Long‐Range Fluorescence Propagation in Amyloidogenic β‐Sheet Films and Fibers

Apter, Boris; Fainberg, B. D.; Handelman, Amir; Lapsker, I.; Accardo, Antonella; Diaferia, Carlo; Morelli, Giancarlo; Rosenman, G.

doi:10.1002/adom.202000056

Cited by 21 publications

(36 citation statements)

References 58 publications

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“…This FL spectrum completely covers the OA spectrum of these amyloid-like fibers. Despite the full overlap of FL and OA spectra with a high OA coefficient, the FL light propagates in the PEG-F6 fibers for an unexpectedly long range of hundreds of micrometers, exceeding the length determined by classical light absorption by three to six times the corresponding wavelength throughout the visible region ( Figure 13) [133,134]. Such anomalous FL transmission indicates an exceptional physical mechanism guiding the long-range FL propagation.…”

Section: Physical Mechanism Of Fl In Amyloid and Amyloidogenic Fibrilsmentioning

confidence: 96%

“…The FL of the PEG-F6 amyloidogenic fibers exhibits a unique continuous spectrum occupying the entire visible region, owing to the additional strong green FL [95,133,134]. This FL spectrum completely covers the OA spectrum of these amyloid-like fibers.…”

Section: Physical Mechanism Of Fl In Amyloid and Amyloidogenic Fibrilsmentioning

confidence: 96%

“…Two-column images show passive optical waveguiding for three laser beams with wavelengths (from the top to the bottom) of 635, 520, and 450 nm in the PEG12-F6 peptide film. Optical waveguides and diffractive gratings for light beam coupling and decoupling were fabricated by Focus Ion Beam [133].…”

Section: Fluorescence In Pegylated Films and Fibersmentioning

confidence: 99%

“…The intriguing optoelectronic properties of PEGylated-F6 peptides indicate their promising wide applications in precision nanomedicine and integrated biooptics. In this context, recently, Apter et al tried to clarify the photonic effects in new bioinspired PEGylated hexaphenylalanine amyloid-like fibers and films [133]. Homogeneous peptide films with a very large area (40 mm 2 ), a low surface rugosity, and a thickness of 6-12 µm were simply prepared by drop-casting the peptide solutions on a glass substrate (Figure 12).…”

Section: Fluorescence In Pegylated Films and Fibersmentioning

confidence: 99%

“…Indeed, the full overlap of the FL spectrum and continuous optical absorbance generates a propagation of fluorescence over a long distance (hundreds of micrometers). This evidence was explained by introducing a novel model for the photon reabsorption for the anomalous long-range propagation of FL in PEG-F6 fibers, which is considered in the next chapter [133,134].…”

Section: Fluorescence In Pegylated Films and Fibersmentioning

confidence: 99%

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Fluorescence Phenomena in Amyloid and Amyloidogenic Bionanostructures

et al. 2020

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Nanoscale optical labeling is an advanced bioimaging tool. It is mostly based on fluorescence (FL) phenomena and enables the visualization of single biocells, bacteria, viruses, and biological tissues, providing monitoring of functional biosystems in vitro and in vivo, and the imaging-guided transportation of drug molecules. There is a variety of FL biolabels such as organic molecular dyes, genetically encoded fluorescent proteins (green fluorescent protein and homologs), semiconductor quantum dots, carbon dots, plasmonic metal gold-based nanostructures and more. In this review, a new generation of FL biolabels based on the recently found biophotonic effects of visible FL are described. This intrinsic FL phenomenon is observed in any peptide/protein materials folded into β-sheet secondary structures, irrespective of their composition, complexity, and origin. The FL effect has been observed both in natural amyloid fibrils, associated with neurodegenerative diseases (Alzheimer’s, Parkinson’s, and more), and diverse synthetic peptide/protein structures subjected to thermally induced biological refolding helix-like→β-sheet. This approach allowed us to develop a new generation of FL peptide/protein bionanodots radiating multicolor, tunable, visible FL, covering the entire visible spectrum in the range of 400–700 nm. Newly developed biocompatible nanoscale biomarkers are considered as a promising tool for emerging precise biomedicine and advanced medical nanotechnologies (high-resolution bioimaging, light diagnostics, therapy, optogenetics, and health monitoring).

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Section: Physical Mechanism Of Fl In Amyloid and Amyloidogenic Fibrilsmentioning

confidence: 96%

Section: Physical Mechanism Of Fl In Amyloid and Amyloidogenic Fibrilsmentioning

confidence: 96%

Section: Fluorescence In Pegylated Films and Fibersmentioning

confidence: 99%

Section: Fluorescence In Pegylated Films and Fibersmentioning

confidence: 99%

Section: Fluorescence In Pegylated Films and Fibersmentioning

confidence: 99%

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Fluorescence Phenomena in Amyloid and Amyloidogenic Bionanostructures

et al. 2020

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Fold‐Sensitive Visible Fluorescence in β‐Sheet Peptide Structures

Apter

Lapshina

Lapsker

et al. 2021

Advanced Optical Materials

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Fluorescence (FL) is a basic optical phenomenon that is widely used in molecular spectroscopy, high‐resolution microscopy, and bioimaging. It has exciting and emission spectra defined by an electronic structure of particular fluorochrome molecules. In this work, another type of FL is investigated. This FL phenomenon is observed both in biological amyloid and bioinspired amyloidogenic assemblies, folded into specific biomolecular β‐sheet secondary structure. A fine mechanism of this fold‐sensitive FL effect, arising at the earliest stages of seeding and nucleation of β‐sheet nanofibers, and its growth under thermally activated conformational refolding of helical to β‐sheet state in bioinspired ultrashort peptide structures are studied. This FL effect demonstrates different properties compared to the classical FL from non‐folded biomolecules or molecular dyes. Its excitation and emission spectra are similar in any β‐sheet peptide/protein assemblies irrespective of their biochemical composition, primary structure, and origin. It is shown that this fold‐sensitive FL effect exhibits a wideband spectrum, covering the entire visible region (400–650 nm), high quantum yield, and tunable FL wavelength. This research paves the way for advanced FL optical nanomaterials, for new methods of bioimaging and diagnostics as well as for the development of biocompatible nanoscale FL sources.

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Photon Recycling Effect and Lossless Fluorescence Propagation in β‐Sheet Peptide Fibers

Apter

Lapsker

Inberg

et al. 2021

Advanced Optical Materials

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nervous system, [4] cardia therapy (pacing and resynchronization), [5] bladder dysfunctions and pain treatment, [6] restoration of vision and hearing. [7] Optogenetics is based on microbial photosensitive proteins expressed in target neuron cells. These proteins act as ion pumps across the plasma membranes under light illumination, which covers the visible region 400-650 nm. It was found that activation (excitation) or silencing (inhibition) of neurons is performed by light illumination at different selective wavelengths. [2,7,8] To monitor single neuronal spiking and synaptic events, the photonic flux is modulated at a high frequency in a millisecond timescale to adopt the light illumination parameters to the kinetics of the neuronal system. [9] It allows optical deconstruction of Parkinson's disease circuitry by modulated visible light with frequency 20-130 Hz. [10] This lightinduced biomedical nanotechnology requires implanted light waveguiding probes coupled to either a continuous wideband optical source or narrow-band LED/monochromatic diode lasers. [11,12] The effect of the visible FL was discovered in two different large classes of biological fibrillary structures, associated with amyloid neurodegenerative diseases (Alzheimer, Parkinson, and more), [13][14][15][16] and bioinspired amyloidogenic synthetic ultrashort di-and tri-peptides fiber structures, [17][18][19] and as well as in a new generation of hybrid polymer/peptide thin films and fiber materials. [20][21][22] Regardless of a significant and fundamental difference in chemical composition, the origin and complexity of these biological (natural) and bioinspired (chemically synthesized) peptide/protein structures exhibit a few common features. They fold into the same β-sheet peptide secondary structure, share similar fibrillary morphology, and display completely similar FL spectra. [23] Visible fold-sensitive FL was ascribed to electron delocalization of hydrogen bonds in the β-sheet structure where molecular dynamics simulations demonstrated the proton transfer across the hydrogen bonds followed by lowering electron excitation energies and visible FL. [15,24] The role of the short hydrogen bonds in intrinsic visible FL of amyloid fibril structures was recently studied experimentally and theoretically. [25] This work reports on a new class of light-emitting and lightdelivering micro-and nanosources. We reveal a lossless FL radiation energy transport through β-sheet peptide fibers of 100 µm in length despite a high optical absorption coefficient. These Light-delivering optical fibers are widely used for biomedical imaging, theranostics and surgery, and optogenetics. In this work, a new generation of bioinspired optical fibers is proposed. Developed amyloidogenic peptide fibrillary structures with tailored β-sheet conformation exhibit unique optical properties of full overlapping of broadband visible fluorescence (FL) and optical absorption spectra. This study reports on unexpected lossless propagation of the FL light along 100 µm length β-sheet microf...

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Long‐Range Fluorescence Propagation in Amyloidogenic β‐Sheet Films and Fibers

Cited by 21 publications

References 58 publications

Fluorescence Phenomena in Amyloid and Amyloidogenic Bionanostructures

Fluorescence Phenomena in Amyloid and Amyloidogenic Bionanostructures

Fold‐Sensitive Visible Fluorescence in β‐Sheet Peptide Structures

Photon Recycling Effect and Lossless Fluorescence Propagation in β‐Sheet Peptide Fibers

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