times independently in vertebrate lineages, likely reflecting the rapid and dynamic evolution of vertebrate gut morphologies, driven by changes in bacterial associations and diet.

show abstract

“…In humans, net Ca absorption is not higher than 500–600 mg/day, even when Ca intake goes above 1000 mg/day [2]. A similar situation has been observed in rats [3], and a minute-to-minute regulatory mechanism of Ca uptake dependent of Ca luminal concentration by intestinal alkaline phosphatase (IAP) activity has been proposed [4]. …”

Section: Introductionmentioning

confidence: 85%

Increased calcium uptake and improved trabecular bone properties in intestinal alkaline phosphatase knockout mice

et al. 2017

Self Cite

View full text Add to dashboard Cite

Previous studies have demonstrated a negative correlation between intestinal alkaline phosphatase (IAP) activity and calcium (Ca) absorption in the gut, as IAP acts as a protective mechanism inhibiting high Ca entry into enterocytes, preventing Ca overload. Here we evaluated Ca absorption and bone properties in knockout mice (KO) completely devoid of duodenal IAP (Akp3 mice). Female C57BL/6 control mice (WT, n = 7) and KO mice (n = 10) were used to determine Ca absorption in vivo and by in situ isolated duodenal loops followed by histomorphometric analysis of duodenal villi and crypts. Bone mineral density, morphometry, histomorphometry and trabecular connectivity and biomechanical properties were measured on bones. We observed mild atrophy of the villi with lower absorption surface and a significantly higher Ca uptake in KO mice. While no changes were seen in cortical bone, we found better trabecular connectivity and biomechanical properties in the femurs of KO mice compared to WT mice. Our data indicate that IAP KO mice display higher intestinal Ca uptake, which over time appears to correlate with a positive effect on the biomechanical properties of trabecular bone.

show abstract

Luminal calcium concentration controls intestinal calcium absorption by modification of intestinal alkaline phosphatase activity

Cited by 27 publications

References 22 publications

Microbiota-host interplay at the gut epithelial level, health and nutrition

Microbiota-host interplay at the gut epithelial level, health and nutrition

Dynamic Evolution of the LPS-Detoxifying Enzyme Intestinal Alkaline Phosphatase in Zebrafish and Other Vertebrates

Increased calcium uptake and improved trabecular bone properties in intestinal alkaline phosphatase knockout mice

Contact Info

Product

Resources

About