Low coherence digital holography microscopy based on the Lorenz-Mie scattering model

Vandewiele, Stijn; Strubbe, Filip; Schreuer, Caspar; Neyts, Kristiaan; Beunis, Filip

doi:10.1364/oe.25.025853

Cited by 10 publications

(2 citation statements)

References 38 publications

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“…In our application in fluid mechanics, the parametric part u of the object is a set of droplets of ether. Assuming they are homogeneous spheres with a known complex refractive index, their diffraction can be modeled via the Lorenz-Mie theory [19,[37][38][39][40]. This provides an analytic solution for the diffracted electromagnetic fields E di f and H di f scattered by a sphere, in spherical coordinates (r, θ, ϕ) centered on the sphere center.…”

Section: Parametric Step: Model Fitting Of the Lorenz-mie Theorymentioning

confidence: 99%

Reconstruction of in-line holograms: combining model-based and regularized inversion

Berdeu¹,

Flasseur²,

Méès³

et al. 2019

Opt. Express

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In-line digital holography is a simple yet powerful tool to image absorbing and/or phase objects. Nevertheless, the loss of the phase of the complex wavefront on the sensor can be critical in the reconstruction process. The simplicity of the setup must thus be counterbalanced by dedicated reconstruction algorithms, such as inverse approaches, in order to retrieve the object from its hologram. In the case of simple objects for which the diffraction pattern produced in the hologram plane can be modeled using few parameters, a model fitting algorithm is very effective. However, such an approach fails to reconstruct objects with more complex shapes, and an image reconstruction technique is then needed. The improved flexibility of these methods comes at the cost of a possible loss of reconstruction accuracy. In this work, we combine the two approaches (model fitting and regularized reconstruction) to benefit from their respective advantages. The sample to be reconstructed is modeled as the sum of simple parameterized objects and a complex-valued pixelated transmittance plane. These two components jointly scatter the incident illumination, and the resulting interferences contribute to the intensity on the sensor. The proposed hologram reconstruction algorithm is based on alternating a model fitting step and a regularized inversion step. We apply this algorithm in the context of fluid mechanics, where holograms of evaporating droplets are analyzed. In these holograms, the high contrast fringes produced by each droplet tend to mask the diffraction pattern produced by the surrounding vapor wake. With our method, the droplet and the vapor wake can be jointly reconstructed.

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Section: Parametric Step: Model Fitting Of the Lorenz-mie Theorymentioning

confidence: 99%

Reconstruction of in-line holograms: combining model-based and regularized inversion

Berdeu¹,

Flasseur²,

Méès³

et al. 2019

Opt. Express

View full text Add to dashboard Cite

show abstract

“…Electron holography today is a mature research field and widely in use, allowing for molecular imaging [9,10]. Digital holography within the visible wavelength regime has attracted considerable attention where a broad spectrum of light sources has been employed, ranging from gas [11,12], solid state [13] and semiconductor [1,14,15,16] lasers over light emitting diodes (LEDs) [17,18,19,20] to halogen lamps [21]. Recently, ultra-broadband digital holography with sunlight illumination has been demonstrated, employing a new reconstruction algorithm [22].…”

Section: Introductionmentioning

confidence: 99%

3D-printable portable open-source platform for low-cost lens-less holographic cellular imaging

Amann

Witzleben

Breuer

2019

Sci Rep

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Digital holographic microscopy is an emerging, potentially low-cost alternative to conventional light microscopy for micro-object imaging on earth, underwater and in space. Immediate access to micron-scale objects however requires a well-balanced system design and sophisticated reconstruction algorithms, that are commercially available, however not accessible cost-efficiently. Here, we present an open-source implementation of a lens-less digital inline holographic microscope platform, based on off-the-shelf optical, electronic and mechanical components, costing less than $190. It employs a Blu-Ray semiconductor-laser-pickup or a light-emitting-diode, a pinhole, a 3D-printed housing consisting of 3 parts and a single-board portable computer and camera with an open-source implementation of the Fresnel-Kirchhoff routine. We demonstrate 1.55 μm spatial resolution by laser-pickup and 3.91 μm by the light-emitting-diode source. The housing and mechanical components are 3D printed. Both printer and reconstruction software source codes are open. The light-weight microscope allows to image label-free micro-spheres of 6.5 μm diameter, human red-blood-cells of about 8 μm diameter as well as fast-growing plant Nicotiana-tabacum-BY-2 suspension cells with 50 μm sizes. The imaging capability is validated by imaging-contrast quantification involving a standardized test target. The presented 3D-printable portable open-source platform represents a fully-open design, low-cost modular and versatile imaging-solution for use in high- and low-resource areas of the world.

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Joint reconstruction of an in-focus image and of the background signal in in-line holographic microscopy

Berdeu

Thomas

Momey

et al. 2021

Optics and Lasers in Engineering

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Low coherence digital holography microscopy based on the Lorenz-Mie scattering model

Cited by 10 publications

References 38 publications

Reconstruction of in-line holograms: combining model-based and regularized inversion

Reconstruction of in-line holograms: combining model-based and regularized inversion

3D-printable portable open-source platform for low-cost lens-less holographic cellular imaging

Joint reconstruction of an in-focus image and of the background signal in in-line holographic microscopy

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