Low-molecular-weight thiol transferases in redox regulation and antioxidant defence

Tossounian, Maria-Armineh; Zhao, Yuhan; Yu, Bess Yi Kun; Markey, Samuel A.; Malanchuk, Oksana; Zhu, Yuejia; Cain, Amanda; Gout, Ivan

doi:10.1016/j.redox.2024.103094

Cited by 3 publications

(1 citation statement)

References 199 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, a trend to display a higher thiol pool was shown in cells growing in inositol as carbon source was observed compared with glucose. Low-molecular-weight thiols are a heterogeneous group of molecules with a highly reactive sulfhydryl group present in the different groups of living cells [ 61 ]. The most representative members of this group include glutathione, cysteine and coenzyme A [ 62 ].…”

Section: Discussionmentioning

confidence: 99%

Nitrosative stress under microaerobic conditions triggers inositol metabolism in Pseudomonas extremaustralis

Venero,

Giambartolomei,

Sosa

et al. 2024

PLoS ONE

View full text Add to dashboard Cite

Bacteria are exposed to reactive oxygen and nitrogen species that provoke oxidative and nitrosative stress which can lead to macromolecule damage. Coping with stress conditions involves the adjustment of cellular responses, which helps to address metabolic challenges. In this study, we performed a global transcriptomic analysis of the response of Pseudomonas extremaustralis to nitrosative stress, induced by S-nitrosoglutathione (GSNO), a nitric oxide donor, under microaerobic conditions. The analysis revealed the upregulation of genes associated with inositol catabolism; a compound widely distributed in nature whose metabolism in bacteria has aroused interest. The RNAseq data also showed heightened expression of genes involved in essential cellular processes like transcription, translation, amino acid transport and biosynthesis, as well as in stress resistance including iron-dependent superoxide dismutase, alkyl hydroperoxide reductase, thioredoxin, and glutathione S-transferase in response to GSNO. Furthermore, GSNO exposure differentially affected the expression of genes encoding nitrosylation target proteins, encompassing metalloproteins and proteins with free cysteine and /or tyrosine residues. Notably, genes associated with iron metabolism, such as pyoverdine synthesis and iron transporter genes, showed activation in the presence of GSNO, likely as response to enhanced protein turnover. Physiological assays demonstrated that P. extremaustralis can utilize inositol proficiently under both aerobic and microaerobic conditions, achieving growth comparable to glucose-supplemented cultures. Moreover, supplementing the culture medium with inositol enhances the stress tolerance of P. extremaustralis against combined oxidative-nitrosative stress. Concordant with the heightened expression of pyoverdine genes under nitrosative stress, elevated pyoverdine production was observed when myo-inositol was added to the culture medium. These findings highlight the influence of nitrosative stress on proteins susceptible to nitrosylation and iron metabolism. Furthermore, the activation of myo-inositol catabolism emerges as a protective mechanism against nitrosative stress, shedding light on this pathway in bacterial systems, and holding significance in the adaptation to unfavorable conditions.

show abstract

Section: Discussionmentioning

confidence: 99%

Nitrosative stress under microaerobic conditions triggers inositol metabolism in Pseudomonas extremaustralis

Venero,

Giambartolomei,

Sosa

et al. 2024

PLoS ONE

View full text Add to dashboard Cite

show abstract

The role of thioredoxin system in the maintenance of redox homeostasis in Mycobacterium tuberculosis

Sugandhi,

Yadav,

Tiwari

et al. 2024

Proc.Indian Natl. Sci. Acad.

View full text Add to dashboard Cite

Protective Role of Melatonin Against Abamectin-Induced Biochemical, Immunohistochemical, and Ultrastructural Alterations in the Testicular Tissues of Rats

Adiguzel,

Karaboduk,

Uzunhisarcikli

2024

Microscopy and Microanalysis

View full text Add to dashboard Cite

Abamectin is one of the most widely used pesticides due to its strong insecticidal and anthelmintic activities. Melatonin is a neurohormone with potent antioxidant, anti-apoptotic, and anti-inflammatory effects. This study aimed to investigate the potential ameliorative effects of melatonin against abamectin-induced testicular toxicity in rats. Twenty-four rats were divided into four groups: control group (1 mL/kg/day corn oil), melatonin-treated group (10 mg/kg/day), abamectin-treated group (0.5 mg/kg/day), and melatonin plus abamectin-treated group. Test substances were administered via oral gavage once daily for 28 days. While MDA and 8-OHdG levels increased in the testicular tissue of rats treated with abamectin, SOD, CAT, GPx, and GST enzyme activities decreased significantly. While interleukin-17 levels, TNF-α, and caspase3 expression increased in the testicular tissue, acetylcholinesterase activity decreased. At the same time, serum gonadotropins (luteinizing and follicle-stimulating hormones) and testosterone levels decreased. Light microscope examinations of testicular tissues revealed severe histopathological changes, such as atrophic hyalinized seminiferous tubules, basement membrane irregularity, degeneration, spermatogenic cell loss, and necrosis. Electron microscopy examinations revealed large vacuoles in Sertoli and spermatogenic cells, swelling and vacuolization in mitochondria, lysosomal structures, and increased pyknotic nuclei. In contrast, melatonin supplementation significantly ameliorated abamectin-induced testicular toxicity in rats through antioxidant, antiapoptotic, and anti-inflammatory mechanisms.

show abstract

Low-molecular-weight thiol transferases in redox regulation and antioxidant defence

Cited by 3 publications

References 199 publications

Nitrosative stress under microaerobic conditions triggers inositol metabolism in Pseudomonas extremaustralis

Nitrosative stress under microaerobic conditions triggers inositol metabolism in Pseudomonas extremaustralis

The role of thioredoxin system in the maintenance of redox homeostasis in Mycobacterium tuberculosis

Protective Role of Melatonin Against Abamectin-Induced Biochemical, Immunohistochemical, and Ultrastructural Alterations in the Testicular Tissues of Rats

Contact Info

Product

Resources

About