Long–term culture of primary human lymphoblastic leukemia cells in the absence of serum or hematopoietic growth factors

Cell Cycle–Dependent Mechanisms Underlie Vincristine-Induced Death of Primary Acute Lymphoblastic Leukemia Cells

“…ALL cell cultures were maintained in suspension as described [25] in Iscove's modified Dulbecco's medium (IMDM) containing serum-free supplement (10 µg/mL cholesterol, 6 mg/mL human serum albumin, 0.5 µg/mL amphotericin, 1 µg/mL insulin, 200 µg/mL human apo-transferrin, 50 µM 2-mercaptoethanol, 2 mM glutamine and 50 units/mL penicillin). Cells were maintained at 37°C and 5% CO 2 .…”

“…Cells were maintained at 37°C and 5% CO 2 . The ALL cultures utilized, namely ALL-2 and ALL-5, correspond to their original designations of CM and PH cells, respectively [25]. Doubling time was determined by monitoring viable cell count daily for up to six days.…”

“…Among these were primary adult acute lymphoblastic leukemia (ALL) cells derived from several individual adult patients, expanded and established in culture using a defined serum-free medium [25,26]. In the present study, to determine whether cells in different cell cycle contexts were differentially affected by vincristine, we used centrifugal elutriation to isolate populations of ALL cells in different cell cycle phases.…”

p185BCR/ABL has a lower sensitivity than p210BCR/ABL to the allosteric inhibitor GNF-2 in Philadelphia chromosome-positive acute lymphatic leukemia

“…The PD-LTC were directly derived from bone marrow cells of Ph + ALL patients cultured in a specific culture medium. 13 We compared the responses from increasing concentrations of PD-LTC of two Ph + ALL patients expressing p185 BCR/ABL (BV and PH) with those of two patients expressing p210 BCR/ABL (CM and VB). As negative controls, we used the PD-LTC of a Ph-ALL patient (HP).…”

A mechanistic rationale for combining alemtuzumab and rituximab in the treatment of ALL

“…As donor cells, the leukemic CD20 ϩ CD52 ϩ ALL-CM cell line, previously established from patient 15, was used. 30 To assess uptake of antibody from the surface of ALL cells by monocytes, monocytes were stained with the membrane dye PKH-26 according to the manufacturer's instructions (Sigma-Aldrich). ALL-CM cells were then coated with 40 g/mL FITC-conjugated RTX (RTX-FITC) or ALM (ALM-FITC) for 30 minutes, washed, and plated in glass-bottomed Petri dishes (MatTec) in the 37°C thermostatic sample tray of a TCS-SP2 confocal laser scanning microscope with an inverted microscope stand DM-IRBE (both Leica Microsystems).…”