We present a variability study of the lowest-luminosity Seyfert 1 nucleus of the galaxy NGC 4395 based on photometric monitoring campaigns in 2017 and 2018. Using 22 ground-based and space telescopes, we monitored NGC 4395 with a ∼5 minute cadence during a period of 10 days and obtained light curves in the ultraviolet (UV), V , J, H, and K/Ks bands as well as narrow-band Hα. The rootmean-square (RMS) variability is ∼ 0.13 mag in the Swift-UVM2 and V filter light curves, decreasing down to ∼ 0.01 mag in the K filter. After correcting for the continuum contribution to the Hα narrowband, we measured the time lag of the Hα emission line with respect to the V -band continuum as 55 +27−31 to 122 +33 −67 min in 2017 and 49 +15 −14 to 83 +13 −14 min in 2018, depending on assumptions about the continuum variability amplitude in the Hα narrow-band. We obtained no reliable measurements for the continuum-to-continuum lag between UV and V bands and among near-IR bands, owing to the large flux uncertainty of UV observations and the limited time baseline. We determined the active galactic nucleus (AGN) monochromatic luminosity at 5100Å, λL λ = (5.75 ± 0.40) × 10 39 erg s −1 , after 2 subtracting the contribution of the nuclear star cluster. While the optical luminosity of NGC 4395 is two orders of magnitude lower than that of other reverberation-mapped AGNs, NGC 4395 follows the size-luminosity relation, albeit with an offset of 0.48 dex (≥ 2.5σ) from the previous best-fit relation of Bentz et al. (2013).