Localization of vicilin genes via polymerase chain reaction on microisolated field bean chromosomes

Abstract

SummaryA new technique is reported for the physical mapping of low copy DNA sequences on plant chromosomes. Individual chromosomes were microisolated and their DNA used as the target for the polymerase chain reaction in order to identify the chromosome carrying a specific gene sequence. The use of defined translocation chromosomes further refined the resolution of the method to a subchromosomal level. To demonstrate the applicability of the procedure genes have been localized coding for vicilin seed storage proteins on the field bean Vicia faba L. in a region which includes the centromere and the proximal parts of the short and the long arms of chromosome II.

Construction of a high-density genetic map for faba bean (Vicia faba L.) and quantitative trait loci mapping of seed-related traits

“…At present, there are relatively few studies on the QTL mapping of quality traits in broad beans. Only five genes that control grain proteins have been identified ( Macas et al 1993b ). In this study, 22 QTLs linked to quality traits were detected using SNP markers for the first time, including 7 QTLs for FC, 7 for StC, 4 for LC, 2 for PC, and 2 for TC ( Supplementary Table S6 ).…”

Seed Storage Proteins of Faba Bean (Vicia faba L): Current Status and Prospects for Genetic Improvement

“…67 The vicilin coding gene 70 was also located on chromosome II, near the centromere. 71,72 While the documented number of genes for Vf globulins is relatively small, numerous legumin and vicilin minor subunits with various molecular masses and isoelectric points can be observed in 2D gel electrophoresis analysis, 51 suggesting that Vf globulins undergo extensive post-translational processing. A similar occurrence has been found in other legumes, including Medicago truncatula 73 and Pisum sativum.…”