Long-term and zero-order release of basic fibroblast growth factor from heparin-conjugated poly(l-lactide-co-glycolide) nanospheres and fibrin gel

Jeon, Oju; Kang, Sun-Woong; Lim, Hee-Won; Chung, Ji Hyung; Kim, Byung Soo

doi:10.1016/j.biomaterials.2005.08.030

Cited by 164 publications

(123 citation statements)

References 25 publications

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“…A study in rabbits using MSCs, elastic block copolymer scaffolds and TGF-β 3 , showed that chondroitin sulphate-bound TGF-β 3 had a slower release profile than TGF-β directly incorporated into the scaffold (Park et al, 2010). Similar results have been achieved by binding TGF-β 3 (Park et al, 2008) or bFGF (Jeon et al, 2006) to heparin. Loaded structures.…”

Section: Direct Attachment or Incorporationmentioning

confidence: 80%

Joint Cartilage Tissue Engineering and Pre-Clinical Safety and Efficacy Testing

Koch¹,

Moroni²,

Leysi-Derilou³

et al. 2011

Tissue Engineering for Tissue and Organ Regeneration

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Section: Direct Attachment or Incorporationmentioning

confidence: 80%

Joint Cartilage Tissue Engineering and Pre-Clinical Safety and Efficacy Testing

Koch¹,

Moroni²,

Leysi-Derilou³

et al. 2011

Tissue Engineering for Tissue and Organ Regeneration

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“…For example, heparin-conjugated PLGA nanospheres (HCPNs), trapped in cross-linked fibrin gel, were developed for long-term and zero-order delivery of bFGF. Heparin was immobilized on PLGA nanospheres via a coupling reaction in the presence of EDC and the bFGF was physically bonded to heparin [202]. The release kinetics of bFGF from HCPNs suspended in fibrin gel was of zero order, sustained for three weeks with no initial burst effect.…”

Section: Interaction With Glycosaminoglycansmentioning

confidence: 99%

Strategies for the chemical and biological functionalization of scaffolds for cardiac tissue engineering: a review

Tallawi

Rosellini

Barbani

et al. 2015

J. R. Soc. Interface.

288

208

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The development of biomaterials for cardiac tissue engineering (CTE) is challenging, primarily owing to the requirement of achieving a surface with favourable characteristics that enhances cell attachment and maturation. The biomaterial surface plays a crucial role as it forms the interface between the scaffold (or cardiac patch) and the cells. In the field of CTE, synthetic polymers ( polyglycerol sebacate, polyethylene glycol, polyglycolic acid, poly-L-lactide, polyvinyl alcohol, polycaprolactone, polyurethanes and poly(N-isopropylacrylamide)) have been proven to exhibit suitable biodegradable and mechanical properties. Despite the fact that they show the required biocompatible behaviour, most synthetic polymers exhibit poor cell attachment capability. These synthetic polymers are mostly hydrophobic and lack cell recognition sites, limiting their application. Therefore, biofunctionalization of these biomaterials to enhance cell attachment and cell material interaction is being widely investigated. There are numerous approaches for functionalizing a material, which can be classified as mechanical, physical, chemical and biological. In this review, recent studies reported in the literature to functionalize scaffolds in the context of CTE, are discussed. Surface, morphological, chemical and biological modifications are introduced and the results of novel promising strategies and techniques are discussed.

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“…21 For example, bone healing time varies from days to weeks to months, depending on the nature of bone defects. 22,23 Extensive efforts have been focused on reducing the initial burst release of drug carriers by controlling formulation parameters of the carriers such as molecular weight, hydrophilicity, as well as drug loading amount. 21 Recently, our laboratory has developed a biodegradable hydrogel of PEG sebacic acid diacrylate (PEGSDA), based on PEG and sebacic acid macromer terminated with acrylate groups.…”

Section: Introductionmentioning

confidence: 99%

Potential of Hydrogels Based on Poly(Ethylene Glycol) and Sebacic Acid as Orthopedic Tissue Engineering Scaffolds

Kim

Hefferan

Yaszemski

et al. 2009

Tissue Engineering Part A

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In this study, the bioactive effects of poly(ethylene glycol) (PEG) sebacic acid diacrylate (PEGSDA) hydrogels with or without RGD peptide modification on osteogenic differentiation and mineralization of marrow stromal cells (MSCs) were examined. In a separate experiment, the ability of PEGSDA hydrogel to serve as a delivery vehicle for bone morphogenetic protein 2 (BMP-2) was also investigated. As a scaffold, the attachment and proliferation of MSCs on PEGSDA hydrogel scaffolds with and without RGD peptide modification was similar to the control, tissue culture polystyrene. In contrast, cells were barely seen on unmodified PEG diacrylate (PEGDA) hydrogel throughout the culture period for up to 21 days. Osteogenic phenotypic expression such as alkaline phosphatase (ALP) of MSCs as well as mineralized calcium content were significantly higher on PEGSDA-based hydrogels than those on the control or PEGDA hydrogels. Potential use of PEGSDA scaffold as a delivery vehicle of osteogenic molecules such as BMP-2 was also evaluated. Initial burst release of BMP-2 from PEGSDA hydrogel scaffold (14.7%) was significantly reduced compared to PEGDA hydrogel scaffold (84.2%) during the first 3 days of a 21-day release period. ALP activity of an osteoblast was significantly higher in the presence of BMP-2 released from PEGSDA hydrogel scaffolds compared to that in the presence of BMP-2 released from PEGDA scaffolds, especially after 6 days of release. Overall, PEGSDA hydrogel scaffolds without further modification may be useful as orthopedic tissue engineering scaffolds as well as local drug carriers for prolonged sustained release of osteoinductive molecules.

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Long-term and zero-order release of basic fibroblast growth factor from heparin-conjugated poly(l-lactide-co-glycolide) nanospheres and fibrin gel

Cited by 164 publications

References 25 publications

Joint Cartilage Tissue Engineering and Pre-Clinical Safety and Efficacy Testing

Joint Cartilage Tissue Engineering and Pre-Clinical Safety and Efficacy Testing

Strategies for the chemical and biological functionalization of scaffolds for cardiac tissue engineering: a review

Potential of Hydrogels Based on Poly(Ethylene Glycol) and Sebacic Acid as Orthopedic Tissue Engineering Scaffolds

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