&lt;p&gt;Prevalence and antimicrobial resistance of &lt;em&gt;Shigella&lt;/em&gt; species isolated from diarrheal patients in Ahvaz, southwest Iran&lt;/p&gt;

Sheikh, Ahmad Farajzadeh; Moosavian, Mojtaba; Abdi, Mahtab; Heidary, Mohsen; Shahi, Fatemeh; Jomehzadeh, Nabi; Seyed‐Mohammadi, Sakineh; Saki, Morteza; Khoshnood, Saeed

doi:10.2147/idr.s187861

Cited by 32 publications

(21 citation statements)

References 16 publications

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“…Several studies were conducted to characterize bacteria associated with gastro-intestinal infection and found Shigella sp as one of the bacteria associated with diarrhea. The inding of this study was in conformity with that of Elmanama and Abdelateef [24] in Palestine and Sheikh, et al [25] in Iran who both found Shigella in stool sample of Children diagnosed with diarrhea as 16.7% and 13.2% respectively. Similar study was conducted by Obi, et al [26] on enteric bacterial pathogen in stools of residents of urban and rural regions of Nigeria, the results shows the most frequently encountered pathogens in rural area are E. coli, followed by Salmonella, Shigella, Enterobacter and Campylobacter.…”

Section: Discussionsupporting

confidence: 91%

Determination of antibiotics susceptibility profile of Shigella species isolated from children with acute diarrhea

Ali¹,

Diso²,

Abdullah³

et al. 2020

Int J Clin Microbiol Biochem Technol

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Diarrheal diseases continue to be the major cause of morbidity and mortality among children under 5 years. This study aimed to isolate, identify and determining the prevalence, antimicrobial susceptibility profile of Shigella sp associated with acute diarrhea among children in Kano, Northern Nigeria. A cross sectional study was conducted among children less than 5 years diagnosed with acute diarrhea and admitted to paediatric ward of Murtala Muhammad Specialist Hospital Kano. Stool samples from a total of 37 (20 male and 17 female) subjects were used to isolate and identified the pathogen. Antimicrobial susceptibility test was conducted using disc diffusion method. The result showed 12 out of 37 samples were positive for Shigella sp which accounted for 32.4%. Higher incidence of Shigella sp was found among subjects of age between 2 – 3 years. The isolates were 100% resistant to Ampicillin. High resistance was also observed in Amoxicillin (83.33%), Chloramphenicol (58.33%) and Tetracycline (25%). The isolates are 100% sensitive to ciprofloxacin, 66.7% to Levofloxacin and Gentamicin each and 58.33% to Erythromycin. Three (3) isolates were resistance to Ampicillin and Amoxicillin, 5 isolates were resistance to Ampicillin, Chloramphenicol and Amoxicillin while 2 isolates were resistance to Ampicillin, Chloramphenicol, Tetracycline and Amoxicillin. It is concluded that Shigella sp is one of the etiological agent of diarrhea in children. Ciprofloxacin, levofloxacin and Gentamicin are drugs of choice for treating diarrhea caused by Shigella sp.

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Section: Discussionsupporting

confidence: 91%

Determination of antibiotics susceptibility profile of Shigella species isolated from children with acute diarrhea

Ali¹,

Diso²,

Abdullah³

et al. 2020

Int J Clin Microbiol Biochem Technol

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“…The total DNA was extracted from fresh colonies of Enterobacteriaceae isolates by the boiling method as described previously. 12 Briefly, 2 or 3 colonies of an overnight growth of each isolate on nutrient agar (Merck Co., Darmstadt, Germany) were suspended in 500 mL of DNase-and RNasefree water. The suspension was boiled at 100°C for 10 min in a dry block incubator (Polystat 5; Bioblock Scientific, France), then centrifuged at 14,000 g for 10 min.…”

Section: Dna Extractionmentioning

confidence: 99%

<p>Prevalence of Extended-Spectrum Beta-Lactamase-Producing <em>Enterobacteriaceae</em> Causing Bloodstream Infections in Cancer Patients from Southwest of Iran</p>

Montazeri

Khosravi

Saki

et al. 2020

IDR

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This study aimed to evaluate the frequency rate of extended-spectrum betalactamase-producing Enterobacteriaceae (ESBL-PE) causing bloodstream infections (BSIs) in cancer patients referred to one of the major referral hospitals in Ahvaz city, southwest Iran. Materials and Methods: In this study, 1700 blood cultures were collected from 610 cancer patients suspected to have BSI from October 2016 to August 2017 referred to the Shafa cancer hospital, Ahvaz, southwest of Iran. The blood culture bottles were incubated aerobically at 35-37ºC for 24 hours and then sub-cultured on routine microbiology culture media. The bacterial colonies were identified using standard tests. The antibiotic susceptibility testing was achieved by the disc-diffusion method. The phenotypic detection of ESBLs was carried out by the combination disc-diffusion test (CDDT). Finally, the polymerase chain reaction (PCR) was performed to investigate the presence of bla TEM , bla CTX , bla SHV, and bla PER genes. Results: The prevalence of BSI in cancer patients was 16.4% (100/610). Gram-negative rods with rate of 74% (74/100) were the most prevalent bacteria. The frequency of Enterobacteriaceae family was 21% including Escherichia coli (n: 8), Klebsiella pneumoniae (n: 6), Enterobacter spp. (n: 5), Citrobacter freundii (n: 1), and Serratia marcescens (n: 1). All isolates were multidrug-resistant (resistance to three or more antibiotics). The results of CDDT showed that 42.8% (9/21) of Enterobacteriaceae isolates had a positive ESBL test of which 100% (9/9) indicated positive band for at least one of the ESBL genes by PCR method. The bla CTX-M and bla TEM genes were detected in 38% (8/21) and 23.8% (5/21) of isolates, respectively, while the bla SHV and bla PER were not detected in any isolates. Conclusion: Based on the results, surveillance, and antibiotic stewardship programs should be implemented for cancer patients to prevent the spread of more ESBL-PE that have limited therapeutically choices.

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“…Despite higher sensitivity and speci city, this method is very expensive so many researchers have made efforts to develop new alternatives. Because of high-cost, complicated, and sensitive production process of monoclonal antibodies in serology-based methods, molecular assays as well as DNA based techniques are preferred to be developed for species identi cation of bacterial pathogens [15]. However, identi cation of S. dysenteriae because of harboring shig-toxin encoded gene as an individual marker of this pathogen is not regarded as a real challenge in comparison with identi cation of other species [16].…”

Section: Resultsmentioning

confidence: 99%

“…Biochemically con rmed colonies were further serologically analyzed for Shigella species identi cation. Serological identi cation was carried out using slide-agglutination test with DIFCO Shigella species speci c antisera (Difco Lab, Michigan, USA) as the gold standard assay [15].…”

Section: Shigella Isolation and Species Identi Cationmentioning

confidence: 99%

RAPD and ERIC-PCR coupled with HRM for species identification of non-dysenteriae Shigella species isolated from clinical specimens; As a potential of alternative method

Pakbin

Basti

Khanjari

et al. 2020

Preprint

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Background: Shigella species cause gastrointestinal disorders and diarrhea in adults and children, also known as fatal and invasive pathogens for infants and neonates. Species identification of Shigella isolates are so prominent for epidemiological studies and implementing infection prevention strategies. At the present study, we developed and evaluated RAPD and ERIC-PCR assays coupled with HRM for differentiation of non-dysenteriae Shigella species as potential alternative methods. isolation of eighteen Shigella strains from faecal specimens collected from children under 2 years old with diarrhea symptom (n=143), species of the isolates were identified by slide agglutination assay as the golden standard method. Species of isolates were identified using developed RAPD-PCR-HRM and ERIC-PCR-HRM assays and differentiation of data sets obtained from difference plots of HRM was implemented by PCA as a dimension reduction method then sensitivity and specificity of the methods were evaluated using ROC curve. Results: Consequently, we found that RAPD-PCR-HRM with sensitivity and specificity of 100 and 85% respectively is significantly more suitable for identification of non-dysenteriae Shigella species. However, sensitivity and specificity of ERIC-PCR-HRM were evaluated 33 and 46% respectively.Conclusion: Regardless of inherent poor reproducibility of DNA fingerprinting based methods, RAPD-PCR-HRM assay can be considered as a potential of alternative method for species identification. Also, HRM technique used in this study is more rapid, inexpensive, and sensitive than gel electrophoresis for characterization of PCR amplicons.

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<p>Prevalence and antimicrobial resistance of <em>Shigella</em> species isolated from diarrheal patients in Ahvaz, southwest Iran</p>

Cited by 32 publications

References 16 publications

Determination of antibiotics susceptibility profile of Shigella species isolated from children with acute diarrhea

Determination of antibiotics susceptibility profile of Shigella species isolated from children with acute diarrhea

<p>Prevalence of Extended-Spectrum Beta-Lactamase-Producing <em>Enterobacteriaceae</em> Causing Bloodstream Infections in Cancer Patients from Southwest of Iran</p>

RAPD and ERIC-PCR coupled with HRM for species identification of non-dysenteriae Shigella species isolated from clinical specimens; As a potential of alternative method

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