Long Noncoding XLOC_006390 Regulates the Proliferation and Metastasis of Human Colorectal Cancer via miR-296/ONECUT2 Axis

Abstract

Long noncoding RNA (LncRNA) XLOC_006390 has been shown to be dysregulated in cancer tissues and regulates cancer growth and development. Nonetheless, the molecular role of lncRNA-XLOC_006390 in colorectal cancer via modulation of miR-296/ONECUT2 axis is still unclear. Against this backdrop, the current study was designed to explore the role of lncRNA-XLOC_006390 in colorectal cancer proliferation and metastasis. The results revealed significant ( P < 0.05 ) overexpression of lncRNA-XLOC_006390 in colorectal cancer tissues and cell lines, and the transcript levels increased with the advancement of the disease. Moreover, its high expression was shown to be associated with poor patient survival. Silencing of lncRNA-XLOC_006390 in colorectal cancer cells significantly ( P < 0.05 ) suppressed their viability via onset of apoptosis and restricted cancer cell migration and invasion. In vivo tumor growth was significantly ( P < 0.05 ) inhibited under lncRNA-XLOC_006390 repression. LncRNA-XLOC_006390 was shown to sponge the expression of miR-296-3p which in turn acted via post-transcriptional suppression of ONECUT 2 transcription factor to regulate the growth of colorectal cancer. Taken together, the results revealed the oncogenic role of lncRNA-XLOC_006390 in colorectal cancer via modulation of miR-296/ONECUT2 axis. The results also point towards its prognostic and therapeutic potential in the treatment of colorectal cancer.

The functions and mechanisms of long non-coding RNA in colorectal cancer

“…The silencing of SPINT1-AS1 mediates the expression of HDGF by targeting miR-214, increasing apoptosis in CRC cells ( 193 ). Ma et al.’s study reveals that silencing XLOC_006390 inhibits apoptosis in CRC through the regulation of the miR-296/ONECUT2 axis ( 207 ). Fang et al.…”

“…reported that downregulation of XIST inhibits migration and invasion in CRC cells by regulating the miR-338-3p/PAX5 axis ( 205 ). The silencing of XLOC006390 significantly inhibits their viability by inducing apoptosis and limiting the migration and invasion of cancer cells ( 207 ). Fang et al.’s study shows that ZFAS1 promotes CRC invasion, and its mechanism is associated with E-cadherin, ZO-1, vimentin, and N-cadherin ( 208 ).…”