&lt;i&gt;Ulva partita&lt;/i&gt; sp. nov., a Novel &lt;i&gt;Enteromorpha&lt;/i&gt;-Like &lt;i&gt;Ulva&lt;/i&gt; Species from Japanese Coastal Areas

Ichihara, Kensuke; Suzuki, Ritsu; Yamazaki, T.; Ota, Shuhei; Mogi, Yuko; Kagami, Yayoi; Kuwano, Kazuyoshi; Kawano, Shigeyuki

doi:10.1508/cytologia.80.261

Cited by 11 publications

(6 citation statements)

References 36 publications

(48 reference statements)

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“…Comparison with other chloroplast genomes Ulva pertusa exhibits a phenotype of lettuce-like morphology similar to the morphology of U. ohnoi and U. lactuca. However, evolutionary studies on these algae have suggested that U. pertusa is closely related to U. flexuosa and U. linza, and this species has been classified into a clade separate from that containing U. ohnoi and U. lactuca (Kraft et al, 2010;Ichihara et al, 2015;Matsumoto and Shimada, 2015). Using the complete chloroplast genome sequences, we generated a phylogenetic tree of Ulva species, including U. pertusa.…”

Section: Resultsmentioning

confidence: 99%

Construction of genomic marker sets based on the chloroplast genome of a green alga, Ulva pertusa (syn. Ulva australis), leads to simple detection of Ulva species

2020

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In closed sea areas such as Tokyo Bay, a phenomenon known as a green tide often occurs, in which large amounts of Ulva seaweed grow abnormally and form mats along the coast. This is currently a serious environmental problem. Green tides are generated by the explosive growth of multiple types of Ulva algae. However, many Ulva species show similar characteristics to each other and are indistinguishable by appearance, making it difficult to identify the Ulva algae in green tides. In this study, we determined the entire nucleotide sequence of the chloroplast genome of Ulva pertusa (syn. Ulva australis) and identified two large inversions of gene order, suggesting the occurrence of genome inversions. We also detected structural polymorphisms among Ulva chloroplast genomes. Ulva pertusa was classified in a different clade from that containing U. lactuca and U. ohnoi, suggesting that U. pertusa is evolutionarily divergent from these species. Based on this knowledge, we constructed a genetic diagnosis system for Ulva algae. Using this approach, we established a simple method that can determine the species of Ulva algae by PCR using specific molecular markers, through which representative Ulva species such as U. lactuca, U. ohnoi and U. pertusa were easily distinguished.

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Section: Resultsmentioning

confidence: 99%

Construction of genomic marker sets based on the chloroplast genome of a green alga, Ulva pertusa (syn. Ulva australis), leads to simple detection of Ulva species

2020

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“…The relation between the gamete types (type α and β) and the inheritance pattern of cp-DNA has been reported in several ulvophycean species and C. reinhardtii (Table 2). It is particularly interesting that cp-DNA is always transmit- (Ichihara et al 2015). ** Uniparental inheritance from mt + gametes.…”

Section: Resultsmentioning

confidence: 99%

Sex-Specific Cell Fusion Pattern of Isogametes in Marine Green Alga, Acetabularia caliculus (Ulvophyceae, Chlorophyta)

Miyamura

Nagumo

2016

CYTOLOGIA

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Summary Using field emission scanning electron microscopy (FE-SEM) and fluorescence microscopy, the respective relations between the arrangements of the gamete cell fusion site and the inheritance pattern of chloroplast DNA (cp-DNA) as a sex-specific marker were studied for the isogamous dasycladalean alga Acetabularia caliculus. The gamete had two flagella elongated from the cell anterior. One oval-shaped eyespot situated on the cell posterior was visualized using FE-SEM. When the gametes belonging to the opposite mating types were mixed together, the two gametes aligned side-by-side at their lateral side and fused to form a quadriflagellate planozygote. In the planozygote, the two flagella from the opposite mating type gametes lay side-by-side and two eyespots aligned on the same side of the cell, suggesting opposite positioning of the cell fusion sites between two parental gametes. To confirm whether the gamete fusion pattern observed in the planozygotes was a result of sex-specific fusion or not, the inheritance pattern of cp-DNA was observed using fluorescence microscopy after staining with DAPI concomitantly with the cell fusion pattern. When the eyespots were used as positional marker and were viewed from the surface that included the eyespots, the chloroplast of one parental gamete was found to contain cp-DNAs, although cp-DNAs were not detected in the chloroplast derived from another parental gamete. These results suggest that the gamete fusion pattern observed by FE-SEM was a result of sex-specific fusion.

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“…The pairs of mt − (MGEC-2) and mt + (MGEC-1) strains of Ulva partita used were collected from the coast of Japan (Supplementary Table 7 ) 23 – 25 , 52 . We recently renamed this species from Ulva compressa to U .…”

Section: Methodsmentioning

confidence: 99%

Genomic structure and evolution of the mating type locus in the green seaweed Ulva partita

Yamazaki

Ichihara

Suzuki

et al. 2017

Sci Rep

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The evolution of sex chromosomes and mating loci in organisms with UV systems of sex/mating type determination in haploid phases via genes on UV chromosomes is not well understood. We report the structure of the mating type (MT) locus and its evolutionary history in the green seaweed Ulva partita, which is a multicellular organism with an isomorphic haploid-diploid life cycle and mating type determination in the haploid phase. Comprehensive comparison of a total of 12.0 and 16.6 Gb of genomic next-generation sequencing data for mt− and mt+ strains identified highly rearranged MT loci of 1.0 and 1.5 Mb in size and containing 46 and 67 genes, respectively, including 23 gametologs. Molecular evolutionary analyses suggested that the MT loci diverged over a prolonged period in the individual mating types after their establishment in an ancestor. A gene encoding an RWP-RK domain-containing protein was found in the mt− MT locus but was not an ortholog of the chlorophycean mating type determination gene MID. Taken together, our results suggest that the genomic structure and its evolutionary history in the U. partita MT locus are similar to those on other UV chromosomes and that the MT locus genes are quite different from those of Chlorophyceae.

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<i>Ulva partita</i> sp. nov., a Novel <i>Enteromorpha</i>-Like <i>Ulva</i> Species from Japanese Coastal Areas

Cited by 11 publications

References 36 publications

Construction of genomic marker sets based on the chloroplast genome of a green alga, <i>Ulva pertusa</i> (syn. <i>Ulva australis</i>), leads to simple detection of <i>Ulva</i> species

Construction of genomic marker sets based on the chloroplast genome of a green alga, <i>Ulva pertusa</i> (syn. <i>Ulva australis</i>), leads to simple detection of <i>Ulva</i> species

Sex-Specific Cell Fusion Pattern of Isogametes in Marine Green Alga, <i>Acetabularia caliculus</i> (Ulvophyceae, Chlorophyta)

Genomic structure and evolution of the mating type locus in the green seaweed Ulva partita

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