In this study, 20 Albino mice of the same daily age were used. All the animals were restrained in well-ventilated 50 ml tubes (plus 3 or 4 cm long middle tubes), which were slipped over the tail to restrict movement even more and left undisturbed under an opaque duration of the stress (30 min). After stress procedure was applied in all animals, 20 mice which have same alive weight average and constitute the study material were divided into two groups as control group (CG) (n=10) and study groups (SG) (n=10) for a 21-day treatment period equally and randomly. Normal tap water was added to the CG mice’ drinkers, while the hot spring water that was brought from the source each day as fresh added to SG mice’ drinkers and they were allowed to reach ad libitum. Clinical, hematological and blood biochemical parameters were measures on 1, 7, 14 and 21 days after treatment in all the animals. The results of this study showed that cortisol and glucose levels decreased in stressed mice treated with Süreyya I Hot Spring Water, while ALB, TP, T3 and T4 levels increased and these changes were statistically significant plessthan0.06) when compared to the control group.