Loss of constitutional heterozygosity on chromosome 5q in hepatocellular carcinoma without cirrhosis

Ding, Shi-Fa; Na, Habib; Dooley, JS; Wood, C. B.; Bowles, L.; Delhanty, Jda

doi:10.1038/bjc.1991.468

Cited by 68 publications

(40 citation statements)

References 33 publications

(21 reference statements)

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“…The chromosomal regions deleted in his tumour were lq42-43 detected by the probe AMS32 and 5p by cMS621. These two probes also showed a high frequency of allele loss in HCC with liver cirrhosis, as previously reported (Ding et al, 1991). Patient No.…”

Section: Discussionsupporting

confidence: 62%

“…With the same method, we found a much higher frequency of allele loss in HCC (30/186, 16.1%) (Ding et al, 1991). For colorectal carcinomas, patients with a higher frequency of allelic losses had a considerably worse prognosis than did the other patients (Vogelstein et al, 1989).…”

Section: Discussionmentioning

confidence: 60%

“…The 18 RFLP probes for chromosomes 1,5,7,9,11,12,13,16,17 and 18 and the appropriate restriction enzymes are listed in Table II. These 18 probes were those used in the previous study on HCC (Ding et al, 1991), including probes screening regions near or flanking loci of most known tumour suppressor genes (Table II). If two alleles appeared as two separate bands in the resultant autoradiograph of the constitutional DNA, the patient was considered 'informative', or heterozygous, for the particular marker.…”

mentioning

confidence: 99%

“…Recently, we and others have studied the pattern of chromosome allele loss (loss of genetic material) in HCC (Ding et al, 1991;Zhang et al, 1990;Fujimori et al, 1991 DNA extraction and analysis DNA was prepared from blood and tissue samples by standard phenol/chloroform methods (Sambrook et al, 1989).…”

mentioning

confidence: 99%

“…Southern analyses were done as previously described (Ding et al, 1991). The 18 RFLP probes for chromosomes 1,5,7,9,11,12,13,16,17 and 18 and the appropriate restriction enzymes are listed in Table II.…”

mentioning

confidence: 99%

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Infrequent chromosome allele loss in fibrolamellar carcinoma

Ding

Delhanty²,

Bowles³

et al. 1993

Br J Cancer

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Summary As yet, there is no reported study of chromosome allele loss in fibrolamellar carcinoma (FLC), a distinct, rare variant of hepatocellular carcinoma (HCC). We searched for evidence of allele loss in FLC using 18 DNA probes for 10 chromosomes and compared the pattern of loss with our series of HCC. Two of the probes, AMS32 (lq42-43) and cMS621 (5p) showed allele losses in one tumour, while other probes showed no loss. The frequency of allele loss in FLC was much lower than in HCC, which may be associated with their different prognoses.

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Section: Discussionsupporting

confidence: 62%

Section: Discussionmentioning

confidence: 60%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Infrequent chromosome allele loss in fibrolamellar carcinoma

Ding

Delhanty²,

Bowles³

et al. 1993

Br J Cancer

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Frequent rearrangements at minisatellite loci D1S7 (1p33-35), D7S22 (7q36-ter) and D12S11 (12q24.3-ter) in hepatitis B virus-positive hepatocellular carcinomas from Thai patients

1997

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Primary hepatocellular carcinoma (HCC) is one of the most common cancers in Thailand; chronic infection with hepatitis B virus (HBV) is endemic and represents a major risk factor for the development of this cancer. Several mechanisms for HBV-related hepatocarcinogenesis have been proposed, among them a direct role of HBV in the promotion of genetic recombination leading to chromosomal alterations. Minisatellite DNA sequences are hypervariable regions dispersed throughout the genome which are susceptible to genetic recombination events. In the present study, somatic rearrangements affecting minisatellite sequences were examined in a total of 26 HCC from Thai patients. Multilocus DNA fingerprinting using probes 33.15 and 33.6 detected rearrangements in 11 and 12 HCC, respectively, all of them carrying integrated HBV DNA. The frequency of rearranged bands was calculated for each probe based on the total number of rearrangements observed in the 26 tumours and the total number of bands revealed by DNA fingerprinting in the non-tumour DNA. With each probe a total of 23 rearrangements was observed, yielding rearrangement frequencies of 3.7% and 4.2% for the 33.15 and 33.6 minisatellite families, respectively. To test for possible clustering of these rearrangements at specific loci, we used minisatellite locusspecific probes previously cloned from 33.15 and 33.6. Minisatellites located at 1p33-35, 7q36-ter and 12q24.3-ter were shown to be frequently affected by rearrangement events in this series of HBV-positive HCC. Frequent rearrangements at minisatellite locus D7S22 (7q36-ter) in HBV-positive human HCC have not been reported so far. Int.

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Distinct regions of frequent loss of heterozygosity of chromosome 5p and 5q in human esophageal cancer

Peralta

Casson

Wang³

et al. 1998

Int. J. Cancer

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Loss of heterozygosity (LOH) studies reported thus far suggest that tumor suppressor loci on chromosome 5q are important in esophageal cancer (EC) while little is known about the involvement of chromosome 5p. To investigate the potential existence of tumor suppressor gene(s) on chromosome 5 contributing to the development of EC, we performed LOH studies using a total of 24 polymorphic markers spanning the entire chromosome 5. Seventy primary esophageal cancers were microdissected and allelic deletions were detected by polymerase chain reaction (PCR)‐single strand conformation polymorphism or by microsatellite analysis. LOH was observed in at least 1 of the loci in 47 of 70 (67%) esophageal tumors. Initially, 40 tumors [24 squamous cell carcinomas (SCC) and 16 adenocarcinomas (ADC)], each with matched histologically normal esophageal mucosa, were analyzed at 15 marker loci on 5p and 5q. A novel locus, D5S667 on 5p15.2, exhibited the highest frequency of LOH (44%) in these tumors along with another previously reported region of frequent deletion, irf‐1 (5q31.1). In a series of 30 additional EC tumors (11 SCC and 19 ADC), a detailed LOH analysis of chromosome 5p15.2 region was conducted using 10 additional polymorphic markers, which mapped the frequently deleted region within 1 cM. Overall, LOH at the D5S667 locus was observed more frequently in SCC than in ADC (62% vs. 23%, p = 0.01). This significant rate of LOH of a distinct region of chromosome 5p implicates the existence of a putative tumor suppressor gene locus involved in EC. Int. J. Cancer 78:600–605, 1998. © 1998 Wiley‐Liss, Inc.

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Loss of constitutional heterozygosity on chromosome 5q in hepatocellular carcinoma without cirrhosis

Cited by 68 publications

References 33 publications

Infrequent chromosome allele loss in fibrolamellar carcinoma

Infrequent chromosome allele loss in fibrolamellar carcinoma

Frequent rearrangements at minisatellite loci D1S7 (1p33-35), D7S22 (7q36-ter) and D12S11 (12q24.3-ter) in hepatitis B virus-positive hepatocellular carcinomas from Thai patients

Distinct regions of frequent loss of heterozygosity of chromosome 5p and 5q in human esophageal cancer

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