1979
DOI: 10.1073/pnas.76.10.5217
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Abstract: Capillary endothelial cells from rats, calves, and humans, have been carried in long-term culture. Bovine capillary endothelial cells have been cloned and maintained by serial passage for longer than 8 months. This prolonged culture was accomplished by using tumor-conditioned medium, gelatin-coated plates, and a method of enriching cells However, long-term culture and cloning of capillary endothelial cells have not previously been possible. Del Vecchio et al. (5) reported the isolation of capillary endotheli… Show more

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Cited by 678 publications
(304 citation statements)
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“…Angiogenesis involves proliferation of endothelial cells, chemotaxis and enzymatic degradation of the basement membrane of local blood vessels [8,12,33]. This process is essential in tissue growth and regeneration.…”
Section: Introductionmentioning
confidence: 99%
“…Angiogenesis involves proliferation of endothelial cells, chemotaxis and enzymatic degradation of the basement membrane of local blood vessels [8,12,33]. This process is essential in tissue growth and regeneration.…”
Section: Introductionmentioning
confidence: 99%
“…Bovine capillary endothelial (BCE) cells were isolated from the adrenal cortex of recently slaughtered yearling cattle by the method of Folkman et al (14). Cells were grown to confluence in a minimal essential medium (aMEM) containing 10% (vol/vol) calf serum and supplemented with medium conditioned by mouse sarcoma 180 cells as described (15).…”
Section: Methodsmentioning
confidence: 99%
“…Classic capillary endothelial cell isolation procedures involved separating the microvasculature from tissue and then allowing cells to grow from the vessels (20). Other investigators have isolated microvascular endothelial cells on the basis of induced expression of cell surface activation markers (21).…”
mentioning
confidence: 99%