Long-term treatment with 9-cis-β-carotene rich alga Dunaliella bardawil ameliorates photoreceptor degeneration in a mouse model of retinoid cycle defect

Edelshtain, Victoria; Peled, Alon; Tzameret, Adi; Chen, Sarah Pri; Ziv, Hana; Derazne, Estela; Harats, Dror; Greenberg, Rachel G.; Harari, Ayelet; Shaish, Aviv; Sher, Ifat; Rotenstreich, Ygal

doi:10.1016/j.algal.2019.101607

Cited by 6 publications

(2 citation statements)

References 47 publications

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“…Mice were sacrificed at 13 weeks of age, and their eyes were fixed in 4% formaldehyde, as previously described [39,40]. Retinal paraffin sections were deparaffinized and rehydrated.…”

Section: Immunofluorescence Stainingmentioning

confidence: 99%

Intrinsic Expression of Coagulation Factors and Protease Activated Receptor 1 (PAR1) in Photoreceptors and Inner Retinal Layers

Goldberg

Sher

Qassim

et al. 2022

IJMS

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The aim of this study was to characterize the distribution of the thrombin receptor, protease activated receptor 1 (PAR1), in the neuroretina. Neuroretina samples of wild-type C57BL/6J and PAR1−/− mice were processed for indirect immunofluorescence and Western blot analysis. Reverse transcription quantitative real-time PCR (RT-qPCR) was used to determine mRNA expression of coagulation Factor X (FX), prothrombin (PT), and PAR1 in the isolated neuroretina. Thrombin activity following KCl depolarization was assessed in mouse neuroretinas ex vivo. PAR1 staining was observed in the retinal ganglion cells, inner nuclear layer cells, and photoreceptors in mouse retinal cross sections by indirect immunofluorescence. PAR1 co-localized with rhodopsin in rod outer segments but was not expressed in cone outer segments. Western blot analysis confirmed PAR1 expression in the neuroretina. Factor X, prothrombin, and PAR1 mRNA expression was detected in isolated neuroretinas. Thrombin activity was elevated by nearly four-fold in mouse neuroretinas following KCl depolarization (0.012 vs. 0.044 mu/mL, p = 0.0497). The intrinsic expression of coagulation factors in the isolated neuroretina together with a functional increase in thrombin activity following KCl depolarization may suggest a role for the PAR1/thrombin pathway in retinal function.

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“…Mice were sacrificed at 13 weeks of age, and their eyes were fixed in 4% formaldehyde, as previously described [39,40]. Retinal paraffin sections were deparaffinized and rehydrated.…”

Section: Immunofluorescence Stainingmentioning

confidence: 99%

Intrinsic Expression of Coagulation Factors and Protease Activated Receptor 1 (PAR1) in Photoreceptors and Inner Retinal Layers

Goldberg

Sher

Qassim

et al. 2022

IJMS

Self Cite

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“…Therefore, new pharmacological therapies are currently needed to mitigate the pro-inflammatory environment typically induced after subretinal administration of gene therapy, contributing to the prolonged survival of photoreceptors. Among the various pharmacotherapies available, some studies have emphasized the protective effects of natural polyphenols in slowing down the degeneration of cones in individuals with RP [53][54][55].…”

Section: Introductionmentioning

confidence: 99%

Piceid Octanoate Protects Retinal Cells against Oxidative Damage by Regulating the Sirtuin 1/Poly-ADP-Ribose Polymerase 1 Axis In Vitro and in rd10 Mice

Moshtaghion,

Caballano-Infantes,

Plaza Reyes

et al. 2024

Antioxidants

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Retinitis pigmentosa is a common cause of inherited blindness in adults, which in many cases is associated with an increase in the formation of reactive oxygen species (ROS) that induces DNA damage, triggering Poly-ADP-Ribose Polymerase 1 (PARP1) activation and leading to parthanatos-mediated cell death. Previous studies have shown that resveratrol (RSV) is a promising molecule that can mitigate PARP1 overactivity, but its low bioavailability is a limitation for medical use. This study examined the impact of a synthesized new acylated RSV prodrug, piceid octanoate (PIC-OCT), in the 661W cell line against H2O2 oxidative stress and in rd10 mice. PIC-OCT possesses a better ADME profile than RSV. In response to H2O2, 661W cells pretreated with PIC-OCT preserved cell viability in more than 38% of cells by significantly promoting SIRT1 nuclear translocation, preserving NAD+/NADH ratio, and suppressing intracellular ROS formation. These effects result from expressing antioxidant genes, maintaining mitochondrial function, reducing PARP1 nuclear expression, and preventing AIF nuclear translocation. In rd10 mice, PIC-OCT inhibited PAR-polymer formation, increased SIRT1 expression, significantly reduced TUNEL-positive cells in the retinal outer nuclear layer, preserved ERGs, and enhanced light chamber activity (all p values < 0.05). Our findings corroborate that PIC-OCT protects photoreceptors by modulating the SIRT1/PARP1 axis in models of retinal degeneration.

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Biorefinery potential of Dunaliella (Chlorophyta) for production of value‐added compounds

Sharma,

Singh,

Singh

2024

Biofuels Bioprod Bioref

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Dunaliella functions as a microscopic bioindustry that can capture atmospheric carbon dioxide and amass it into a range of economically viable compounds. Dunaliella‐based biorefineries have emerged as sustainable platforms for fostering a green economy and reducing carbon emissions. Dunaliella‐derived value‐added compounds are commercially important due to their application in the food, pharmaceutical, cosmeceutical, and nutraceutical sectors. The present study highlights the economic application of different Dunaliella species and the available sustainable technologies for the cultivation, harvesting, and extraction of bioactive compounds that have the potential for commercial scaleup. Their incorporation into biorefinery processes can reduce dependency on energy‐intensive and low‐yield processes.This review briefly discusses the technoeconomic analysis (TEA) of Dunaliella‐driven industrial approaches for the optimization of effective yield and determination of final production costs. The major algal biorefineries are operational in countries like Australia, China, India, Israel, Japan, Portugal, Spain and the USA. The authors have comprehensively deliberated different aspects of Dunaliella from physiological to industrial applications, meanwhile underscoring the existing knowledge gaps that impede the success of Dunaliella biorefineries. The current industrial methods being employed for biorefineries are limited to producing individual bioproducts, which may not be fully cost effective, making them less competitive in the global market. However, the analysis carried out can serve as a starting point and can guide stakeholders towards the future direction of Dunaliella‐based research and the circular bioeconomy.

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Long-term treatment with 9-cis-β-carotene rich alga Dunaliella bardawil ameliorates photoreceptor degeneration in a mouse model of retinoid cycle defect

Cited by 6 publications

References 47 publications

Intrinsic Expression of Coagulation Factors and Protease Activated Receptor 1 (PAR1) in Photoreceptors and Inner Retinal Layers

Intrinsic Expression of Coagulation Factors and Protease Activated Receptor 1 (PAR1) in Photoreceptors and Inner Retinal Layers

Piceid Octanoate Protects Retinal Cells against Oxidative Damage by Regulating the Sirtuin 1/Poly-ADP-Ribose Polymerase 1 Axis In Vitro and in rd10 Mice

Biorefinery potential of Dunaliella (Chlorophyta) for production of value‐added compounds

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