Low light intensity and nitrogen starvation modulate the chlorophyll content of <i>Scenedesmus dimorphus</i>

Ferreira, Veronica da Silva; Pinto, Roberta F.; Sant’Anna, Celso

doi:10.1111/jam.13007

Cited by 78 publications

(41 citation statements)

References 40 publications

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“…It has been shown that high intensity of light exhibits inhibition of pigment content while growing algal cells . It is also reported that low light availability enhanced the chlorophyll content and vice versa . Therefore, it may be stated that these two strains are adopted under low light intensity and accordingly adopted their metabolic strategy.…”

Section: Discussionmentioning

confidence: 99%

Biochemical characterization of microalgae collected from north east region of India advancing towards the algae‐based commercial production

Ghosh

Khanra

Mondal

et al. 2017

Asia-Pacific J Chem Eng

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Selection of suitable strain of microalgae is the crucial factor for large-scale production of algae-based products. Efforts have been made here for isolation, identification and biochemical characterization of five microalgae strains collected from Tripura (a small state in north-eastern region of India). Two Chlorococcum sp. (NITAAP008 and NITAAP019) demonstrate their high lipid (15-24%), equal amounts of carbohydrate and protein (35-40%), with specific growth rate of 0.13 day À1 . These strains are potential resource for biofuel production. After lipid extraction, remaining biomass can be used as source of carbohydrate for the production of other biofuels. One isolated strain is identified as Chlorella sp. (NITAAP009) and shows 22-33% carbohydrate, 41-50% protein and 5% chlorophyll with specific growth rate of 0.125 day À1 . Another Chlorella sp. (NITAAP011) isolated from lake area exhibits significant chlorophyll (5-6.4%), 30-50% carbohydrate, 48-60% protein and low lipid (1-10%) with lower specific growth rate (0.10 day À1 ). Both strains are having industrial competence for chlorophyll production due to their synthesizing ability of significant amount of chlorophyll (5-6.4%). The last one, Korshikoviella sp. (NITAAP017), has 15-18% lipid, 22-34% carbohydrate, 30-43% protein and 3-4% chlorophyll with specific growth rate of 0.12 day À1 and can be used for food supplement production or lipid synthesis.

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Section: Discussionmentioning

confidence: 99%

Biochemical characterization of microalgae collected from north east region of India advancing towards the algae‐based commercial production

Ghosh

Khanra

Mondal

et al. 2017

Asia-Pacific J Chem Eng

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“…In this case, Ferreira et al . () demonstrated an increase in chlorophyll content at 16.91 ± 0.45

μ

mol photons m −2 s −1 to capture light in a more efficient manner in Scenedesmus dimorphus (UTEX 1237); and Pal et al . () indicated that 1000–1500 lux (6–9

μ

E m −2 s −1 ) were the best light intensities in order to produce maximum yield in Chaetoceros muelleri (CS‐178).…”

Section: Resultsmentioning

confidence: 99%

A novel method to analyse in vivo the physiological state and cell viability of phototrophic microorganisms by confocal laser scanning microscopy using a dual laser

Millach

Obiol

Solé

et al. 2017

Journal of Microscopy

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Phototrophic microorganisms are very abundant in extreme environments, where are subjected to frequent and strong changes in environmental parameters. Nevertheless, little is known about the physiological effects of these changing environmental conditions on viability of these microorganisms, which are difficult to grow in solid media and have the tendency to form aggregates. For that reason, it is essential to develop methodologies that provide data in short time consuming, in vivo and with minimal manipulating the samples, in response to distinct stress conditions. In this paper, we present a novel method using Confocal Laser Scanning Microscopy and a Dual Laser (CLSM-DL) for determining the cell viability of phototrophic microorganisms without the need of either staining or additional use of image treating software. In order to differentiate viable and nonviable Scenedesmus sp. DE2009 cells, a sequential scan in two different channels was carried out from each same xyz optical section. On the one hand, photosynthetic pigments fluorescence signal (living cells) was recorded at the red channel (625- to 785-nm fluorescence emission) exciting the samples with a 561-nm laser diode, and an acousto-optic tunable filter (AOTF) of 20%. On the other hand, nonphotosynthetic autofluorescence signal (dead cells) was recorded at the green channel (500- to 585-nm fluorescence emission) using a 405-nm UV laser, an AOTF of 15%. Both types of fluorescence signatures were captured with a hybrid detector. The validation of the CLSM-DL method was performed with SYTOX green fluorochrome and electron microscopic techniques, and it was also applied for studying the response of distinct light intensities, salinity doses and exposure times on a consortium of Scenedesmus sp. DE2009.

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“…The elevated chlorophyll content in NSAID treated cultures at the end of the extended exposure (at the 28 th day) was an unexpected phenomenon. Chlorophyll content of microalgal cells could be increased mainly by low light intensity [76][77][78][79], and can be interpreted as shade-adaptation [80]. Although cysts tend to sink to the bottom, which could lead to reduced light availability, the lack of increase in chlorophyll content of all other cultures than NSAID treated ones excludes likely this explanation.…”

Section: Analysis Of Pigment Contentmentioning

confidence: 99%

Non-steroidal anti-inflammatory drugs initiate morphological changes but inhibit carotenoid accumulation in Haematococcus pluvialis

et al. 2018

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Low light intensity and nitrogen starvation modulate the chlorophyll content of Scenedesmus dimorphus

Abstract: The findings acquired in this work are of great biotechnological importance to extend knowledge of choosing the right culture condition to stimulate the effectiveness of microalgae strains for chlorophyll production purposes.

Cited by 78 publications

References 40 publications

Biochemical characterization of microalgae collected from north east region of India advancing towards the algae‐based commercial production

Biochemical characterization of microalgae collected from north east region of India advancing towards the algae‐based commercial production

A novel method to analyse in vivo the physiological state and cell viability of phototrophic microorganisms by confocal laser scanning microscopy using a dual laser

Non-steroidal anti-inflammatory drugs initiate morphological changes but inhibit carotenoid accumulation in Haematococcus pluvialis

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