&lt;i&gt;Drosophila&lt;/i&gt; Ogg1 is required to suppress 8-oxo-guanine accumulation following oxidative stress

Yasukawa, Takashi; Nakahara, Yasuyuki; Hirai, Jun; Inoue, Yoshihiro H.

doi:10.1266/ggs.90.11

Cited by 6 publications

(5 citation statements)

References 59 publications

(61 reference statements)

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“…The climbing assays, which used a fly's instinct of negative geotaxis, were carried out according to a previous protocol (Oka et al., ; Yasukawa, Nakahara, Hirai, & Inoue, ). Flies were gently tapped to the bottom of a plastic culture tube (100 mm in height and 15 mm in diameter), and the number of flies that climbed up to a marked point (50 mm height) after 6 seconds was counted.…”

Section: Methodsmentioning

confidence: 99%

Identification ofmiR-305, a microRNA that promotes aging, and its target mRNAs inDrosophila

et al. 2018

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MicroRNAs (miRNAs) are involved in the regulation of important biological processes. Here, we describe a novel Drosophila miRNAs involved in aging. We selected eight Drosophila miRNAs, displaying high homology with seed sequences of aging-related miRNAs characterized in other species, and investigated whether the over-expression of these miRNAs affected aging in Drosophila adult flies. The lifespan of adults over-expressing miR-305, a miRNA showing high homology with miR-239 in C. elegans, was significantly shorter. Conversely, a reduction in miR-305 expression led to a longer lifespan than that in control flies. miR-305 over-expression accelerated the impairment of locomotor activity and promoted the age-dependent accumulation of poly-ubiquitinated protein aggregates in the muscle, as flies aged. Thus, we show that the ectopic expression of miR-305 has a deleterious effect on aging in Drosophila. To identify the targets of miR-305, we performed RNA-Seq. We discovered several mRNAs encoding antimicrobial peptides and insulin-like peptides, whose expression changed in adults upon miR-305 over-expression. We further confirmed, by qRT-PCR, that miR-305 over-expression significantly decreases the mRNA levels of four antimicrobial peptides. As these mRNAs contain multiple sequences matching the seed sequence of miR-305, we speculate that a reduction in target mRNA levels, caused by ectopic miRNA expression, promotes aging.

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Section: Methodsmentioning

confidence: 99%

Identification ofmiR-305, a microRNA that promotes aging, and its target mRNAs inDrosophila

et al. 2018

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“…qRT-PCR analysis was performed to determine the expression level of lamin genes and Nups genes in adult flies with induced expression of each dsRNA with a bam-Gal4 driver as described (Yasukawa et al, 2015). Total RNA was extracted from adult flies using the TRIzol reagent (Invitrogen, Carlsbad, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

B-type nuclear lamin and the nuclear pore complex Nup107-160 influences maintenance of the spindle envelope required for cytokinesis inDrosophilamale meiosis

et al. 2016

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In higher eukaryotes, nuclear envelope (NE) disassembly allows chromatin to condense and spindle microtubules to access kinetochores. The nuclear lamina, which strengthens the NE, is composed of a polymer meshwork made of A- and B-type lamins. We found that the B-type lamin (Lam) is not fully disassembled and continues to localize along the spindle envelope structure during Drosophila male meiosis I, while the A-type lamin (LamC) is completely dispersed throughout the cytoplasm. Among the nuclear pore complex proteins, Nup107 co-localized with Lam during this meiotic division. Surprisingly, Lam depletion resulted in a higher frequency of cytokinesis failure in male meiosis. We also observed the similar meiotic phenotype in Nup107-depleted cells. Abnormal localization of Lam was found in the Nup-depleted cells at premeiotic and meiotic stages. The central spindle microtubules became abnormal and recruitment of a contractile ring component to the cleavage sites was disrupted in Lam-depleted cells and Nup107-depleted cells. Therefore, we speculate that both proteins are required for a reinforcement of the spindle envelope, which supports the formation of central spindle microtubules essential for cytokinesis in Drosophila male meiosis.

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“…Exposure to 2000 pM of EMB affected both cell cycle regulation and DNA repair pathways. Interestingly, most of the supporting DEGs in these pathways were found to be repressed, such as DNA excision repair protein ERCC-1 ( Ercc1 ), double-strand break repair protein MRE11 ( Mre11 ) and 8-oxoguanine DNA glycosylase ( Ogg1 ), albeit these genes were normally up-regulated in arthropods after exposure to genotoxic agents. − Suppression of DNA repair may be caused by different mechanisms. One possible explanation may be the unbalanced expression between the proliferating cell nuclear antigen ( Pcna ) and cyclin-dependent kinase inhibitor 1 ( P21 ) genes which are upstream regulators of DNA repair signaling. , Interestingly, the Pcna gene was down-regulated in a concentration-dependent manner, whereas the double-strand-break repair protein rad21 homologue ( Vtd ) gene were slightly up-regulated after exposure to 500 pM EMB and marginally down-regulated by exposure to 2000 pM EMB in this study, suggesting that suppression of DNA repair signaling may likely be a consequence of abnormal expression of these genes in D. magna after exposure to EMB.…”

Section: Resultsmentioning

confidence: 99%

“…Interestingly, most of the supporting DEGs in these pathways were found to be repressed, such as DNA excision repair protein ERCC-1 (Ercc1), double-strand break repair protein MRE11 (Mre11) and 8-oxoguanine DNA glycosylase (Ogg1), albeit these genes were normally up-regulated in arthropods after exposure to genotoxic agents. [54][55][56][57][58] Suppression of DNA repair may be caused by different mechanisms. One possible explanation may be the unbalanced expression between the proliferating cell nuclear antigen (PCNA) and cyclin-dependent kinase inhibitor 1 (P21) genes which are upstream regulators of DNA repair signaling.…”

Section: Toxic Mechanisms Activation Of Ligand-gated Chloride Channels In Line With the Previously Proposedmentioning

confidence: 99%

Whole-Organism Transcriptomic Analysis Provides Mechanistic Insight into the Acute Toxicity of Emamectin Benzoate in Daphnia magna

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Rundberget

Evenseth

et al. 2016

Environ. Sci. Technol.

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Emamectin benzoate (EMB) is an antisea lice chemical widely used in the aquaculture that may also unintentionally affect nontarget crustaceans in the environment. Although the adverse effects of this compound are well documented in various species, the full modes of action (MoAs) are still not well characterized. The current study was therefore conducted to characterize the MoAs of EMB and link perturbations of key toxicological pathways to adverse effects in the model freshwater crustacean Daphnia magna. Effects on molting and survival were determined after 48 h exposure to EMB, whereas global transcriptional changes and the ecdysone receptor (EcR) binding potency was determined to characterize the MoA. The results showed that the molting frequency and survival of D. magna decreased in a concentration-dependent manner, and the observed changes could not be attributed to direct interactions with the EcR. Major MoAs such as activation of glutamate-gated chloride channels and gamma-aminobutyric acid signaling, disruption of neuroendocrine regulation of molting, perturbation of energy homeostasis, suppression of DNA repair and induction of programmed cell death were observed by transcriptional analysis and successfully linked to the adverse effects. This study has demonstrated that acute exposure to intermediate and high pM levels of EMB may pose hazards to nontarget crustaceans in the aquatic environment.

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<i>Drosophila</i> Ogg1 is required to suppress 8-oxo-guanine accumulation following oxidative stress

Cited by 6 publications

References 59 publications

Identification ofmiR-305, a microRNA that promotes aging, and its target mRNAs inDrosophila

Identification ofmiR-305, a microRNA that promotes aging, and its target mRNAs inDrosophila

B-type nuclear lamin and the nuclear pore complex Nup107-160 influences maintenance of the spindle envelope required for cytokinesis inDrosophilamale meiosis

Whole-Organism Transcriptomic Analysis Provides Mechanistic Insight into the Acute Toxicity of Emamectin Benzoate in Daphnia magna

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