Lowest Detectable Protein Immobilization Measurement Using an Ultrasensitive Micropillar-Based Quartz Crystal Microbalance (QCM-P) Device

Esmaeilzadeh, Hamed; Su, Jun-Wei; Ji, Siqi; Cernigliaro, George; Gehring, Frank K.; Sun, Hongwei

doi:10.1109/jsen.2019.2916102

Cited by 9 publications

(4 citation statements)

References 46 publications

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“…(A) Schematic of one unit of micropillar QCM-P during BSA adsorption (left), commercial device and QCM-F and QCM-P fabricated on 3T qCell quartz crystals (right) with the side view of the micropillar arrays; (B) frequency responses for QCM-P with different concentrations of BSA adsorption between 0.3 and 100 μg/mL on superhydrophilic micropillar surfaces using the above-mentioned system. Adapted with permission from ref . Copyright 2019 IEEE.…”

Section: An Overview Of Biomacromolecular Characterizations Using Qcm-dmentioning

confidence: 99%

“…A highly sensitive QCM-D instrument is vital for biosensor applications. Sun and co-workers 50 demonstrated that using a crystal surface modified with a polymer micropillar array (QCM-P, 3T qCell) instead of polymer film (QCM-F, traditional QCM from ICM) can lower the detection limit further. Figure 5A shows the schematic of one unit micropillar of QCM-P during BSA adsorption, the commercial QCM device with differently modified crystals, and the side view of the micropillar arrays.…”

Section: Characterizations Using Qcm-dmentioning

confidence: 99%

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Biomacromolecular Characterizations Using State-of-the-Art Quartz Crystal Microbalance with Dissipation

Wei,

Shao,

Qiao

et al. 2023

Anal. Chem.

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Biomolecular characterization is essential in fields such as drug discovery, glycomics, and cell biology. This feature article focuses on the experimental use of quartz crystal microbalance with dissipation (QCM-D) as a powerful analytical technique to probe biological events ranging from biomacromolecular interactions and conformational changes of biomacromolecules to surface immobilization of biomacromolecules and cell morphological changes.

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Section: An Overview Of Biomacromolecular Characterizations Using Qcm-dmentioning

confidence: 99%

Section: Characterizations Using Qcm-dmentioning

confidence: 99%

Biomacromolecular Characterizations Using State-of-the-Art Quartz Crystal Microbalance with Dissipation

Wei,

Shao,

Qiao

et al. 2023

Anal. Chem.

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“…Quartz Crystal Microbalances (QCMs) are sensors based on piezoelectric electromechanical resonators, which interact with the environment and change their resonant behavior due to different interaction mechanisms. QCMs-based sensing systems have a wide range of applications, such as gas sensing, humidity sensing, particle sensing, biosensors for a variety of biological targets, film growth monitoring in electrochemical deposition and liquid viscosity sensing [ 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 ]. The use of QCM has some consolidated application fields but is still a subject for many research works aiming at improving performance from different points of view.…”

Section: Introductionmentioning

confidence: 99%

Strategies for the Accurate Measurement of the Resonance Frequency in QCM-D Systems via Low-Cost Digital Techniques

Addabbo

Fort

Landi

et al. 2022

Sensors

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In this paper, an FPGA (Field Programmable Gate Array)-based digital architecture for the measurement of quartz crystal microbalance (QCM) oscillating frequency of transient responses, i.e., in QCM-D (QCM and Dissipation) applications, is presented. The measurement system is conceived for operations in liquid, with short QCM transient responses due to the large mechanical load. The proposed solution allows for avoiding the complex processing systems typically required by the QCM-D techniques and grants frequency resolutions better than 1 ppm. The core of the architecture is a reciprocal digital frequency meter, combined with the preprocessing of the QCM signal through mixing operations, such as a step-down of the input frequency and reducing the measurement error. The measurement error is further reduced through averaging. Different strategies are proposed to implement the proposed measurement solution, comprising an all-digital circuit and mixed analog/digital ones. The performance of the proposed architectures is theoretically derived, compared, and analyzed by means of experimental data obtained considering 10 MHz QCMs and 200 ms long transient responses. A frequency resolution of about 240 ppb, which corresponds to a Sauerbrey mass resolution of 8 ng/cm2, is obtained for the all-digital solution, whereas for the mixed solution the resolution halves to 120 ppb, with a measurement time of about one second over 100 repetitions.

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“…The electromechanical models exist in the form of the equivalent-circuit model, while the mechanical models take the form of static or dynamic behavior models. Based on these approaches, simplified representations of micropillars have appeared in the form of either discrete model (single/two-degree [s] of freedom), as done in 45,53 or continuum model as done in. 21,25,54 In all, each of the modeling routes has its strengths and weaknesses, and the predictive power of any derived model is limited by the underlying assumptions.…”

mentioning

confidence: 99%

Modeling and analysis of nature‐inspired branched micropillars for enhanced dynamic bio‐sensing

Mustapha

Hawwa

Abakr

2021

Numer Methods Biomed Eng

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Research evidence abounds on the effectiveness of micropillar‐based microelectromechanical systems for the detection of a wide variety of ultrasmall biological objects for clinical and non‐clinical applications. However, the standard micropillar‐based sensing platforms rely on a single‐column micropillar with a spot at the tip for binding of objects. Although this long‐standing form has shown immense potential, performance improvement is hindered by the fundamental limits enforced by physical laws. Moreover, the single‐column micropillar has a lower sensing area and is ill‐suited for a simultaneous differential sensing of chemical/biological objects of different mass. Here, we report a new set of nature‐inspired, branched micropillar‐based sensing resonators to address the highlighted issues. The characteristics of the newly proposed branched micropillars are comprehensively examined with three payloads (Bartonella Bacilliformis, Escherichia coli, and Micro magnetic beads). Anchored on the capability of continuum theoretical framework, the mathematical model of the micropillar is formulated through the synthesis of the modified couple stress, the Rayleigh‐Love, and the Timoshenko theories. The finite element method is employed to shed light on the variability of the structures' resonant response under performance reduction factors (payload's rotary inertia, damaged substrate, and density of a surrounding fluid). The results obtained indicate superior performance indicators for the triply‐branched micropillar: enhanced response sensitivity for multiple payloads and less susceptibility to deterioration in resonant frequencies due to fluid immersion.

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Lowest Detectable Protein Immobilization Measurement Using an Ultrasensitive Micropillar-Based Quartz Crystal Microbalance (QCM-P) Device

Cited by 9 publications

References 46 publications

Biomacromolecular Characterizations Using State-of-the-Art Quartz Crystal Microbalance with Dissipation

Biomacromolecular Characterizations Using State-of-the-Art Quartz Crystal Microbalance with Dissipation

Strategies for the Accurate Measurement of the Resonance Frequency in QCM-D Systems via Low-Cost Digital Techniques

Modeling and analysis of nature‐inspired branched micropillars for enhanced dynamic bio‐sensing

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