2015
DOI: 10.1016/j.celrep.2015.10.004
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Abstract: SUMMARY Understanding how chromatin modification regulates development and disease can be limited by available material. Despite recent progress, balancing high-quality and reliable mapping using chromatin-immunoprecipitation-based deep sequencing (ChIP-seq) remains a challenge. We report two techniques, recovery via protection (RP)-ChIP-seq and favored amplification RP-ChIP-seq (FARP-ChIP-seq), that provide reproducible mapping in as few as 500 cells. RP-ChIP-seq allows detection of age-associated epigenetic … Show more

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Cited by 40 publications
(38 citation statements)
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“…[8][9][10][11][12] Moreover, aberrant epigenetic marks have been identified in several hematological malignancies, consistent with clinical evidence that mutations targeting epigenetic regulators promote leukemogenesis. 44 Our study provides the first chromatin accessibility landscape maps for LT-HSCs, ST-HSCs, and MPPs.…”
Section: Chromatin Accessibility Of Enhancer Elements Changes During mentioning
confidence: 57%
See 1 more Smart Citation
“…[8][9][10][11][12] Moreover, aberrant epigenetic marks have been identified in several hematological malignancies, consistent with clinical evidence that mutations targeting epigenetic regulators promote leukemogenesis. 44 Our study provides the first chromatin accessibility landscape maps for LT-HSCs, ST-HSCs, and MPPs.…”
Section: Chromatin Accessibility Of Enhancer Elements Changes During mentioning
confidence: 57%
“…6,7 Several studies suggest that epigenetic mechanisms play an important role in controlling HSC renewal and lineage commitment. [8][9][10][11][12] Understanding the regulatory mechanisms of HSC self-renewal and differentiation is important for both basic stem cell biology and improving the quality of stem cell transplantation in clinical settings.…”
Section: Introductionmentioning
confidence: 99%
“…34 A recent report elegantly demonstrated aging-associated changes in H3K4me3 in mouse lens epithelial cells. 35 A comprehensive view of transcriptional changes induced by loss of Dnmt3a and Dnmt3b and whether de novo DNA methylation plays a role in maintaining lens homeostasis at advanced age (beyond 10 months of age) requires further analysis.…”
Section: Discussionmentioning
confidence: 99%
“…The recent molecular characterization of mouse lens epithelial cell lines (Terrell et al, 2015) and the establishment of protocols for iPSc-based derivation of lens cells (Anchan et al, 2014; Yang et al, 2010) may facilitate their use in ChIP assays, addressing this problem. Modified assays such as “recovery via protection” (RP)-ChIP-seq and favored amplification RP-ChIP-seq, have recently been developed to capture low-abundance chromatin from a single lens, or from a few hundred cells (~500) (Zheng et al, 2015). Because these assays work on single lens tissues, their potential application to embryonic lens tissue now offers new opportunities to investigate epigenetic regulation in early lens development.…”
Section: Protein-nucleic Acid Interaction-profiling For Lens Grnsmentioning
confidence: 99%