Long Noncoding RNAs in Human Stemness and Differentiation

Azad, Fatemeh Mirzadeh; Polignano, Isabelle Laurence; Proserpio, Valentina; Oliviero, Salvatore

doi:10.1016/j.tcb.2021.02.002

Cited by 34 publications

(23 citation statements)

References 90 publications

(92 reference statements)

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“…Several canonical and non-canonical Bmp2 signaling pathways have been reported involved in odontogenesis ( Cho et al, 2010 ; Qin et al, 2012a ; Qin et al, 2012b ; Washio et al, 2012 ; Qin et al, 2014 ). Recently accumulated evidence has demonstrated that lncRNAs play biological roles in dental cell proliferation, cell proliferation, and tooth development through the regulation of growth and transcriptional factors ( Zheng and Jia, 2016 ; Liu et al, 2018 ; Gil and Ulitsky, 2020 ; Wang et al, 2020 ; Li et al, 2021 ; Mirzadeh Azad et al, 2021 ). The cross talk between the Bmp2 signaling pathway and lncRNAs in dental cell proliferation, cell differentiation, and odontogenesis remains largely unclear.…”

Section: Discussionmentioning

confidence: 99%

Differential lncRNA/mRNA Expression Profiling and Functional Network Analyses in Bmp2 Deletion of Mouse Dental Papilla Cells

et al. 2021

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Bmp2 is essential for dentin development and formation. Bmp2 conditional knock-out (KO) mice display a similar tooth phenotype of dentinogenesis imperfecta (DGI). To elucidate a foundation for subsequent functional studies of cross talk between mRNAs and lncRNAs in Bmp2-mediated dentinogenesis, we investigated the profiling of lncRNAs and mRNAs using immortalized mouse dental Bmp2 flox/flox (iBmp2fx/fx) and Bmp2 knock-out (iBmp2ko/ko) papilla cells. RNA sequencing was implemented to study the expression of the lncRNAs and mRNAs. Quantitative real-time PCR (RT-qPCR) was used to validate expressions of lncRNAs and mRNAs. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases were used to predict functions of differentially expressed genes (DEGs). Protein–protein interaction (PPI) and lncRNA–mRNA co-expression network were analyzed by using bioinformatics methods. As a result, a total of 22 differentially expressed lncRNAs (16 downregulated vs 6 upregulated) and 227 differentially expressed mRNAs (133 downregulated vs. 94 upregulated) were identified in the iBmp2ko/ko cells compared with those of the iBmp2fx/fx cells. RT-qPCR results showed significantly differential expressions of several lncRNAs and mRNAs which were consistent with the RNA-seq data. GO and KEGG analyses showed differentially expressed genes were closely related to cell differentiation, transcriptional regulation, and developmentally relevant signaling pathways. Moreover, network-based bioinformatics analysis depicted the co-expression network between lncRNAs and mRNAs regulated by Bmp2 in mouse dental papilla cells and symmetrically analyzed the effect of Bmp2 during dentinogenesis via coding and non-coding RNA signaling.

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Section: Discussionmentioning

confidence: 99%

Differential lncRNA/mRNA Expression Profiling and Functional Network Analyses in Bmp2 Deletion of Mouse Dental Papilla Cells

et al. 2021

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“…Considering the human brain, this is by far the most complex and diversified organ in terms of the cellular types, neuronal and glial. This complexity is reflected in its great number of unique cell-type-specific lncRNAs and was linked to the evolution of the human brain itself, in terms of its morphology and functions [ 163 , 164 , 165 ].…”

Section: The Search For Relevant Lncrnas Using Integrated Approachesmentioning

confidence: 99%

The Emerging Roles of Long Non-Coding RNAs in Intellectual Disability and Related Neurodevelopmental Disorders

Liaci

Prandi

Pavinato

et al. 2022

IJMS

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In the human brain, long non-coding RNAs (lncRNAs) are widely expressed in an exquisitely temporally and spatially regulated manner, thus suggesting their contribution to normal brain development and their probable involvement in the molecular pathology of neurodevelopmental disorders (NDD). Bypassing the classic protein-centric conception of disease mechanisms, some studies have been conducted to identify and characterize the putative roles of non-coding sequences in the genetic pathogenesis and diagnosis of complex diseases. However, their involvement in NDD, and more specifically in intellectual disability (ID), is still poorly documented and only a few genomic alterations affecting the lncRNAs function and/or expression have been causally linked to the disease endophenotype. Considering that a significant fraction of patients still lacks a genetic or molecular explanation, we expect that a deeper investigation of the non-coding genome will unravel novel pathogenic mechanisms, opening new translational opportunities. Here, we present evidence of the possible involvement of many lncRNAs in the etiology of different forms of ID and NDD, grouping the candidate disease-genes in the most frequently affected cellular processes in which ID-risk genes were previously collected. We also illustrate new approaches for the identification and prioritization of NDD-risk lncRNAs, together with the current strategies to exploit them in diagnosis.

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“…Stem cells are widely involved in body growth and development and organ formation, and have the ability of self-renewal, infinite proliferation and multidirectional differentiation ( Jing et al, 2021 ; Mehraj et al, 2021 ; Mirzadeh Azad et al, 2021 ; Otero-Albiol and Carnero, 2021 ). The strong self-renewal ability, inherent high proliferative capacity and multidirectional differentiation together constitute the basic characteristics of malignant stem cells, among which the self-renewal ability is closely related to tumorigenesis and malignancy ( Ferragut et al, 2021 ; Mehraj et al, 2021 ; Yue et al, 2021 ).…”

Section: Biological Function and Molecular Mechanism Of Circular Rnas In Glioblastomamentioning

confidence: 99%

Research Progress of circRNAs in Glioblastoma

Guo

Piao

2021

Front. Cell Dev. Biol.

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Circular RNAs (circRNAs) are a class of single-stranded covalently closed non-coding RNAs without a 5′ cap structure or 3′ terminal poly (A) tail, which are expressed in a variety of tissues and cells with conserved, stable and specific characteristics. Glioblastoma (GBM) is the most aggressive and lethal tumor in the central nervous system, characterized by high recurrence and mortality rates. The specific expression of circRNAs in GBM has demonstrated their potential to become new biomarkers for the development of GBM. The specific expression of circRNAs in GBM has shown their potential as new biomarkers for GBM cell proliferation, apoptosis, migration and invasion, which provides new ideas for GBM treatment. In this paper, we will review the biological properties and functions of circRNAs and their biological roles and clinical applications in GBM.

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Long Noncoding RNAs in Human Stemness and Differentiation

Cited by 34 publications

References 90 publications

Differential lncRNA/mRNA Expression Profiling and Functional Network Analyses in Bmp2 Deletion of Mouse Dental Papilla Cells

Differential lncRNA/mRNA Expression Profiling and Functional Network Analyses in Bmp2 Deletion of Mouse Dental Papilla Cells

The Emerging Roles of Long Non-Coding RNAs in Intellectual Disability and Related Neurodevelopmental Disorders

Research Progress of circRNAs in Glioblastoma

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