Long Non-Coding RNA Small Nucleolar RNA Host Gene 5 (SNHG5) Regulates Renal Tubular Damage in Diabetic Nephropathy via Targeting MiR-26a-5p

Abstract

The study explored the diagnostic value of SNHG5 in diabetic nephropathy (DN) and investigated the role and mechanism on DN via establishing the in vitro HK2 cell model. This study recruited 62 types 2 diabetes mellitus (T2DM) patients, 58 DN patients and 60 healthy controls (HC). The expressions of serum SNHG5 and miR-26a-5p were measured by RT-qPCR analysis. The diagnostic value of SNHG5 in DN was assessed by ROC curve. The in vitro cell model was built to estimate the effects of SNHG5 on cell viability, cell apoptosis, inflammation response and oxidative stress. Serum SNHG5 was increased in DN patients (relative expression: 2.04±0.34) and had the diagnostic value in DN. After HK2 cells were treated with high glucose, the cell viability decreased and apoptosis increased, and the production of inflammatory cytokines and ROS enhanced significantly. It was noticed that inhibition of SNHG5 could reverse the above phenomenon caused by high glucose. Besides, serum miR-26a-5p was diminished in DN patients, and luciferase reporter gene revealed that miR-26a-5p is direct target of SNHG5. These results indicated that inhibition of SNHG5 may mitigate HG-induced renal tubular damage via targeting miR-26a-5p, which providing a new insight into the mechanism of renal tubule damage in DN patients.

Long noncoding RNAs as potential diagnostic biomarkers for diabetes mellitus and complications: A systematic review and meta‐analysis

“…In addition to the 103 review articles, a total of 246 studies were removed for the following reasons: 120 were animal studies, 108 did not report AUC values, and 18 did not report numerical sensitivity or specificity results. In total, 24 studies ultimately met our eligibility criteria, of which T2DM, DN, and DR were analyzed in 17, 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 5, 5 , 30 , 35 , 36 , 37 and 7 studies, 4 , 28 , 29 , 32 , 37 , 38 , 39 respectively (some articles included more than one of these outcomes). Table 1 provides the specifics of the survey characteristics and summarizes the quality assessment results.…”

The miR-26a-5p/IL-6 axis alleviates sepsis-induced acute kidney injury by inhibiting renal inflammation

“…Further, Cat et al. demonstrated that inhibition of Long Non-Coding RNA Small Nucleolar RNA Host Gene 5 (SNHG5) might mitigate HG-induced renal tubular damage via targeting miR-26a-5p [ 38 ].…”

Long noncoding RNA SNHG5 promotes podocyte injury via the microRNA-26a-5p/TRPC6 pathway in diabetic nephropathy

“…Recently, compelling evidence indicated that upregulated SNHG5 plays a vital role in neoplastic kidney disease ( 34 , 35 ). Regarding nonneoplastic kidney disease, a recent study indicated that SNHG5 expression was elevated in HK-2 cells treated with HG, accompanied with decreased cell viability, increased apoptosis, and enhanced inflammatory cytokines and oxidative stress ( 36 ). Nevertheless, the potential effects of SNHG5 in podocytes remain to be explored.…”