Long non‑coding RNA RP11‑84C13.1 promotes osteogenic differentiation of bone mesenchymal stem cells and alleviates osteoporosis progression via the miR‑23b‑3p/RUNX2 axis

Abstract

The objective of the present study was to determine the role of RP11-84C13.1 in osteoporosis (OP) and its molecular mechanism. First, clinical samples were collected from OP patients and normal control patients. Human bone marrow stromal cells (hBMSCs) were extracted from femoral head tissues. Runt-related transcription factor 2 (RUNX2) and RP11-84C13.1 serum levels were assessed by reverse transcription-quantitative (RT-q)PCR. Following transfection of pcDNA-RP11-84C13.1, si-RP11-84C13.1, microRNA (miRNA)-23b-3p mimic and miRNA-23b-3p inhibitor, the expression levels of RUNX2 and RP11-84C13.1 were determined by RT-qPCR. In addition, the osteogenic ability of hBMSCs was assessed by Alizarin Red staining. The binding of RP11-84C13.1 to miRNA-23b-3p and the binding of miRNA-23b-3p to RUNX2 was confirmed by dual-luciferase reporter gene assay. Long non-coding RNA (lncRNA) RP11-84C13.1 was significantly downregulated in the serum of OP patients. The osteogenic differentiation-related genes RUNX2 and RP11-84C13.1 were markedly upregulated in a time-dependent manner, while the miRNA-23b-3p level gradually decreased in hBMSCs with the prolongation of osteogenesis. RP11-84C13.1 knockdown inhibited the osteogenic differentiation of hBMSCs. Furthermore, RP11-84C13.1 regulated RUNX2 expression by targeting miRNA-23b-3p. Overexpression of miRNA-23b-3p partially reversed the promoting effect of RP11-84C13.1 on the osteogenesis of hBMSCs. In conclusion, lncRNA RP11-84C13.1 upregulated RUNX2 by absorbing miRNA-23b-3p, and thus induced hBMSC osteogenesis to alleviate osteoporosis.

Myoblast-derived exosomal Prrx2 attenuates osteoporosis via transcriptional regulation of lncRNA-MIR22HG to activate Hippo pathway

“…Mounting evidence has indicated that lncRNAs absorbed miRNAs to affect the expression of osteogenic differentiation-related genes in osteoporosis progression. Yu et al, documented that lncRNA RP11-84C13.1 led to improvement in capability of osteogenic differentiation by enhancing RUNX2 expression via sequestering miR-23b-3p (Yu et al 2021 ). A recent study showed that miR-128 was highly expressed in osteoporotic patients, enforced expression of miR-128 suppressed osteoblast differentiation via targeting SIRT6 (Zhao et al 2019 ).…”

lncRNA FGD5-AS1 Regulates Bone Marrow Stem Cell Proliferation and Apoptosis by Affecting miR-296-5p/STAT3 Axis in Steroid-Induced Osteonecrosis of the Femoral Head

“…Previous studies have shown that the osteogenic differentiation of BMSCs is associated with the progression of orthopedic diseases. For example, lncRNA RP11-84C13.1 promoted the osteogenic differentiation of BMSCs and alleviated osteoporosis progression via the miR-23b-3p/RUNX2 axis [ 19 ]. Huang et al reported that Circ_0067680 expedited the osteogenic differentiation of hBMSCs through the miR-4429/CTNNB1/Wnt/ β -catenin pathway [ 20 ].…”

The management of bone defect using long non-coding RNA as a potential biomarker for regulating the osteogenic differentiation process

“…Similarly, lncRNA TCONS_00041960 regulated Runx2 and GILZ by interacting with miR-204-5p and miR-125a-3p, respectively, enhancing the osteogenic effect and inhibiting its lipogenic effect of rBMSCs [ 55 ]. Furthermore, lncRNAs HHAS1 [ 56 ], RP11-84C13.1 [ 57 ], and LINC00899 [ 58 ] enhanced Runx2 level by downregulating miR-204-5p, miR-23b-3p, and miR-374a expression respectively to facilitate osteogenic differentiation. The aforementioned up-regulation of multiple lncRNAs can promote osteogenesis through the ceRNA mechanism, which illustrates its universality and importance.…”