Localization of the Human Cytoplasmic Dynein Heavy Chain (DNECL) to 14qter by Fluorescence in Situ Hybridization

We report the molecular and genetic characterization of the cytoplasmic dynein light-chain gene, ddlc1, from Drosophila melanogaster. ddlc1 encodes the first cytoplasmic dynein light chain identified, and its genetic analysis represents the first in vivo characterization of cytoplasmic dynein function in higher eucaryotes. The ddlc1 gene maps to 4E1-2 and encodes an 89-amino-acid polypeptide with a high similarity to the axonemal 8-kDa outer-arm dynein light chain from Chlamydomonas flagella. Developmental Northern (RNA) blot analysis and ovary and embryo RNA in situ hybridizations indicate that the ddlc1 gene is expressed ubiquitously. Anti-DDLC1 antibody analyses show that the DDLC1 protein is localized in the cytoplasm. P-element-induced partial-loss-of-function mutations cause pleiotropic morphogenetic defects in bristle and wing development, as well as in oogenesis, and hence result in female sterility. The morphological abnormalities found in the ovaries are always associated with a loss of cellular shape and structure, as visualized by a disorganization of the actin cytoskeleton. Total-loss-of-function mutations cause lethality. A large proportion of mutant animals degenerate during embryogenesis, and the dying cells show morphological changes characteristic of apoptosis, namely, cell and nuclear condensation and fragmentation, as well as DNA degradation. Cloning of the human homolog of the ddlc1 gene, hdlc1, demonstrates that the dynein light-chain 1 is highly conserved in flies and humans. Northern blot analysis and epitope tagging show that the hdlc1 gene is ubiquitously expressed and that the human dynein light chain 1 is localized in the cytoplasm. hdlc1 maps to 14q24.

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“…hdlc1 is localized to chromosome 14q24. The human cytoplasmic dynein heavy chain was mapped to 14q (24).…”

Section: Ins3mentioning

confidence: 99%

Cytoplasmic Dynein (ddlc1) Mutations Cause Morphogenetic Defects and Apoptotic Cell Death in Drosophila melanogaster

Dick

Ray

Salz

et al. 1996

Molecular and Cellular Biology

162

163

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“…These probes were mapped to a region syntenic to 14qter in humans [Vaughan et al, 1996]. The putative human cytoplasmic dynein heavy chain also has been mapped to the chromosome 14qter by fluorescence in situ hybridisation (FISH) [Narayan et al, 1994] as well as a human P1 clone bearing the cytoplasmic dynein light chain ddlc1 [Dick et al, 1996a]. Vaughan et al [1996] mapped the second cytoplasmic dynein heavy chain to region syntenic to 7q in humans.…”

Section: Chromosome Map Positions Of Dynein and Dynein-related Genesmentioning

confidence: 99%

Dynein and dynein-related genes

Milisav

1998

Cell Motil. Cytoskeleton

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“…(3) the dynein heavy-chain gene located on 14qter (Narayan et al 1994a); and (4) numerous other dynein, nexin, and other microtubule-related genes rapidly accumulating in the genomics databases. Many genes have been implicated recently in the control of the left-right asymmetry of body development such as that observed in KS (Overbeek 1997;Srivastava 1997;Wood 1997;Levin and Mercola 1998).…”

Section: Figurementioning

confidence: 99%

Two Novel Single–Base-Pair Substitutions Adjacent to the CAG Repeat in the Huntington Disease Gene (IT15): Implications for Diagnostic Testing

Margolis

Stine

Callahan

et al. 1999

The American Journal of Human Genetics

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Cancer results from the expansion of cell clones that progressively lose control of proliferation, differentiation, and death, owing to accumulation of mutational events in genes that control the cell cycle and apoptosis. Nuclear protein p53 is thought to play a major role in malignancy, since it induces genes that determine apoptosis and cell-cycle arrest, interacts with proteins employed in DNA repair, and binds to DNA strand breaks. As expected, somatic mutations in p53 are found in a variety of human cancers. Mutations are predominantly inactivating, thus eliminating the "guardian of the genome" from the proliferating cells. Germ-line mutations

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Localization of the Human Cytoplasmic Dynein Heavy Chain (DNECL) to 14qter by Fluorescence in Situ Hybridization

Cited by 11 publications

References 0 publications

Cytoplasmic Dynein (ddlc1) Mutations Cause Morphogenetic Defects and Apoptotic Cell Death in Drosophila melanogaster

Cytoplasmic Dynein (ddlc1) Mutations Cause Morphogenetic Defects and Apoptotic Cell Death in Drosophila melanogaster

Dynein and dynein-related genes

Two Novel Single–Base-Pair Substitutions Adjacent to the CAG Repeat in the Huntington Disease Gene (IT15): Implications for Diagnostic Testing

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