Low doses of X-ray irradiation influence the liver detoxication system in rats with transplanted Guerin’s carcinoma

Марченко, М. М.; Kopylchuk, G. P.; Ketsa, O. V.

doi:10.1134/s1990750809040076

Cited by 4 publications

(5 citation statements)

References 16 publications

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“…In contrast, cytochrome P450 (Table 2) and glutathione transferase (Table 3) activities in the liver microsomal fractions of radiation-exposed rats were reduced in the latent and logarithmic phases of oncogenesis compared with non-irradiated rats having tumors. In this case, the radiation did not influence the enzyme activity of liver cytochrome P450 and glutathione transferase in the terminal stages of Guerin’s carcinoma growth [57]. …”

Section: Tabular Presentationmentioning

confidence: 99%

Summary of Information on the Effects of Ionizing and Non-ionizing Radiation on Cytochrome P450 and Other Drug Metabolizing Enzymes and Transporters

Rendić

Guengerich²

2012

CDM

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The present paper is an update of data on the effects of ionizing radiation (γ-rays, X-rays, high energy UV, fast neutron) caused by environmental pollution or clinical treatments and the effects of non-ionizing radiation (low energy UV) on the expression and/or activity of drug metabolism (e.g., cytochrome P450,, glutathione transferase), enzymes involved in oxidative stress (e.g., peroxidases, catalase,, aconitase, superoxide dismutase), and transporters. The data are presented in tabular form (Tables 1–3) and are a continuation of previously published summaries on the effects of drugs and other chemicals on cytochrome P450 enzymes (Rendic, S.; Di Carlo, F. Drug Metab. Rev., 1997, 29 (1–2), 413–580, Rendic, S. Drug Metab. Rev., 2002, 34 (1–2), 83–448) and of the data on the effects of diseases and environmental factors on the expression and/or activity of human cytochrome P450 enzymes and transporters (Guengerich, F.P.; Rendic, S. Curr. Drug Metab., 2010, 11(1), 1–3, Rendic, S.; Guengerich, F.P. Curr. Drug Metab., 2010, 11 (1), 4–84). The collective information is as presented by the cited author(s) in cases where several references are cited the latest published information is included. Remarks and conclusions suggesting clinically important impacts are highlighted, followed by discussion of the major findings. The searchable database is available as an Excel file (for information about file availability contact the corresponding author).

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Section: Tabular Presentationmentioning

confidence: 99%

Summary of Information on the Effects of Ionizing and Non-ionizing Radiation on Cytochrome P450 and Other Drug Metabolizing Enzymes and Transporters

Rendić

Guengerich²

2012

CDM

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“…Предыдущими исследованиями показано, что при росте в организме карциномы Герена происходит нарушение окислительно-восстановительных процессов в микросомной гидроксилазной цепи, что, вероятно, определяет нарушения функций данного органа [4]. Анализ полученных результатов показал, что действие на организм фракционированного рентгеновского облучения в малых дозах приводит к повышению ферментативной активности NADH-цитохром b 5 редуктазы и одновременному снижению содержания цитохрома b 5 на первые и седьмые сутки после снятия радиационного фактора.…”

Section: рисунокunclassified

“…По мере отдаления от срока облучения -на 21-е сутки, действие малых доз радиации нивелируется и исследуемые показатели приближаются к значениям интактных животных (рис. 4,5).…”

Section: рисунокunclassified

“…Развитие в организме патологических процессов, в том числе онкологических, или влияние на организм разных факторов окружающей среды могут быть причиной изменений ферментативной активности NADH-цитохром b 5 редуктазы, цитохрома b 5 и, как следствие, цитохрома Р450 [4]. Изучение каталитических свойств NADH-специфического флавопротеина и цитохрома b 5 в условиях роста в организме злокачественного новообразования имеет большое значение для понимания нарушения транспорта электронов на CYP и процессов гидроксилирования ксенобиотиков разными изоформами CYP в печени.…”

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Functional activity of NADH-dependent reductase system in liver and guerin’s carcinoma microsomal fraction in rats exposed to preliminary irradiation

2013

BIOMED KHIM

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В работе проведено исследование ферментативной активности NADH-цитохром b 5 редуктазы, содержания и скорости восстановления-окисления цитохрома b 5 в микросомной фракции печени и карциномы Герена предварительно облученных крыс-опухоленосителей. Показано, что облучение организма, предшествующее трансплантации опухоли, приводит к снижению как ферментативной активности NADH-цитохром b 5 редуктазы, так и содержания цитохрома b 5 в микросомной фракции карциномы Герена в латентную и логарифмическую фазы онкогенеза в сравнении с необлученными опухоленосителями.Установлено, что ферментативная активность NADH-цитохром b 5 редуктазы в микросомной фракции печени облученных крыс в условиях роста в организме карциномы Герена повышается в латентную и логарифмическую фазы онкогенеза. В то же время, снижается содержание цитохрома b 5 с одновременным повышением скорости его восстановления-окисления в микросомной фракции печени облучённых крыс-опухоленосителей. (O 2 2-), что имеет большое значение в метаболизме ксенобиотиков, в том числе лекарственных препаратов [2,3].Нарушение структурно функциональных особенностей NADH-зависимой цепи ЭР может привести к изменениям функционирования монооксигеназной системы в целом. Развитие в организме патологических процессов, в том числе онкологических, или влияние на организм разных факторов окружающей 662 * -адресат для переписки

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“…Investigations into changes in the activity and expression of cytochrome P450 due to X-ray irradiation are scarce. The effects of X-ray irradiation exposure on CYP3A1 and carboxylesterase (CES) expression in rats have recently been investigated by our team (Marchenko et al, 2010;Qiao et al, 2019). However, no reports on changes in CYP1A2 and CYP2E1 expression levels and activity due to X-ray irradiation have been published to date.…”

Section: Introductionmentioning

confidence: 99%

Regulation of X-Ray Irradiation on the Activity and Expression Levels of CYP1A2 and CYP2E1 in Rats

Wang

et al. 2020

Front. Pharmacol.

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The objective of this study was to investigate the regulation of X-ray irradiation and its effect on the activity and protein and mRNA expression levels of CYP1A2 and CYP2E1 in rats. Rats were randomly divided into 0 Gy (control), 1 Gy (low-dose irradiation), and 5 Gy (high-dose irradiation) groups. CYP1A2 and CYP2E1 activity was evaluated from changes in pharmacokinetic parameters of caffeine and chlorzoxazone, respectively. The plasma concentrations of the probe drugs were determined by high-performance liquid chromatography (HPLC). Enzyme-linked immunosorbent assay (ELISA) and real-time polymerase chain reaction (PCR) tests were used to analyze the protein and mRNA expression levels of CYP1A2 and CYP2E1, respectively. The AUC 0-12 of caffeine was decreased by 1.7-and 2.5-fold, and the CL was increased by 1.8-and 2.6-fold in the 1 Gy and 5 Gy groups, respectively, compared to the 0 Gy group. The AUC 0-10 of chlorzoxazone was 1.4-and 1.8-fold lower, and the CL was 1.4-and 1.9-fold higher in the 1 Gy and 5 Gy groups, respectively, compared to the 0 Gy group. The metabolism of caffeine and chlorzoxazone increased under X-ray irradiation as CL levels increased and AUC levels decreased, suggesting that CYP1A2 and CYP2E1 activity is enhanced in rats after X-ray irradiation. Compared to that of the 0 Gy group, the protein expression level of CYP1A2 was measured as 28.3% and 38.9% higher in the 1 Gy and 5 Gy groups, respectively. The protein expression level of CYP2E1 was 48.4% higher in the 5 Gy group compared to the 0 Gy group, and there was no statistically significant difference between 0 Gy and 1 Gy. Compared to the 0 Gy group, the mRNA expression level of CYP1A2 was 200% and 856.3% higher in the 1 Gy and 5 Gy group, respectively, whereas the mRNA expression level of CYP2E1 was 89.0% and 192.3% higher in the 1 Gy and 5 Gy groups, respectively. This study reveals significant changes in the activity and protein and mRNA expression levels of CYP1A2 and CYP2E1 in rats after exposure to X-ray irradiation. AnimalsSprague Dawley SPF rats (200 ± 20 g, certificate No. 2007-001) of both sexes were provided by the laboratory animal center at Xi'an Jiaotong University Medical College, China. They were adapted for a week at 23 ± 2°C with a constant humidity level of 55% ± 5% under a cycle of 12 h of dark conditions and given ad libitum Li et al. X-Ray Irradiation and CYP1A2 and CYP2E1 Frontiers in Pharmacology | www.frontiersin.org January 2020 | Volume 10 | Article 1575 2

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Low doses of X-ray irradiation influence the liver detoxication system in rats with transplanted Guerin’s carcinoma

Cited by 4 publications

References 16 publications

Summary of Information on the Effects of Ionizing and Non-ionizing Radiation on Cytochrome P450 and Other Drug Metabolizing Enzymes and Transporters

Summary of Information on the Effects of Ionizing and Non-ionizing Radiation on Cytochrome P450 and Other Drug Metabolizing Enzymes and Transporters

Functional activity of NADH-dependent reductase system in liver and guerin’s carcinoma microsomal fraction in rats exposed to preliminary irradiation

Regulation of X-Ray Irradiation on the Activity and Expression Levels of CYP1A2 and CYP2E1 in Rats

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