Long-term, in toto live imaging of cardiomyocyte behaviour during mouse ventricle chamber formation at single-cell resolution

Yue, Yanzhu; Zong, Weijian; Li, Xin; Li, Jinghang; Zhang, Youdong; Wu, Runlong; Liu, Yazui; Cui, Jiahao; Wang, Qianhao; Bian, Yunkun; Yu, Xianhong; Liu, Yao; Tan, Guangming; Zhang, Yunfeng; Zhao, Gang; Zhou, Bin; Chen, Liangyi; Xiao, Wenlei; Cheng, Heping; He, Aibin

doi:10.1038/s41556-020-0475-2

Cited by 42 publications

(43 citation statements)

References 34 publications

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“…Apparently, however, there are a lot of things that we need to know before understanding how cell proliferation is involved in complex morphogenesis of the ventricles. In addition to the EdU/BrdU incorporation assay and phosphorylated histone H3 staining, a live imaging technique was recently developed for the cell growth analysis in the early embryonic heart, which provided information of the cell cycle speed and frequency as well as cell division angles (Yue et al., 2020). Such a new method will be useful to precisely examine how normal and abnormal cell proliferation influences late‐stage cardiac morphogenesis.…”

Section: Discussionmentioning

confidence: 99%

A role of Hey2 transcription factor for right ventricle development through regulation of Tbx2‐Mycn pathway during cardiac morphogenesis

et al. 2021

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A basic helix‐loop‐helix transcription factor Hey2 is expressed in the ventricular myocardium and endocardium of mouse embryos, and Hey2 null mice die perinatally showing ventricular septal defect, dysplastic tricuspid valve and hypoplastic right ventricle. In order to understand region‐specific roles of Hey2 during cardiac morphogenesis, we generated Hey2 conditional knockout (cKO) mice using Mef2c‐AHF‐Cre, which was active in the anterior part of the second heart field and the right ventricle and outflow tract of the heart. Hey2 cKO neonates reproduced three anomalies commonly observed in Hey2 null mice. An earliest morphological defect was the lack of right ventricular extension along the apico‐basal axis at midgestational stages. Underdevelopment of the right ventricle was present in all cKO neonates including those without apparent atresia of right‐sided atrioventricular connection. RNA sequencing analysis of cKO embryos identified that the gene expression of a non‐chamber T‐box factor Tbx2 was ectopically induced in the chamber myocardium of the right ventricle. Consistently, mRNA expression of the Mycn transcription factor, which was a cell cycle regulator transcriptionally repressed by Tbx2, was down regulated, and the number of S‐phase cells was significantly decreased in the right ventricle of cKO heart. These results suggest that Hey2 plays an important role in right ventricle development during cardiac morphogenesis, at least in part, through mitigating Tbx2‐dependent inhibition of Mycn expression.

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Section: Discussionmentioning

confidence: 99%

A role of Hey2 transcription factor for right ventricle development through regulation of Tbx2‐Mycn pathway during cardiac morphogenesis

et al. 2021

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“…This would enable a direct assessment of non-cell-autonomous effects exerted by the neighboring cells on an individual cell or vice versa. So far, this method has mostly been used to visualize cell and collective migration behaviors in smaller in vivo systems such as, e.g., Drosophila (Krzic et al, 2012;Tomer et al, 2012), zebrafish (Nogare et al, 2017;Hiscock et al, 2018;Shah et al, 2019) and larger systems such as mouse gastrulation and heart tissue (Megason and Fraser, 2007;Stewart et al, 2009;McDole et al, 2018;Yue et al, 2020). In toto imaging mostly involves labeling of all cell membranes so each cell in the organism/microenvironment can be tracked and segmented (Nogare et al, 2017).…”

Section: In Toto Live-imagingmentioning

confidence: 99%

Non-Cell-Autonomous Mechanisms in Radial Projection Neuron Migration in the Developing Cerebral Cortex

Hansen

Hippenmeyer

2020

Front. Cell Dev. Biol.

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Concerted radial migration of newly born cortical projection neurons, from their birthplace to their final target lamina, is a key step in the assembly of the cerebral cortex. The cellular and molecular mechanisms regulating the specific sequential steps of radial neuronal migration in vivo are however still unclear, let alone the effects and interactions with the extracellular environment. In any in vivo context, cells will always be exposed to a complex extracellular environment consisting of (1) secreted factors acting as potential signaling cues, (2) the extracellular matrix, and (3) other cells providing cell-cell interaction through receptors and/or direct physical stimuli. Most studies so far have described and focused mainly on intrinsic cell-autonomous gene functions in neuronal migration but there is accumulating evidence that noncell-autonomous-, local-, systemic-, and/or whole tissue-wide effects substantially contribute to the regulation of radial neuronal migration. These non-cell-autonomous effects may differentially affect cortical neuron migration in distinct cellular environments. However, the cellular and molecular natures of such non-cell-autonomous mechanisms are mostly unknown. Furthermore, physical forces due to collective migration and/or community effects (i.e., interactions with surrounding cells) may play important roles in neocortical projection neuron migration. In this concise review, we first outline distinct models of non-cell-autonomous interactions of cortical projection neurons along their radial migration trajectory during development. We then summarize experimental assays and platforms that can be utilized to visualize and potentially probe non-cell-autonomous mechanisms. Lastly, we define key questions to address in the future.

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“…Morphology changes of Drosophila embryos at different biological stages, such as germ band retraction, segment formation, and dorsal closure, were imaged with a LSFM [73]. Cardiomyocytes were imaged based on a customized LSFM for up to 1.5 days to investigate the holistic cell behaviors sculpting the four-chambered mammalian heart [74]. It should be noted that sampled images of LSFM are invulnerable to out-of-focus blurs, and they can exhibit more detailed features than normal wide-field microscopes [75].…”

Section: Light Sheet Fluorescence Microscope (Lsfm)mentioning

confidence: 99%

Advanced Biological Imaging for Intracellular Micromanipulation: Methods and Applications

et al. 2020

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Intracellular micromanipulation assisted by robotic systems has valuable applications in biomedical research, such as genetic diagnosis and genome-editing tasks. However, current studies suffer from a low success rate and a large operation damage because of insufficient information on the operation information of targeted specimens. The complexity of the intracellular environment causes difficulties in visualizing manipulation tools and specimens. This review summarizes and analyzes the current development of advanced biological imaging sampling and computational processing methods in intracellular micromanipulation applications. It also discusses the related limitations and future extension, providing an important reference about this field.

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Long-term, in toto live imaging of cardiomyocyte behaviour during mouse ventricle chamber formation at single-cell resolution

Cited by 42 publications

References 34 publications

A role of Hey2 transcription factor for right ventricle development through regulation of Tbx2‐Mycn pathway during cardiac morphogenesis

A role of Hey2 transcription factor for right ventricle development through regulation of Tbx2‐Mycn pathway during cardiac morphogenesis

Non-Cell-Autonomous Mechanisms in Radial Projection Neuron Migration in the Developing Cerebral Cortex

Advanced Biological Imaging for Intracellular Micromanipulation: Methods and Applications

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