Loss of Pollen-S Function in Two Self-Compatible Selections of Prunus avium Is Associated with Deletion/Mutation of an S Haplotype–Specific F-Box Gene

Sonneveld, T.; Tobutt, K. R.; Vaughan, Simon P.; Robbins, Timothy P.

doi:10.1105/tpc.104.026963

Cited by 238 publications

(200 citation statements)

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“…Earlier studies have reported that two SFB alleles, SFB 1 and SFB 2 , are present in peach (Tao et al 2007). Here, two additional alleles, SFB 3 and SFB 4 , have been identified in peach.…”

Section: Discussionmentioning

confidence: 99%

“…It has been reported that loss of self-incompatibility in Prunoideae is mainly attributed to pollen-part mutation. In self-compatible cultivars of sweet cherry and apricot, for example, DNA insertions/deletions as well as point mutations have been detected in SFB genes (Sonneveld et al 2005;Vilanova et al 2006;Marchese et al 2007). However, the finding that stylar part mutation results in self-compatible phenotype has also reported in almond (Ferna´ndez i Martı´et al 2009).…”

Section: Discussionmentioning

confidence: 99%

“…In peach, two different S-haplotypes have been initially identified in cultivars (Hegedu¨s et al 2006;Tao et al 2007). More recently, two additional S-haplotypes have been reported (Hanada et al 2014).…”

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confidence: 99%

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Identification and characterization of S-RNase genes and S-genotypes in Prunus and Malus species

Wang

Korban

et al. 2015

Can. J. Plant Sci.

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. 2015. Identification and characterization of S-RNase genes and S-genotypes in Prunus and Malus species. Can. J. Plant Sci. 95: 213Á225. Most Rosaceae fruit trees such as Prunus and Malus species exhibit gametophytic self-incompatibility that is genetically controlled by the S-locus. In turn, the S-locus contains at least two tightly-linked S-determinant genes, a pistil S-RNase and a pollen SFB. In this study, S-genotypes of 120 cultivated and wild Prunus accessions (peach) and seven wild Malus accessions (crabapple) have been characterized. Among cultivated Prunus genotypes, four S-RNase alleles, designated S 1 , S 2 , S 3 , and S 4 , have been identified, and they share typical structural features of S-RNases from all other self-incompatible Prunus species. Four S-genotypes, S 1 S 2 , S 1 S 3 , S 1 S 4 , and S 2 S 2 , were identified in peach cultivars, while only one S-genotype S 1 S 2 for wild Prunus species. The S 1 S 2 genotype is predominant in peach cultivars, accounting for 58.3% of all evaluated accessions. Similarly, four SFB alleles were identified in peach cultivars and wild accessions. However, all the four SFB alleles encode truncated proteins due to a frame-shift mutation, resulting in loss of hyper-variable and/or variable regions. For Malus species, a total of 14 S-RNase alleles are identified, and of those, two alleles encode truncated proteins. Overall, the genetic variation of both S-RNase and SFB genes in peach is significantly lower than that of S-RNase and SFB genes in self-incompatible Malus and/or Prunus species. The relationship between the genetic variation of SFB genes and the diversification of S-RNase genes in Rosaceae is also discussed.Key words: Prunus, Malus, Self-incompatibility, S-genotypes, S-RNase, Pollen, S-determinant Gu, C., Wang, L., Korban, S. S. et Han, Y. 2015. Identification et caracte´risation des ge`nes de la S-RNase et des ge´notypes-S chez les espe`ces des genres Prunus et Malus. Can. J. Plant Sci. 95: 213Á225. La plupart des arbres fruitiers de la famille des Rosace´es comme ceux des espe`ces des genres Prunus et Malus illustrent une auto-incompatibilite´game´tophytique que controˆle ge´ne´tiquement le locus-S. Ce dernier regroupe au moins deux ge`nes S-de´terminants e´troitement lie´s, un ge`ne S-RNase du pistil et un ge`ne SFB du pollen. Les auteurs ont caracte´rise´le ge´notype-S de 120 obtentions domestique´es et sauvages de peˆcher (Prunus) et de sept obtentions sauvages de pommetier (Malus). Chez les ge´notypes domestique´s de Prunus, ils ont identifie´quatre alle`les du ge`ne S-RNase, baptise´s S 1 , S 2 , S 3 , et S 4 . Ces alle`les partagent les particularite´s structurales communes aux S-RNases des autres espe`ces auto-incompatibles du genre Prunus. Quatre ge´notypes-S, S 1 S 2 , S 1 S 3 , S 1 S 4 , et S 2 S 2 ont aussi e´te´identifie´s chez les cultivars de peˆcher, mais un seul chez les espe`ces sauvages, S 1 S 2 . Le ge´notype S 1 S 2 pre´domine chez les cultivars de peˆcher et repre´sente 58,3 % de toutes les obtentions examine´es. De meˆme, on a ...

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“…Earlier studies have reported that two SFB alleles, SFB 1 and SFB 2 , are present in peach (Tao et al 2007). Here, two additional alleles, SFB 3 and SFB 4 , have been identified in peach.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Identification and characterization of S-RNase genes and S-genotypes in Prunus and Malus species

Wang

Korban

et al. 2015

Can. J. Plant Sci.

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“…Naturally occurring and induced S-locus mutants have proved to be a valuable resource in studies of homomorphic SI systems (Goring et al, 1993;Nasrallah et al, 1994;Sassa et al, 1997;Golz et al, 2000Golz et al, , 2001Ushijima et al, 2004;Sonneveld et al, 2005;Hauck et al, 2006). In heteromorphic SI systems, the genes determining distyly and tristyly have not yet been discovered (Barrett and Shore, 2008;Labonne et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Characterization of X-ray-generated floral mutants carrying deletions at the S-locus of distylous Turnera subulata

2010

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To investigate the genetic architecture of distyly in Turnera subulata and test the hypothesis that a supergene determines distyly, we used X-ray mutagenesis to generate floral mutants. Based upon the crossing design, all progeny were expected to be short-styled. Of 3982 progeny screened, 10 long-styled mutants, one long homostyle and one short homostyle were recovered. Assays for molecular markers tightly linked to the S-locus showed that the mutants were missing 1-3 markers indicating they are deletion mutants. We investigated the incompatibility phenotype of the mutants and found that both their styles and pollen behaved like those of the long-styled morph. There was a variation in the absolute length of styles, stamens and pollen size of the long-styled mutants. Furthermore, long-styled mutants possessing larger deletions tended to have their anthers and stigmas in closer proximity. We explored the inheritance of the S-locus mutations and found that only one of the deletion mutations was transmitted to progeny where we recovered seven such progeny. Remarkably, our data are consistent with the supergene model (GPA/gpa) of Primula. The long homostyle mutant appears to have deletions involving both the G and P loci. The other mutants appear to have deletions of the entire S-locus. The mutants generated will serve as a valuable resource for the molecular dissection of the S-locus region, and in the identification of genes determining distyly.

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“…The self-incompatibility reaction is triggered when the S gene is expressed in the pollen (SFB gene) and pistil (S-RNasa gene), which stops pollen tube growth. Knowledge of the genetic and molecular bases of the self-incompatible reaction has allowed the application of molecular techniques such as PCR amplification of S alleles with consensus or specific primers that amplify the two introns, which are part of the sequence of the S-RNasa gene (Tao et al, 1999;Tobutt et al, 2001;Wiersma et al, 2001;Sonneveld et al, 2001;Wünsch and Hormaza, 2004;Sonneveld et al, 2006), or amplify regions of the SFB gene (Sonneveld et al, 2005). The peach is self-fertile; in contrast, most cultivated Prunus species, such as cherry, almond, plums, and apricot, are self-infertile and therefore self-incompatible.…”

Section: Molecular Markersmentioning

confidence: 99%

Breeding rootstocks for Prunus species: Advances in genetic and genomics of peach and cherry as a model

Guajardo¹,

Hinrichsen

Muñoz

2015

Chilean J. Agric. Res.

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Prunus rootstock is an important choice in optimizing productivity of grafted cultivars. Nevertheless, many Prunus rootstocks are notoriously intolerant to hypoxia which is caused by waterlogging and/or heavy soils. There is no available information to help select Prunus rootstocks that are tolerant to stress conditions such as root hypoxia caused by excess moisture. Information from genetic maps has demonstrated a high level of synteny among Prunus species, and this suggests that they all share a similar genomic structure. It should be possible to identify the genetic determinants involved in tolerance to hypoxia and other traits in Prunus rootstocks by applying methods to identify regions of the genome involved in the expression of important traits; these have been developed mainly in peach which is the model species for the genus. Molecular markers that are tightly linked to major genes would be useful in marker-assisted selection (MAS) to optimize new rootstock selection. This article provides insight on the advances in the development of molecular markers, genetic maps, and gene identification in Prunus, mainly in peach; the aim is to provide a general approach for identifying the genetic determinants of hypoxia stress in rootstocks.

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Loss of Pollen-S Function in Two Self-Compatible Selections of Prunus avium Is Associated with Deletion/Mutation of an S Haplotype–Specific F-Box Gene

Cited by 238 publications

References 47 publications

Identification and characterization of S-RNase genes and S-genotypes in Prunus and Malus species

Identification and characterization of S-RNase genes and S-genotypes in Prunus and Malus species

Characterization of X-ray-generated floral mutants carrying deletions at the S-locus of distylous Turnera subulata

Breeding rootstocks for Prunus species: Advances in genetic and genomics of peach and cherry as a model

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