Long-term impact of fecal transplantation in healthy volunteers

Goloshchapov, Oleg V.; Olekhnovich, Evgenii I.; Сидоренко, С В; Moiseev, Ivan S.; Kucher, Maxim A.; Fedorov, Dmitry E.; Pavlenko, Alexander V.; Manolov, Alexander I.; Gostev, Vladimir; Veselovsky, Vladimir A.; Klimina, Ksenia M.; Kostryukova, Elena S.; Bakin, Evgeny A.; Shvetcov, Alexander N.; Гумбатова, Э. Д.; Klementeva, Ruslana V.; Щербаков, А. А.; Горчакова, М. В.; Egozcue, Juan José; Pawlowsky‐Glahn, Vera; Suvorova, Maria A.; Чухловин, А. Б.; Govorun, Vadim M.; Ilina, Elena; Afanasyev, Boris

doi:10.1101/671644

Cited by 3 publications

(3 citation statements)

References 65 publications

(68 reference statements)

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“…2, however the various researchers of this discipline, give the alteration of the intestinal microbiome a primary role on the state of health or disease of patients [12]. Starting from these assumptions, the use of faeces transplantation containing particular microbial strains, to rebalance and correct intestinal dysbiosis, is even postulated [13][14][15]. This method has been the subject of many ethical and scientific criticisms.…”

Section: Diet Genetic Polymorphisms and Pathologiesmentioning

confidence: 99%

From Nutrigenomics to Microbiomics: New Interpretations

Riccardi¹,

Resta²,

Fabbroni³

2021

J Biotechnol Bioinforma Res

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In this work we analyze the current interpretations based on the studies of Nutrigenomic and Microbiomics, according to which, at the base of many pathological and dysmetabolic manifestations, there would be or variations of single nucleotide polymorphisms (Snps), in the Nutrigenomic vision, or changes of the normal gut microbiota, in the Microbiomic conception. In particular, we analyze critically, the singular pathogenic coincidence, which for the same pathologies calls into question or Snps mutations or the presence of specific modifications of the Microbiota. Other possible causes of observed diseases are thus neglected. On the basis of the literature examined, we present a different interpretation of pathogenic causes.

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Section: Diet Genetic Polymorphisms and Pathologiesmentioning

confidence: 99%

From Nutrigenomics to Microbiomics: New Interpretations

Riccardi¹,

Resta²,

Fabbroni³

2021

J Biotechnol Bioinforma Res

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“…Th e same fecal DNA samples were used for detailed 16S rDNA sequencing by means of NGS technique, as elsewhere described [11]. Th e serial microbiome sequencing procedure was carried out with Illumina™ HiSeq 2500 system.…”

Section: Laboratory Screening Of Gut Microbiotamentioning

confidence: 99%

Bacteroides fragilis is a potential marker of effective microbiota transplantation in acute graft-versus-host disease treatment

Goloshchapov

Bakin

Kucher

et al. 2020

CTT

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Summary Fecal microbiota transplantation (FMT), as any other medical procedure, requires standardization of results, approaches, monitoring of its dynamics and microbiota engraftment evaluation. The aim of the present study was to compare efficiency and results of PCR and 16S RNA-based sequencing in order to trace the dynamics of microbiota composition after FMT. Patients and methods The prospective, single-center study included 27 patients with acute intestinal and chronic (overlap syndrome) graft-versus-host disease (GvHD) developed after allogeneic hematopoietic stem cell transplantation (HSCT). FMT in 19 cases was performed, mostly, with ingestible capsules, eight placebo-treated patients were included into control group. Quantitative changes of different bacterial groups in fecal microbiota were assessed by means of real-time multiplex PCR, being compared with16S rRNA sequencing technique at the terms of D+3, D+16, D+30, D+60 and D+120 following FMT. Clinical response was determined by 4 scales evaluating intestinal syndrome and GvHD grade. Results When evaluating stool consistence according to Bristol scale as an index of GvHD therapy efficiency, we have observed complete clinical response by the D+120 after FMT in nine cases (47% with Bristol score of ≤4 points), and nine patients (47%) showed improved stool properties (>4 points). In the placebo group, complete or partial response was revealed, respectively, in one (13%), and four cases (50%) on the D+120. Multiplex PCR of fecal microbiota has shown a different time course in FMT- and placebo-treated patients, when compared to their initial (pre-FMT) levels. Total bacterial mass and copy numbers of distinct microbial species exhibited sufficient increase after FMT. Such shifts were demonstrable on D+30 for total microbial mass (p=0.002); Escherichia coli (p=0.001); Bacteroides fragilis group (p=0.05); Faecalibacterium prausnitzii (p=0.005). Meanwhile, the numbers Lactobacillus spp., and Bacteroides thetaiotaomicron, generally, were not changed over this time period. Moreover, in the control group (placebo) we have not found significant fecal microbiota changes against initial levels during 120 days monitoring period. Over 120 days of observation, we have also found some differences of the microbiota dynamics for the subgroups with complete response and partial/no response: Bifidobacterium spp. (р<0.047), E.coli (р<0.00047), B. fragilis group (p=5.6×10-5), F.prausnitzii (р<0.0062). Conclusions 1. Quantitative PCR of the major bacterial groups of gut microbiota, e.g., Bifidobacterium spp., E. coli, B. fragilis group, F. prausnitzii could be used as microbiological markers for evaluation of changing microbial landscape after FMT as a routine molecular biology technique. 2. The genocopy counts of B. fragilis group correlate with clinical response in the patients with intestinal GvHD after HSCT, either with, or without FMT procedure.

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“…Microbial fecal transplantation is preferable in this case, which has proven effect in treating pseudomembranous colitis, ulcerative colitis, multiple sclerosis, Parkinson's disease and other diseases. 6,[21][22][23] Efforts are also under way to create artificial microbiota by administering various donor strains isolated from the intestines of healthy donors, 23,24 as well as autologous strains or consortia grown from the patient's own fecal samples collected before onset of the disease and its treatment. 1 Switch from proteolytic to saccharolytic fermentation could be of major interest for the prevention and/or treatment of metabolic diseases.…”

Section: Introductionmentioning

confidence: 99%

Untitled

2021

GHOA

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Fecal transplantation and the introduction of indigenous bacteria (autoprobiotics) are currently in demand for the treatment of many diseases and accompanied by the development of intestinal dysbiosis and impairment of its functions. The specific effects of autologous (autoprobiotic) fecal microbiota transplants (AFMT) and a autoprobiotic consortium of indigenous fecal bacteria (AC) on the restoration of intestinal microbiota and activity of key digestive enzymes in Wistar rats with experimental dysbiosis was studied.The composition of the fecal microbiome after correction of dysbiosis induced by ampicillin and metronidazole in Wistar rats of using AFMT (group F) and AC (group A) so as after introduction of phosphate buffer saline (group C1) was studied by metagenome analysis (16S rRNA) and qPCR comparing with normal microbiota (group C2, without consumption). The activity of alkaline phosphatase (AP), maltase (ML), and aminopeptidase N (APN) in the chyme of various intestinal parts was evaluated using biochemical methods.The most significant changes of microbiota composition were observed in group C1: the representation of genera Lactobacillus, Ruminococcus and Prevotella spp. decreased, Proteobacteria, Families Enterobacteriaceae, genera Proteus spp., Klebsiella Enterobacter, Suterella, Serratia increased. The microbiome of animals from group F was characterized by a sharp increase in the representation of the phylum Bacteroidetes, genera Escherichia coli and Suterella sp., against the background of a decrease of phylum Firmicutes relative abundance. The representation of Lactobacillus spp., Enterococcus spp., Propionibacterium spp., Bifidobacterium spp. and Proteus spp. and E.coli), increased after the introduction of AC. Despite more pronounced residual changes in intestinal microbiome of rats from group C1, no significant changes in the activity of key digestive enzymes were detected. The exception was low AP activity in the duodenum chyme in group C1, possibly indicating insufficient protection from lipopolysaccharide of gram-negative bacteria. AP was higher in group A than in group C2 in the chyme of the colon. AMFT induced more sufficient changes in the carbohydrate and protein metabolism: the increase ML in small intestine and reduced of APN in the chyme in the virtually throughout the intestine.Individual representatives of intestinal microbiota may affect the activity of digestive enzymes. This study has revealed the presence of relationships between the activity of ML in the chyme and the representation of Bacteroides spp. (direct correlation) and Prevotella spp. (inverse correlation). It is necessary to take into account the specific influence of AMFT and AC on microbiota and digestive function for choosing a means for the correction of intestinal dysbiosis.

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Long-term impact of fecal transplantation in healthy volunteers

Cited by 3 publications

References 65 publications

From Nutrigenomics to Microbiomics: New Interpretations

From Nutrigenomics to Microbiomics: New Interpretations

Bacteroides fragilis is a potential marker of effective microbiota transplantation in acute graft-versus-host disease treatment

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