Long-term follow-up of the Ionescu-Shiley mitral pericardial xenograft

Gabbay, Shlomo; Bortolotti, Uberto; Wasserman, Fred; Tindel, Nathaniel L.; Factor, Stephen M.; Frater, Robert W.M.

doi:10.1016/s0022-5223(19)35444-3

Cited by 63 publications

(10 citation statements)

References 20 publications

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“…Biological tissues have been used clinically as heart valve prostheses, small‐diameter vascular grafts, and pericardial substitutes 17–19. However, these biological tissues have to be fixed with a crosslinking agent before they can be implanted in humans 1.…”

Section: Discussionmentioning

confidence: 99%

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Stability of a biological tissue fixed with a naturally occurring crosslinking agent (genipin)

Sung

Liang

Chen

et al. 2001

J. Biomed. Mater. Res.

204

149

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The study was undertaken to investigate the stability of a biological tissue fixed with a naturally occurring crosslinking agent (genipin) at distinct elapsed storage durations. The glutaraldehyde-fixed counterpart was used as a control. Porcine pericardia procured from a slaughterhouse were used as raw materials. After fixation, the fixed tissues were sterilized in a graded series of ethanol solutions and thoroughly rinsed in phosphate buffered saline for 1 day, and then stored in a jar containing sterilized water. The samples were taken out and tested for their stability during the durations of 1day through 6 months after storage. The stability of each study group was tested by measuring its tensile strength, free-amino-group content, and denaturation temperature. Additionally, the cytotoxicity of each test sample and its corresponding storage solution were investigated in vitro using 3T3 fibroblasts. The results were examined using a microscope and 3-(4,5-dimethylthiazol-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. It was found that the stability of the genipin-fixed tissue during storage was superior to its glutaraldehyde-fixed counterpart. The differences in stability between the genipin- and glutaraldehyde-fixed tissues during storage may be caused by their differences in crosslinking structure. There was no apparent cytotoxicity for both the genipin-fixed tissue and its corresponding storage solution throughout the entire course of the study, whereas significant cytotoxicity was observed for both the glutaraldehyde-fixed tissue and its storage solution. However, the cytotoxicity of the glutaraldehyde-fixed tissue decreased with increasing elapsed storage duration, whereas that of its corresponding storage solution increased. This suggested that the toxic residues remaining in the glutaraldehyde-fixed tissue leached out slowly into its corresponding storage solution during the course of storage.

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Section: Discussionmentioning

confidence: 99%

“…It is speculated that it may take a longer storage duration before this phenomenon can be observed. Tissue failure of long‐term implants has been reported in the literature 1, 17. The reversibility of glutaraldehyde crosslinking is suggested as one of the reasons for the failure of long‐term bioprosthetic implants 17, 26…”

Section: Discussionmentioning

confidence: 99%

Stability of a biological tissue fixed with a naturally occurring crosslinking agent (genipin)

Sung

Liang

Chen

et al. 2001

J. Biomed. Mater. Res.

204

149

View full text Add to dashboard Cite

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“…Biomeshes based on decellularized bovine pericardium (DBP) are widely used in reconstructive surgery by virtue of their ready availability and attractive biomechanical properties (Baharuddin et al, 2002;Colombo et al, 2009;Guerette et al, 2009;Limpert et al, 2009;Schlee et al, 2012;Mallis et al, 2017). They are already being applied to intracardiac surgery (Gabbay et al, 1984), abdominal wall repair (Limpert et al, 2009), treatment of gingival recession (Schlee et al, 2012), bone and periodontal defects (Stavropoulos et al, 2011), duraplastics (Baharuddin et al, 2002), ophthalmology (Gupta et al, 2002;Koay et al, 2008;Quaranta et al, 2013), the repair of rectovaginal septum defects (Guerette et al, 2009), as dental membranes and venous stent covers (Colombo et al, 2009). However, the xenogenic nature of the biomesh source prompts an immune response in the human body and facile biodegradation.…”

Section: Introductionmentioning

confidence: 99%

Multiparametric Optical Bioimaging Reveals the Fate of Epoxy Crosslinked Biomeshes in the Mouse Subcutaneous Implantation Model

Elagin

Kuznetsova

Grebenik

et al. 2020

Front. Bioeng. Biotechnol.

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“…A particular interest is drawn to the pericardium tissue mostly due to its accessibility, acceptable biocompatibility and composition. Up to the date, it has been extensively used for various clinical applications including intracardiac surgery, abdominal wall repair, treatment of gingival recession, bone and periodontal defects, duraplastics, and in ophthalmology . The majority of the research use xenogenic tissues due to the ethical and safety concerns associated with allo‐ and autoplastics, respectively.…”

Section: Introductionmentioning

confidence: 99%

Chemical cross‐linking of xenopericardial biomeshes: A bottom‐up study of structural and functional correlations

Grebenik

Истранов

Истранова

et al. 2019

Xenotransplantation

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Decellularized bovine pericardium (DBP)-based biomeshes are the gold standard in reconstructive surgery. In order to prolong their stability after the transplantation, various chemical cross-linking strategies are employed. However, structural and functional properties of the biomeshes differ in dependence on the cross-linker used. Here, we performed a bottom-up study of structural and functional alterations of DBP-based biomeshes following cross-linking with hexamethylene diisocyanate (HMDC), ethylene glycol diglycidyl ether (EGDE), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and genipin. The in vitro cytotoxicity tests supported their clinical applicability. Their structural differences (eg roughness, fibre thickness, pore morphology) were evaluated using the two-photon confocal laser scanning, atomic force, scanning electron and polarized light microscopies. HMDC and EDC samples appeared to be the roughest. Complex mechanical trials indicated the tendency to reduced Young's Modulus and mechanical anisotropy values of DBP upon cross-linking. The lowest mechanical anisotropy was found in EDC and genipin sample groups.In vitro collagenase susceptibility was the highest for EDC samples and the lowest for EGDE samples. The comparative analysis of the results allowed us to recognize the strengths and weaknesses of each cross-linker in relation to a particular clinical application. K E Y W O R D Sbiomechanics, bovine pericardium, cross-linking, decellularization, reconstructive surgery

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Long-term follow-up of the Ionescu-Shiley mitral pericardial xenograft

Cited by 63 publications

References 20 publications

Stability of a biological tissue fixed with a naturally occurring crosslinking agent (genipin)

Stability of a biological tissue fixed with a naturally occurring crosslinking agent (genipin)

Multiparametric Optical Bioimaging Reveals the Fate of Epoxy Crosslinked Biomeshes in the Mouse Subcutaneous Implantation Model

Chemical cross‐linking of xenopericardial biomeshes: A bottom‐up study of structural and functional correlations

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