Low-Cost and Scalable Platform with Multiplexed Microwell Array Biochip for Rapid Diagnosis of COVID-19

Wang, Yang; Li, Kaiju; Xu, Gaolian; Chen, Chuan; Song, Guiqin; Dong, Zaizai; Lin, Long; Wang, Yu; Xu, Zhiyong; Mu, Yu; Yu, Xinge; Ying, Binwu; Fan, Yubo; Chang, Lingqian; Geng, Jia

doi:10.34133/2021/2813643

Cited by 15 publications

(10 citation statements)

References 45 publications

(26 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…146 In addition to singlecell gene sequencing, the combination of microdroplet and isothermal amplification technology provides a simple method for the detection of specific gene sequences in single cells. [147][148] Guo and coworkers employed the DNA hybridization chain reaction to probe the target miRNA in single cells. 149 The miRNAs released from single cells interacted with the encapsulated DNA amplifiers in the microdroplets.…”

Section: Microdroplets For Single-cell Isolation and Analysismentioning

confidence: 99%

Advanced techniques for gene heterogeneity research: Single‐cell sequencing and on‐chip gene analysis systems

Dong

Wang

Yin

et al. 2022

VIEW

Self Cite

View full text Add to dashboard Cite

Gene heterogeneity leads to the differences in cellular behaviors in a wide range, such as tumor drug‐resistant mutation, epithelial‐mesenchymal transition, and migration, posing significant challenges to the development of biomedicine. Traditional gene analysis methods, such as polymerase chain reaction, employ a mass of cells as the gene source, resulting in that the gene properties from a specific single cell are hidden in massive gene information. Recent decades have seen the emerging single‐cell gene analysis techniques with their unprecedented opportunities to study gene heterogeneity with high precision and high throughput. In this review, we summarized the state‐of‐the‐art techniques for single‐cell sequencing and on‐chip gene analysis systems. The principles of each technique are introduced in detail, with the focus on the application scenarios in gene heterogeneity research. Looking forward, we also introduced the challenges in current technologies and point out the future direction for facilitating the technical improvement and clinical applications of single‐cell gene analysis techniques.

show abstract

Section: Microdroplets For Single-cell Isolation and Analysismentioning

confidence: 99%

Advanced techniques for gene heterogeneity research: Single‐cell sequencing and on‐chip gene analysis systems

Dong

Wang

Yin

et al. 2022

VIEW

Self Cite

View full text Add to dashboard Cite

show abstract

“…The platform integrated functions of sample preparation, isothermal amplification. The results could be read with naked eyes directly [ 145 ]. The system has been successfully used to detect 130 real clinical samples.…”

Section: Micro/nano Devices For Point-of-care Testing Of Sars-cov-2mentioning

confidence: 99%

“…Copyright 2020, Royal Society of Chemistry. (d) A low-cost isothermal amplification platform with multiplexed microwell array biochip for rapid and visualized detection of SARS-CoV-2 [ 145 ]; Reproduced with permission. Copyright 2020, AAAS.…”

Section: Micro/nano Devices For Point-of-care Testing Of Sars-cov-2mentioning

confidence: 99%

Micro/nano biomedical devices for point-of-care diagnosis of infectious respiratory diseases

Wang

Dong

et al. 2022

Medicine in Novel Technology and Devices

Self Cite

View full text Add to dashboard Cite

Corona Virus Disease 2019 (COVID-19) has developed into a global pandemic in the last two years, causing significant impacts on our daily life in many countries. Rapid and accurate detection of COVID-19 is of great importance to both treatments and pandemic management. Till now, a variety of point-of-care testing (POCT) approaches devices, including nucleic acid-based test and immunological detection, have been developed and some of them has been rapidly ruled out for clinical diagnosis of COVID-19 due to the requirement of mass testing. In this review, we provide a summary and commentary on the methods and biomedical devices innovated or renovated for the quick and early diagnosis of COVID-19. In particular, some of micro and nano devices with miniaturized structures, showing outstanding analytical performances such as ultra-sensitivity, rapidness, accuracy and low cost, are discussed in this paper. We also provide our insights on the further implementation of biomedical devices using advanced micro and nano technologies to meet the demand of point-of-care diagnosis and home testing to facilitate pandemic management. In general, our paper provides a comprehensive overview of the latest advances on the POCT device for diagnosis of COVID-19, which may provide insightful knowledge for researcher to further develop novel diagnostic technologies for rapid and on-site detection of pathogens including SARS-CoV-2.

show abstract

“…Coronavirus disease 2019 (COVID- 19) is a severe pneumonia pandemic caused by a beta coronavirus, severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) [1]. It emerged in late 2019, and then, outbreak all over the world comes about quickly [2]. Researchers have found that SARS-CoV-2 can use its structural glycosylated Spike (S) protein for entry into host cells through binding to angiotensin-converting enzyme 2 (ACE2) receptors [3].…”

Section: Introductionmentioning

confidence: 99%

Biomimetic Alveolus-on-a-Chip for SARS-CoV-2 Infection Recapitulation

et al. 2022

View full text Add to dashboard Cite

SARS-CoV-2 has caused a severe pneumonia pandemic worldwide with high morbidity and mortality. How to develop a preclinical model for recapitulating SARS-CoV-2 pathogenesis is still urgent and essential for the control of the pandemic. Here, we have established a 3D biomimetic alveolus-on-a-chip with mechanical strain and extracellular matrix taken into consideration. We have validated that the alveolus-on-a-chip is capable of recapitulating key physiological characteristics of human alveolar units, which lays a fundamental basis for viral infection studies at the organ level. Using virus-analogous chemicals and pseudovirus, we have explored virus pathogenesis and blocking ability of antibodies during viral infection. This work provides a favorable platform for SARS-CoV-2-related researches and has a great potential for physiology and pathophysiology studies of the human lung at the organ level in vitro.

show abstract

Low-Cost and Scalable Platform with Multiplexed Microwell Array Biochip for Rapid Diagnosis of COVID-19

Cited by 15 publications

References 45 publications

Advanced techniques for gene heterogeneity research: Single‐cell sequencing and on‐chip gene analysis systems

Advanced techniques for gene heterogeneity research: Single‐cell sequencing and on‐chip gene analysis systems

Micro/nano biomedical devices for point-of-care diagnosis of infectious respiratory diseases

Biomimetic Alveolus-on-a-Chip for SARS-CoV-2 Infection Recapitulation

Contact Info

Product

Resources

About