Low STYK1 expression indicates poor prognosis in gastric cancer

Abstract

BackgroundThe expression of serine threonine tyrosine kinase 1 (STYK1), a member of the receptor protein tyrosine kinase (RPTK) family, is abnormal in several cancers. However, the molecular mechanism of STYK1 regulation of gastric cancer (GC) progression is unknown.Materials and methodsWe evaluated STYK1 expression in GC tissues and the corresponding normal tissues. Specimens from 93 patients with GC were examined with immunohistochemical staining. The relationship between STYK1 protein expression and the patients’ clinicopathological features was assessed. Kaplan–Meier and Cox proportional regression analyses were used to evaluate the association between STYK1 expression and survival.ResultsSTYK1 expression was decreased in GC tissues. Low STYK1 expression was significantly associated with poor tumor differentiation (P=0.023), advanced clinical stage (P=0.021), and poor overall survival (OS; P=0.034). Univariate and multivariate analyses revealed that STYK1expression was an independent prognostic indicator (HR =0.53, 95% CI =0.29–0.95, P=0.039; HR =0.51, 95% CI =0.24–0.91, P=0.030, respectively).ConclusionDownregulated STYK1 expression correlated significantly with poor tumor differentiation, advanced clinical stage, and poor OS in GC. STYK1 might be a diagnostic and prognostic indicator in patients with GC.

STYK1/NOK Promotes Metastasis and Epithelial-Mesenchymal Transition in Non-small Cell Lung Cancer by Suppressing FoxO1 Signaling

“…Our results confirmed that STYK1 was overexpressed in NSCLC, indicating that STYK1 could play a significant role in NSCLC progression. This tendency is consistent with STYK1 overexpression in many other malignancies ( Moriai et al, 2006 ; Jackson et al, 2009 ; Kondoh et al, 2009 ; Orang et al, 2014 ; Chen et al, 2016 , 2017 ; Hu et al, 2018 ), except for gastric cancer ( Fang et al, 2018 ) and some cases of castration-naïve prostate cancer ( Chung et al, 2009 ). In addition, in vitro and in vivo experiments demonstrated that the upregulation of STYK1 expression caused epithelial NSCLC cells to acquire mesenchymal features, to migrate, and to invade, which suggests that STYK1 facilitates metastasis of NSCLC cells to distant sites.…”

Identification of gene signatures associated with ulcerative colitis and the association with immune infiltrates in colon cancer

“…IHC was performed as describe previously ( 16 ). Briefly, the tissues were exposed to primary antibodies like macrophage inflammatory protein-1α (CCL3; 1:200, DF8572, Affinity), MMP3 Monoclonal antibody (1:200, 66338-1-Ig, Proteintech), and TIMP1 polyclonal antibody (1:200, 16644-1-AP, Proteintech).…”

“…Western blotting was performed as described previously ( 16 ). The membranes/blot were incubated with primary antibodies like CCL3, MMP3, TIMP1, and GAPDH (CCL3; 1:1000, DF8572, Affinity; MMP3 Monoclonal antibody, 1:5000, 66338-1-Ig, Proteintech; TIMP1 polyclonal antibody, 1:1000, 16644-1-AP, Proteintech; GAPDH, 1:20000, 60004-1-Ig, Proteintech; Rainbow 180 plus Marker, 39.VPM003, VICMED; Universal antibody diluent, 39.VP6022-50, VICMED).…”

TIMP2 is associated with prognosis and immune infiltrates of gastric and colon cancer

“…The detailed procedures for the immunohistochemical experiments are described above (20), the tissue section was treated with primary antibodies against TIMP2 (sc-21735, working dilution 1: 50, Santa Cruz Biotechnology, Inc., USA).…”