&lt;em&gt;En Face&lt;/em&gt; Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries

Yu, Yi; Xiong, Yuyan; Montani, Jean‐Pierre; Yang, Zhihong; Ming, Xiu‐Fen

doi:10.3791/53718

Cited by 6 publications

(5 citation statements)

References 23 publications

(20 reference statements)

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“…In this sense, the normalization of superoxide content in cardiovascular tissues from obese Zucker rats by onion and apple supplementation could explain the improvement in endothelial vasodilation by these treatments ( Figure 1 ) and the absence of further potentiation of endothelial relaxations by the acute exposure to the superoxide scavenger or the NOX inhibitor in vessels from supplemented animals ( Figure 2 ). Elevated superoxide content has been detected in arteries from obese mice associated with decreased NO content [ 122 ]. Furthermore, in obese humans with insulin resistance, the increase in superoxide content in mesenteric microvasculature correlated with a reduction in endothelial vasodilation [ 110 ].…”

Section: Resultsmentioning

confidence: 99%

Onion and Apple Functional Ingredients Intake Improves Antioxidant and Inflammatory Status and Vascular Injury in Obese Zucker Rats

et al. 2022

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The objective of this study was to investigate the effects of onion and apple functional ingredients in homozygous (fa/fa) obese Zucker rats. Rodents were fed three diets: standard diet [obese control (OC) group], standard diet containing 10% onion [obese onion 10% (OO) group] and standard diet containing 10% apple [obese apple 10% (OA) group] for 8 weeks. Food intake and body weight gain were higher in obese than in lean rats. Food efficiency was lower in OO and AO groups compared with OC group. Within the obese groups, total cholesterol, LDL-cholesterol, triacylglycerols, glucose, insulin and triglyceride-glucose index were lower in OO group than in OC group, and HDL-cholesterol was higher in OO group than in OC group. In general, antioxidant activity (ABTS•+ and FRAP), antioxidant enzyme activities (CAT, SOD, GPx), GSH/GSSG ratio, nitrate/nitrite and GLP-1 increased in OO and OA groups compared with OC. Oxidative stress biomarkers, namely protein carbonyls, 8-hydroxy-2’-deoxyguanosine, 8-epi-prostaglandin F2α, inflammatory and vascular injury biomarkers (PAI-1, TIMP-1, VEGF, sICAM-1, sE-Selectin, MCP-1) and leptin, were lower in OO and OA groups than in OC group. Endothelial impairment was partially reversed, and superoxide content and gene expression of NLRP3, NFKβ1 and COX2 decreased, in OO and OA groups with respect to OC group. The study demonstrates that high pressure-processed onion and apple functional ingredients administration to obese Zucker rats causes beneficial effects on metabolic health, in particular through improving food efficiency ratio; exerting pronounced lipid-lowering effects; reducing glycemia, insulinemia, and biomarkers of hepatic injury (ALT, AST); improving antioxidant, oxidative stress, inflammatory and vascular injury biomarkers, metabolic hormones, and endothelial function; and decreasing proinflammatory gene expression of NLRP3, NFKβ1 and COX2.

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Section: Resultsmentioning

confidence: 99%

Onion and Apple Functional Ingredients Intake Improves Antioxidant and Inflammatory Status and Vascular Injury in Obese Zucker Rats

et al. 2022

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“…Cryostat transverse cuts (5 μm) of lung sections were freshly prepared at -20°C. For determination of NO bioavailability using 4-Amino-5-Methylamino-2',7'-Difluorofluorescein Diacetate (DAF-FM DA) fluorescent probe as a recognized method detecting intracellular levels of NO [ 38 ], lung tissue sections were incubated with 20 μM DAF-FM DA (Thermo Fisher Scientific, Cat# D23844, Houston, TX, USA) for 20 min in dark at 37°C. After being washed for 3 times, the sections were coverslipped.…”

Section: Methodsmentioning

confidence: 99%

Novel and robust treatment of pulmonary hypertension with netrin-1 and netrin-1-derived small peptides

Murugesan¹,

Zhang²,

Youn³

et al. 2022

Redox Biology

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“…Superoxide anoin and NO production was assessed with DHE and DAF-2DA staining as described previously Yu et al (2016 ). Briefly, old female mice aortas cleaned of perivascular tissues were equilibrated for 30 min in Krebs buffer at 37°C aerated with 95% O 2 and 5% CO 2 .…”

Section: Methodsmentioning

confidence: 99%

Detrimental Effects of Chronic L-Arginine Rich Food on Aging Kidney

Huang

Ladeiras

et al. 2021

Front. Pharmacol.

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The impaired L-arginine/nitric oxide pathway is a well-recognized mechanism for cardiovascular and renal diseases with aging. Therefore, supplementation of L-arginine is widely proposed to boost health or as adjunct therapy for the patients. However, clinical data, show adverse effects and even enhanced mortality in patients receiving long-term L-arginine supplementation. The effects of long-term L-arginine supplementation on kidney aging and the underlying mechanisms remain elusive. Moreover, high protein and high amino acid diet has been thought detrimental for kidney. We therefore investigated effects of chronic dietary L-arginine supplementation on kidney aging. In both young (4 months) and old (18–24 months) mice, animals either receive standard chow containing 0.65% L-arginine or diet supplemented with L-arginine to 2.46% for 16 weeks. Inflammation and fibrosis markers and albuminuria are then analyzed. Age-associated increases in tnf-α, il-1β, and il-6, vcam-1, icam-1, mcp1, inos, and macrophage infiltration, collagen expression, and S6K1 activation are observed, which is not favorably affected, but rather further enhanced, by L-arginine supplementation. Importantly, L-arginine supplementation further enhances age-associated albuminuria and mortality particularly in females, accompanied by elevated renal arginase-II (Arg-II) levels. The enhanced albuminuria by L-arginine supplementation in aging is not protected in Arg-II−/− mice. In contrast, L-arginine supplementation increases ROS and decreases nitric oxide production in old mouse aortas, which is reduced in Arg-II−/− mice. The results do not support benefits of long-term L-arginine supplementation. It rather accelerates functional decline of kidney and vasculature in aging. Thus, the long-term dietary L-arginine supplementation should be avoided particularly in elderly population.

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<em>En Face</em> Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries

Cited by 6 publications

References 23 publications

Onion and Apple Functional Ingredients Intake Improves Antioxidant and Inflammatory Status and Vascular Injury in Obese Zucker Rats

Onion and Apple Functional Ingredients Intake Improves Antioxidant and Inflammatory Status and Vascular Injury in Obese Zucker Rats

Novel and robust treatment of pulmonary hypertension with netrin-1 and netrin-1-derived small peptides

Detrimental Effects of Chronic L-Arginine Rich Food on Aging Kidney

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