Longitudinal [18F]FDG-PET/CT analysis of the glucose metabolism in ApoE-deficient mice

Abstract

Background Strong line of evidence suggests that the increased risk to develop AD may at least be partly mediated by cholesterol metabolism. A key regulator of cholesterol transport is the Apolipoprotein E4 (ApoE4), which plays a fundamental role in neuronal maintenance and repair. Impaired function of ApoE4 may contribute to altered cerebral metabolism leading to higher susceptibility to neurodegeneration. Methods To determine a possible link between ApoE function and alterations in AD in the brain of Apolipoprotein E-deficient mice (ApoE−/−) in a longitudinal manner metabolic and neurochemical parameters were analyzed. Cortical metabolism was measured by 2-deoxy-2-[18F]fluoroglucose ([18F]FDG)-PET/CT and proton magnetic resonance spectroscopy (1H-MRS) served to record neurochemical status. Results By using [18F]FDG-PET/CT, we showed that brain metabolism declined significantly stronger with age in ApoE−/− versus wild type (wt) mice. This difference was particularly evident at the age of 41 weeks in almost each analyzed brain region. In contrast, the 1H-MRS-measured N-acetylaspartate to creatine ratio, a marker of neuronal viability, did not decline with age and did not differ between ApoE−/− and wt mice. Conclusion In summary, this longitudinal in vivo study shows for the first time that ApoE−/− mice depict cerebral hypometabolism without neurochemical alterations.

Long-Term Caloric Restriction Attenuates β-Amyloid Neuropathology and Is Accompanied by Autophagy in APPswe/PS1delta9 Mice

“…PET/CT imaging and data analysis were performed according to previous works of our group [ 21 , 26 , 27 ] using PMOD software (version 3.7; PMOD Technologies LLC, Zürich, Switzerland). The processed PET images were subsequently co-registered with the mouse brain volume-of-interest (VOI) template (Mouse Mirrione atlas), and the PMOD software and tracer uptake values were extracted for each delineated VOI.…”

Rabbit systemic glucose metabolism map by total-body dynamic PET/CT technology

“…The excess visceral fat is related to abnormal glucose tolerance. In addition, Kuhla et al [25] reported that the ApoE can also effected glucose metabolism in brain. In this study all the animals are controlled the variables.…”