Long‐Lived Cells and Long‐Lived Proteins in the Human Body

Truscott, Roger J.W.

doi:10.1002/9783527826759.ch1

Cited by 1 publication

(2 citation statements)

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“…Throughout the body, long-lived cells and long-lived proteins (LLPs) are more common than previously thought. − From brain to testis, many cells have been discovered to have a long lifespan . Within these cells, a number of LLPs have been found .…”

Section: Introductionmentioning

confidence: 99%

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Spontaneous Cleavage at Glu and Gln Residues in Long-Lived Proteins

et al. 2021

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Long-lived proteins (LLPs) are prone to deterioration with time, and one prominent breakdown process is the scission of peptide bonds. These cleavages can either be enzymatic or spontaneous. In this study, human lens proteins were examined and many were found to have been cleaved on the C-terminal side of Glu and Gln residues. Such cleavages could be reproduced experimentally by in vitro incubation of Glu- or Gln-containing peptides at physiological pHs. Spontaneous cleavage was dependent on pH and amino acid sequence. These model peptide studies suggested that the mechanism involves a cyclic intermediate and is therefore analogous to that characterized for cleavage of peptide bonds adjacent to Asp and Asn residues. An increased amount of some Glu/Gln cleaved peptides in the insoluble fraction of human lenses suggests that cleavage may act to destabilize proteins. Spontaneous cleavage at Glu and Gln, as well as recently described cross-linking at these residues, can therefore be added to the similar processes affecting long-lived proteins that have already been documented for Asn and Asp residues.

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Section: Introductionmentioning

confidence: 99%

“…1−3 From brain to testis, many cells have been discovered to have a long lifespan. 4 Within these cells, a number of LLPs have been found. 5 The lifetimes of these proteins lead to an array of modifications; both enzymatic and spontaneous.…”

Section: ■ Introductionmentioning

confidence: 99%