&lt;i&gt;Angelica keiskei&lt;/i&gt; Ameliorates Scopolamine-Induced Memory Impairments in Mice

Oh, Se Yeon; Kim, Su Jin; Kim, Dong Hyun; Ryu, Jong Hoon; Ahn, Eun Mi; Jung, Ji Wook

doi:10.1248/bpb.b12-00681

Cited by 27 publications

(18 citation statements)

References 31 publications

(29 reference statements)

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“…Thus, we examined the effects of HCW in Aβ-injected mice model. Then we continued to work on the study using scopolamine-injected mice, a cholinergic dysfunction model of an antagonist of ACh because scopolamine induce memory deficits are similar to those found in age-related dysfunction in central nervous system and is a useful tool for investigating learning and memory studies which involved in cholinergic system (Oh et al ., 2013). We performed the Y-maze task and PAT to estimate memory consolidation (Conrad et al ., 1996; Roozendaal, 2002).…”

Section: Discussionmentioning

confidence: 99%

Houttuynia cordata Improves Cognitive Deficits in Cholinergic Dysfunction Alzheimer’s Disease-Like Models

Huh

Kim

Park

et al. 2014

Biomolecules & Therapeutics

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Cognitive impairment is a result of dementia of diverse causes, such as cholinergic dysfunction and Alzheimer’s disease (AD). Houttuynia cordata Thunb. (Saururaceae) has long been used as a traditional herbal medicine. It has biological activities including protective effects against amyloid beta (Aβ) toxicity, via regulation of calcium homeostasis, in rat hippocampal cells. To extend previous reports, we investigated the effects of water extracts of H. cordata herb (HCW) on tauopathies, also involving calcium influx. We then confirmed the effects of HCW in improving memory impairment and neuronal damage in mice with Aβ-induced neurotoxicity. We also investigated the effects of HCW against scopolamine-induced cholinergic dysfunction in mice. In primary neuronal cells, HCW inhibited the phosphorylation of tau by regulating p25/p35 expression in Aβ-induced neurotoxicity. In mice with Aβ-induced neurotoxicity, HCW improved cognitive impairment, as assessed with behavioral tasks, such as novel object recognition, Y-maze, and passive avoidance tasks. HCW also inhibited the degeneration of neurons in the CA3 region of the hippocampus in Aβ-induced neurotoxicity. Moreover, HCW, which had an IC50 value of 79.7 μg/ml for acetylcholinesterase inhibition, ameliorated scopolamine-induced cognitive impairment significantly in Y-maze and passive avoidance tasks. These results indicate that HCW improved cognitive impairment, due to cholinergic dysfunction, with inhibitory effects against tauopathies and cholinergic antagonists, suggesting that HCW may be an interesting candidate to investigate for the treatment of AD.

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Section: Discussionmentioning

confidence: 99%

Houttuynia cordata Improves Cognitive Deficits in Cholinergic Dysfunction Alzheimer’s Disease-Like Models

Huh

Kim

Park

et al. 2014

Biomolecules & Therapeutics

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“…Western blot analysis was performed as described previously [23] with some modification. BV-2 cells were plated at a density of 1 × 10 6 cells per ml in a 6-well cell culture plate with 2 ml of culture medium and incubated for 24 h. The cells were pre-treated with FCL for 1 h and stimulated with LPS (100 ng/ml) for specified time periods.…”

Section: Methodsmentioning

confidence: 99%

The protective effect of fermented Curcuma longa L. on memory dysfunction in oxidative stress-induced C6 gliomal cells, proinflammatory-activated BV2 microglial cells, and scopolamine-induced amnesia model in mice

Eun¹,

Lim²,

Lee³

et al. 2017

BMC Complement Altern Med

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Background Curcuma longa L. is a well-known medicinal plant that has been used for its anti-cancer, neuroprotective, and hepatoprotective effects. However, the neuroprotective effect of fermented C. longa (FCL) has not been reported. Therefore, in this study, the effectiveness of FCL for the regulation of memory dysfunction was investigated in two brain cell lines (rat glioma C6 and murine microglia BV2) and scopolamine-treated mice.Methods C. longa powder was fermented by 5% Lactobacillus plantarum K154 containing 2% (w/v) yeast extract at 30 °C for 72 h followed by sterilization at 121 °C for 15 min. The protective effects of fermented C. longa (FCL) on oxidative stress induced cell death were analyzed by MTT assay in C6 cells. The anti-inflammatory effects of FCL were investigated by measuring the production of nitric oxide (NO) and prostaglandin E2 (PGE2) as well as the expression levels of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) in LPS-stimulated BV2 cells. The step-through passive avoidance test, Morris water maze test, acetylcholinesterase (AChE) activity, and expression of cAMP response element-binding protein (CREB) and brain-derived neurotropic factor (BDNF) were employed to determine the effects of FCL on scopolamine-induced memory deficit in mice. The contents of curcuminoids were analyzed through LC/MS.ResultsPretreatment with FCL effectively prevented the cell death induced by oxidative stress in C6 cells. Moreover, FCL inhibited the production NO and PGE2 via the inhibition of iNOS and COX-2 expression in BV2 cells. FCL significantly attenuated scopolamine-induced memory impairment in mice and prevented scopolamine-induced AChE activity in the hippocampus. Additionally, FCL reversed the reduction of CREB and BDNF expression. The curcuminoids content in FCL was 1.44%.ConclusionFCL pretreatment could alleviate scopolamine-induced memory impairment in mice, as well as oxidative stress and inflammation in C6 and BV2 cells, respectively. Thus, FCL might be a useful material for preventing impairment of learning and memory.

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“…5) We previously reported that the ethanolic extract of Angelica keiskei markedly ameliorated scopolamine-induced memory impairments via the inhibition of AChE activity as well as through the activation of cAMP response elementbinding protein (CREB)-brain-derived neurotrophic factor (BDNF) signaling. 6) Using activity-guided fractionation to identify the active compound(s), we isolated the active compounds α-and β-amyrin from the ethanolic extract of A. keiskei. Amyrin is a plant-derived pentacyclic triterpene and is present as either α-amyrin (vicinal methyls in the E ring) or β-amyrin (geminal methyls in the E ring) (Fig.…”

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confidence: 99%

Amyrin Attenuates Scopolamine-Induced Cognitive Impairment in Mice

Park

Ahn

et al. 2014

Biological & Pharmaceutical Bulletin

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Alzheimer's disease, a neurodegenerative disorder, is characterized by progressive cognitive impairment associated with the disruption of cholinergic neurotransmission. The aim of the present study was to evaluate the effect of α-or β-amyrin, a type of pentacyclic triterpene, on the cognitive impairment induced by scopolamine, a muscarinic acetylcholine receptor antagonist. To measure the abilities of various types of learning and memory, we conducted step-through passive avoidance task. Scopolamine induced deficits in learning and memory processes in mice, which were antagonized by a single administration of α-amyrin (2 or 4 mg/kg) or β-amyrin (4 mg/kg), respectively. Additionally, in vitro analysis revealed that acetylcholinesterase activity was inhibited by β-amyrin, but not by α-amyrin. Furthermore, Western blot analysis revealed that the expression levels of phosphorylated extracellular signal-regulated kinase 1/2 (pERK) and phosphorylated glycogen synthase kinase-3β (pGSK-3β) were significantly enhanced by a single administration of α-and β-amyrin in the hippocampus. Finally, the memory ameliorating effects of α-or β-amyrin on the scopolamine-induced cognitive impairments were significantly blocked by ERK inhibitor U0126. The present study suggests that α-and β-amyrin may ameliorate the cognitive impairment induced by hypocholinergic neurotransmission via the activation of ERK as well as GSK-3β signaling.

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<i>Angelica keiskei</i> Ameliorates Scopolamine-Induced Memory Impairments in Mice

Cited by 27 publications

References 31 publications

Houttuynia cordata Improves Cognitive Deficits in Cholinergic Dysfunction Alzheimer’s Disease-Like Models

Houttuynia cordata Improves Cognitive Deficits in Cholinergic Dysfunction Alzheimer’s Disease-Like Models

The protective effect of fermented Curcuma longa L. on memory dysfunction in oxidative stress-induced C6 gliomal cells, proinflammatory-activated BV2 microglial cells, and scopolamine-induced amnesia model in mice

Amyrin Attenuates Scopolamine-Induced Cognitive Impairment in Mice

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