Low dose alpha interferon therapy can be effective in chronic active hepatitis C. Results of a multicentre, randomised trial.

Sánchez-Tapias, José-Marı́a; Forns, Xavier; Ampurdanès, Sergi; Titó, L; Planas, Ramón; Viver, Josep Maria; Acero, Doroteo; Torres, Maribel Rodríguez; Más, P.; Morillas, Rosa; Forné, Montserrat; Espinós, Jaime; Llovet, Josep M.; Costa, J.; Olmedo, E; López‐Labrador, F. Xavier; Anta, M. T. Jiménez de; Rodés, Joan

doi:10.1136/gut.38.4.603

Cited by 11 publications

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“…HCV RNA in serum was determined by reverse transcription (RT) and amplification by nested PCR of the 5′ non-coding region (5′NCR) region of HCV, as described previously 16. HCV RNA serum levels were measured by a competitive PCR method recently developed in our laboratory 17…”

Section: Methodsmentioning

confidence: 99%

Hepatitis G virus infection in chronic liver disease

Guilera

Sáiz

López‐Labrador

et al. 1998

Gut

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Background-The hepatitis G virus (HGV), a recently identified member of the Flaviviridae family, can cause chronic infection in man but the role of this agent in chronic liver disease is poorly understood. Aims-To evaluate the prevalence and meaning of HGV infection in a large series of patients with chronic liver disease. Subjects-Two hundred volunteer blood donors, 179 patients with chronic hepatitis C, 111 with chronic hepatitis B, 104 with alcoholic liver disease, 136 with hepatocellular carcinoma, and 24 with cryptogenic chronic liver disease were studied. Methods-HGV RNA was investigated in serum samples by reverse transcription and polymerase chain reaction amplification of the 5' non-coding region of HCV and hybridisation to a specific probe. The main features of HGV RNA seropositive and seronegative patients were compared. Results-The prevalence of HGV infection was 3% in blood donors, 7% in chronic hepatitis C, 8% in chronic hepatitis B, 2% in alcoholic liver disease, 4% in hepatocellular carcinoma, and 8% in cryptogenic chronic liver disease. HGV infected patients tended to be younger than noninfected patients but no diVerences concerning sex, possible source of infection, clinical manifestations, biochemical and virological parameters, or severity of liver lesions were found. Conclusions-The prevalence of HGV infection in chronic liver disease seems to be relatively low in our area. Infection with HGV does not seem to play a significant pathogenic role in patients with chronic liver disease related to chronic HBV or HCV infection or to increased alcohol consumption, or in those with cryptogenic chronic liver disease. (Gut 1998;42:107-111)

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Section: Methodsmentioning

confidence: 99%

Hepatitis G virus infection in chronic liver disease

Guilera

Sáiz

López‐Labrador

et al. 1998

Gut

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“…HCV-RNA from liver and serum was amplified by RT-PCR using primers derived from the 5Ј-NCR as previously described (Sá nchez-Tapias et al, 1996;Soguero at al, 1999). The HVR-1 was amplified by nested PCR using the following primers: HV7 (outer sense, nt 1396 to 1417) 5Ј-ACT GGG GAG TCC TGG CGG GCC T-3Ј; HV5C (outer antisense, nt 1611 to 1633) 5Ј-TCA TTG CAA TTC AGG GCA GTC CT-3Ј; HV3B (inner sense, nt 1431 to 1453) 5Ј-ATG GTG GGT ACC TGG GCT AAG GT-3Ј; and HV6B (inner antisense, nt 1598 to 1621) 5Ј-AGG GAA TTC CTG TTG ATG TGC CA-3Ј.…”

Section: Amplification Of Hcv Genomic Regionsmentioning

confidence: 99%

Assessment of Genotype and Molecular Evolution of Hepatitis C Virus in Formalin-Fixed Paraffin-Embedded Liver Tissue from Patients With Chronic Hepatitis C Virus Infection

Soguero

Campo

Ribalta

et al. 2000

Laboratory Investigation

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SUMMARY:Drawbacks of hepatitis C virus (HCV) RNA detection in paraffin-embedded liver tissue have satisfactorily been solved by RT-PCR amplification of the 5Јnon-coding region (5ЈNCR). However, detection of this highly conserved region does not provide information on epidemiological or pathogenetic aspects of HCV infection. This study explores whether other functionally important genetic regions of HCV, such as the hypervariable region 1 (HVR-1) and the interferon sensitivity-determining region (ISDR), can be retrieved from paraffin-embedded liver specimens by RT-PCR, and whether the amplified material is suitable for further molecular analyses. RT-PCR amplification of 5ЈNCR, HVR-1, and ISDR was assessed in RNA extracted from 50 formalin-fixed, paraffin-embedded liver specimens, including 23 needle liver biopsies (11 from patients with non-A, non-B chronic hepatitis diagnosed between 1971 and 1985, 8 from subjects with normal liver histology and 4 from sequential biopsies from a patient with HCV recurrence after liver transplantation), and 27 liver explants from patients undergoing transplantation between 1988 and 1996 (16 with HCV-related cirrhosis and 11 with other disorders). The 5ЈNCR was successfully amplified in 8 of 11 (73%) non-A, non-B chronic hepatitis biopsies and in all of the specimens from patients with serological documentation of HCV infection. There were no false-positive results. HCV genotype was identified by RFLP analysis of the 5ЈNCR in the 13 cases analyzed. HVR-1 and ISDR were amplified in 24 of 28 (86%) samples, which were positive for the 5ЈNCR. Efficient amplification was inversely related to the time of storage. The evolutionary changes of HVR-1 and ISDR were successfully analyzed by direct sequencing of amplificates from the explanted liver and from the sequential liver biopsies in a patient with HCV infection recurrence after transplantation. These observations indicate that paraffin-embedded liver tissue, even when stored for more than 20 years, is appropriate for advanced studies on the molecular biology of HCV. (Lab Invest 2000, 80:851-856).

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Diseases of the Liver and Alimentary Tract

Gent¹

2000

Medical Selection of Life Risks

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Low dose alpha interferon therapy can be effective in chronic active hepatitis C. Results of a multicentre, randomised trial.

Cited by 11 publications

References 45 publications

Hepatitis G virus infection in chronic liver disease

Hepatitis G virus infection in chronic liver disease

Assessment of Genotype and Molecular Evolution of Hepatitis C Virus in Formalin-Fixed Paraffin-Embedded Liver Tissue from Patients With Chronic Hepatitis C Virus Infection

Diseases of the Liver and Alimentary Tract

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