Loops D, E and G in the <i>Drosophila</i> Dα1 subunit contribute to high neonicotinoid sensitivity of Dα1‐chicken β2 nicotinic acetylcholine receptor

Ihara, Makoto; Hikida, Mai; Matsushita, Hiroyuki; Yamanaka, Kyosuke; Kishimoto, Yuya; Kubo, Kazuki; Watanabe, Shun; Sakamoto, Mifumi; Matsui, Koutaro; Yamaguchi, Akihiro; Okuhara, Daiki; Furutani, Shogo; Sattelle, David B.; Matsuda, Kazuhiko

doi:10.1111/bph.13914

Cited by 23 publications

(24 citation statements)

References 51 publications

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“…The pcDNA3.1 plasmid containing the wild-type D. melanogaster nAChRα1 coding sequences ( nAChRα1 -pcDNA3.1) was synthesized previously described ( Ihara et al, 2018 ). Briefly, nAChRα1 -pcDNA3.1 was digested with EcoR I and Not I, and then the digested nAChRα1 fragment was ligated with a EcoR I- Not I–digested pWALIUM10-moe plasmid ( Perkins et al, 2015 ).…”

Section: Methodsmentioning

confidence: 99%

Neuronal octopamine signaling regulates mating-induced germline stem cell increase in female Drosophila melanogaster

Yoshinari

Ameku

Kondo

et al. 2020

eLife

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Stem cells fuel the development and maintenance of tissues. Many studies have addressed how local signals from neighboring niche cells regulate stem cell identity and their proliferative potential. However, the regulation of stem cells by tissue-extrinsic signals in response to environmental cues remains poorly understood. Here we report that efferent octopaminergic neurons projecting to the ovary are essential for germline stem cell (GSC) increase in response to mating in female Drosophila. The neuronal activity of the octopaminergic neurons is required for mating-induced GSC increase as they relay the mating signal from sex peptide receptor-positive cholinergic neurons. Octopamine and its receptor Oamb are also required for mating-induced GSC increase via intracellular Ca2+ signaling. Moreover, we identified Matrix metalloproteinase-2 as a downstream component of the octopamine-Ca2+ signaling to induce GSC increase. Our study provides a mechanism describing how neuronal system couples stem cell behavior to environmental cues through stem cell niche signaling.

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Section: Methodsmentioning

confidence: 99%

Neuronal octopamine signaling regulates mating-induced germline stem cell increase in female Drosophila melanogaster

Yoshinari

Ameku

Kondo

et al. 2020

eLife

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“…cRNA was dissolved in RNase free water at a concentration of 1 μg/ μL. To express (Dα1) 3 (β2) 2 and (Dα1) 2 (β2) 3 nAChRs in Xenopus oocytes, cRNAs of Dα1 and β2 subunits were mixed at ratios of 5:1 and 1:5, respectively 15, 16 .…”

Section: Methodsmentioning

confidence: 99%

“…After treatment with collagenase and the follicle cell layers were removed from oocytes 6,13,17,18 . Each oocyte was injected with 50 nL of the cRNA solution and incubated for 4 or 5 days at 16 °C in the standard oocyte saline (SOS, pH 7.6) supplemented with penicillin (100 units/mL), streptomycin (100 μg/mL), gentamycin (20 μg/mL) and sodium pyruvate (2.5 mM) at 16 °C 15, 16 . Each data was obtained using oocytes from at least two frogs.…”

Section: Methodsmentioning

confidence: 99%

“…The compound was bath-applied in SOS for 3-5 s at an interval of 3-5 min with increasing agonist concentrations 15,16 . When recording the peak amplitude of the responses to imidacloprid and thiacloprid at concentrations higher than 1 μM, one oocyte was used for recording one response and abandoned to prevent underestimation of agonist responses resulting from irreversible nAChR desensitization.…”

Section: Methodsmentioning

confidence: 99%

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The mechanism of loop C-neonicotinoid interactions at insect nicotinic acetylcholine receptor α1 subunit predicts resistance emergence in pests

Shimada

Kamiya

Shigetou

et al. 2020

Sci Rep

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Neonicotinoids selectively modulate insect nicotinic acetylcholine receptors (insect nAChRs). Studies have shown that serine with ability to form a hydrogen bond in loop C of some insect nAChR α subunits and glutamate with a negative charge at the corresponding position in vertebrate nAChRs may contribute to enhancing and reducing the neonicotinoid actions, respectively. However, there is no clear evidence what loop C properties underpin the target site actions of neonicotinoids. Thus, we have investigated the effects of S221A and S221Q mutations in loop C of the Drosophila melanogaster Dα1 subunit on the agonist activity of imidacloprid and thiacloprid for Dα1/chicken β2 nAChRs expressed in Xenopus laevis oocytes. The S221A mutation hardly affected either the affinity or efficacy for ACh and imidacloprid, whereas it only slightly reduced the efficacy for thiacloprid on the nAChRs with a higher composition ratio of β2 to Dα1 subunits. The S221Q mutation markedly reduced the efficacy of the neonicotinoids for the nAChRs with a higher composition of the β2 subunit lacking basic residues critical for binding neonicotinoids. Hence, we predict the possibility of enhanced neonicotinoid resistance in pest insect species by a mutation of the serine when it occurs in the R81T resistant populations lacking the basic residue in loop D of the β1 subunit.

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“…Molecular details of the interaction of neonicotinoids with Drosophila Dα1 subunit‐containing nAChRs were examined by computer modelling, mutagenesis and oocyte electrophysiology, by Ihara et al . ().…”

Section: Insect Nachrsmentioning

confidence: 97%

Nicotinic acetylcholine receptors

Wonnacott

Bermúdez

Millar

et al. 2018

British J Pharmacology

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Loops D, E and G in the Drosophila Dα1 subunit contribute to high neonicotinoid sensitivity of Dα1‐chicken β2 nicotinic acetylcholine receptor

Abstract: This article is part of a themed section on Nicotinic Acetylcholine Receptors. To view the other articles in this section visit http://onlinelibrary.wiley.com/doi/10.1111/bph.v175.11/issuetoc.

Cited by 23 publications

References 51 publications

Neuronal octopamine signaling regulates mating-induced germline stem cell increase in female Drosophila melanogaster

Neuronal octopamine signaling regulates mating-induced germline stem cell increase in female Drosophila melanogaster

The mechanism of loop C-neonicotinoid interactions at insect nicotinic acetylcholine receptor α1 subunit predicts resistance emergence in pests

Nicotinic acetylcholine receptors

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