Loss of heterozygosity of Kras2 gene on 12p12-13 in Chinese colon carcinoma patients

Abstract

AIM:To study the loss of heterozygosity (LOH) on 12p12-13 in Chinese colon carcinoma patients.

Tumour maintenance is mediated by eNOS

“…In agreement, the wild-type counterpart of oncogenic Ras is not required for tumorigenesis ( Supplementary Fig. 15), and is even deleted in some tumours 21,22 ; whereas wild-type HRas and NRas are required for oncogenic KRasdriven tumour growth, and appear to have non-redundant activies [23][24][25][26] . Effects of eNOS on tumorigenesis have been largely attributed to its activity in endothelial cells 12 .…”

“…Indeed, GTP-bound endogenous wild-type HRas and NRas were reduced upon shRNA knockdown of eNOS. Moreover, as the wild-type allele of KRas is deleted in MIAPaCa-2 cells 20 , several oncogenic KRas-positive cell lines 20 and tumour tissues 21 , wild-type HRas and NRas, but not KRas, appear to be the targets of eNOS signalling in pancreatic cancer cells (Fig. 4c and Supplementary Fig.…”

Mutant allele specific imbalance in oncogenes with copy number alterations: Occurrence, mechanisms, and potential clinical implications

“…Prior to our discovery, LOH at chromosome 12p (where KRAS resides) had been reported to correlate with the presence of KRAS mutations in human lung cancer, CRC, PDAC, and prostate cancer [75; 76; 77; 78; 79; 80]. Loss of the WT Kras allele was also found associated with Kras activation in a spontaneous lung cancer mouse model [81; 82].…”