Locked Nucleic Acids as MicroRNA Therapeutics

Ørum, Henrik

doi:10.1002/9781118300312.ch40

Cited by 3 publications

(4 citation statements)

References 40 publications

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“…37 One of the Anti-miRs is LNA that was used to inhibit oncomiRs. 23 In this study, it was determined that degradation of miR-21 with LNA-anti-miR could prevent proliferation of HepG2 cells and seemed that miR-21 is an oncomiR in HCC. In treatment of cancer, the destruction of cells with uncontrolled proliferation seems to be an aim.…”

Section: Discussionmentioning

confidence: 99%

“…4,20-22 Locked nucleic acid (LNA) is a new lineage of modified oligonucleotide that has a 2'-O 4'-C Methylene bridge in its structure leading to consistently high temperature and resistance to enzymatic degradation. [23][24][25] The effect and safety of LNA-anti-miRs for degradation of miRNAs have been shown in several studies, suggesting them as suitable candidates for therapeutic applications. 23,26 In this study, we have used LNA-anti-miR for degradation of miR-21 in HCC.…”

Section: Introductionmentioning

confidence: 99%

“…[23][24][25] The effect and safety of LNA-anti-miRs for degradation of miRNAs have been shown in several studies, suggesting them as suitable candidates for therapeutic applications. 23,26 In this study, we have used LNA-anti-miR for degradation of miR-21 in HCC. Cell proliferation, apoptosis and necrosis were assessed.…”

Section: Introductionmentioning

confidence: 99%

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Degradation of miR-21 induces apoptosis and inhibits cell proliferation in human hepatocellular carcinoma

2015

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MicroRNAs (miRNAs) are small non-coding RNAs, 18-25 nucleotides long and have an important role in post-transcriptional regulation of gene. Several aspects of cellular activities such as cell growth, proliferation and differentiation are regulated by miRNAs. In many cancers and malignancies, up- or downregulation of different miRNAs has been reported. In human hepatocellular carcinoma (HCC), upregulation of miR-21 has been reported in human in vitro studies. Here, we made an assessment of the effect of miR-21 degradation on viability and apoptosis of HCC cell line (HepG2) using locked nucleic acid (LNA). At different time points (24, 48, 72 h) after LNA-anti-miR-21 transfection, 3-[4,5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium bromide assay and Annexin/propidium iodide staining were performed. The results show that miR-21 degradation can decrease the viability of cells, mainly by induction of apoptosis and necrosis. These findings suggest that degradation of miR-21 could be used as a novel approach in treatment of HCC.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Degradation of miR-21 induces apoptosis and inhibits cell proliferation in human hepatocellular carcinoma

2015

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“…In an miRNA-based therapeutic strategy, the miRNA expression, which is altered in different diseases, is corrected by injecting the downregulated miRNAs or by inhibiting the upregulated ones. Antisense miRNAs that have been chemically transformed to have improved durability are used in locked nucleic acid (LNA) or antagomirs administered in nanoparticle form as present therapeutics to downregulate miRNA expression [ 132 , 133 ]. miRNA mimics are similarly used to restore miRNA function.…”

Section: Therapeutic Considerationsmentioning

confidence: 99%

miRNAs in Herpesvirus Infection: Powerful Regulators in Small Packages

Dass

Dhotre

Chakraborty

et al. 2023

Viruses

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microRNAs are a class of small, single-stranded, noncoding RNAs that regulate gene expression. They can be significantly dysregulated upon exposure to any infection, serving as important biomarkers and therapeutic targets. Numerous human DNA viruses, along with several herpesviruses, have been found to encode and express functional viral microRNAs known as vmiRNAs, which can play a vital role in host–pathogen interactions by controlling the viral life cycle and altering host biological pathways. Viruses have also adopted a variety of strategies to prevent being targeted by cellular miRNAs. Cellular miRNAs can act as anti- or proviral components, and their dysregulation occurs during a wide range of infections, including herpesvirus infection. This demonstrates the significance of miRNAs in host herpesvirus infection. The current state of knowledge regarding microRNAs and their role in the different stages of herpes virus infection are discussed in this review. It also delineates the therapeutic and biomarker potential of these microRNAs in future research directions.

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