Low-cost shoot multiplication and improved growth in different cultivars of Canna indica

Abstract

Canna (Canna indica L.) is an ornamental landscape plant used specially for the garden borders and beds. It grows in tropical and subtropical countries including India. Canna is a less explored crop, mainly because it is a slow growing monocot with extremely hard seed coat and difficult to establish in vitro, as bacterial contamination is carried through the soil-grown rhizome. Many cultivars (ca. 150) of canna are being maintained in the garden germplasm of National Botanical Research Institute. To obtain 100% in vitro seed germination, chipping off of seeds with a sterilized nail clipper and soaking for 24-48 h or until radical emergence was a prerequisite. To obtain a foolproof tissue culture protocol of canna, in the present study, shoot multiplication was obtained through rhizome axillary buds. Among semisolid, liquid submerged and liquid media with glass beads, the highest multiplication of shoots (10) was obtained in liquid media with glass beads in 'Canna Flaccida' cv. within 6 weeks of culture incubation. During a comparative analysis of shoot regeneration among ten most attractive selected cultivars of canna, two did not respond, whereas a significant difference was obtained among eight cultivars. The regenerated shoots were rooted, acclimatized, and transferred to the pots, where they grew normally.

Biomimetic Superhydrophobic Materials Construct from Binary Structure: A Review on Design, Properties, and Applications

“…India canna is one of the most popular flowering ornamental plants for garden borders and grows in tropical and subtropical countries, including India. [ 69 ] It is another plant showing a good superhydrophobic property of 165° ± 2°. In addition to these two plants, there are many other creatures with hierarchical micro‐ and nanostructures, such as the famous self‐cleaning plants like lotus leaf, rice leaf, and so on.…”

Potential of Canna indica in Constructed Wetlands for Wastewater Treatment: A Review

“…When compared to Cyperus alternifolius and Phragmites australis, Canna indica outperforms them in terms of pollution reduction and greenhouse gas emissions [18,27]. Canna indica have a fibrous roots structure that produces high aerobic conditions throughout the CW, allowing for more removal [26].…”

“…Canna indica L. (Figure 1) is one of the most common flowering ornamental plants for garden borders and beds, and occasionally grown as potted plants [27]. Recent investigations have revealed that the plant has medicinal value in addition to its ornamental value [29].…”

Somatic embryo induction and plantlet regeneration of Canna × generalis from immature zygotic embryo

“…However, the regeneration system produced by the traditional organotype method also had some defects. For example, the plants were seriously polluted in the process of culture, the browning rate was high, and the reproduction coe cient and the differentiation rate were low (Purshottam et al 2019;Mitrofanova et al 2017). Previous studies have reported that the leaves of C. indica L. were used to induce callus and regenerated plants by somatic embryogenesis.…”