“…After sacrifice with euthanyl(3.0-4.0 mlkg, 240 mg pentobarbitol, and 0.20 ml propylene glycol per ml) (MTC Pharmaceuticals, Cambridge, Ontario), the aorta was fixed in neutral buffered formalin and embedded in paraffin for sectioning as has been previously described [26,271. Sections were stained with hematoxylin and eosin for analysis of intimal hyperplasia and atherosclerotic plaque development after laser ablation.…”
“…This model has been used and developed in our laboratory for investigation into restenosis as well as plaque development [26,27]. Under general inhalation anesthetic, Hallothane titrated to effect and administered through a hood attachment designed for use in roosters, a 5 French Cook sheath was advanced retrograde into the femoral artery via cut down.…”
“…After sacrifice with euthanyl(3.0-4.0 mlkg, 240 mg pentobarbitol, and 0.20 ml propylene glycol per ml) (MTC Pharmaceuticals, Cambridge, Ontario), the aorta was fixed in neutral buffered formalin and embedded in paraffin for sectioning as has been previously described [26,271. Sections were stained with hematoxylin and eosin for analysis of intimal hyperplasia and atherosclerotic plaque development after laser ablation.…”
“…This model has been used and developed in our laboratory for investigation into restenosis as well as plaque development [26,27]. Under general inhalation anesthetic, Hallothane titrated to effect and administered through a hood attachment designed for use in roosters, a 5 French Cook sheath was advanced retrograde into the femoral artery via cut down.…”
(1) Fluorescence spectra recorded from dog aorta were significantly altered by injection of collagens I and III, cholesterol, and elastin. (2) LF may allow quantitative assessment of plaque chemical content.
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