Localization of nucleolin binding sites on human and mouse pre-ribosomal RNA

Association of Nonribosomal Nucleolar Proteins in Ribonucleoprotein Complexes during Interphase and Mitosis

“…Efforts are currently underway to examine in detail the identity of this species, as well as of other intermediates that may be present in the complexes. The association of mature 18S and 28S rRNA with nucleolin in the immunopurified complexes is also consistent with previous experiments that mapped binding sites for nucleolin on 18S and 28S sequences (Herrera and Olson, 1986;Bugler et al, 1987;Ghisolfi-Nieto et al, 1996;Serin et al, 1996). Therefore, taken together, these results support a continued association of nucleolin with 18S and 28S rRNA sequences all the way from their emergence from the transcription machinery, as part of the primary transcript, to their final processing into mature 18S and 28S rRNA molecules.…”

Association of Nonribosomal Nucleolar Proteins in Ribonucleoprotein Complexes during Interphase and Mitosis

“…Efforts are currently underway to examine in detail the identity of this species, as well as of other intermediates that may be present in the complexes. The association of mature 18S and 28S rRNA with nucleolin in the immunopurified complexes is also consistent with previous experiments that mapped binding sites for nucleolin on 18S and 28S sequences (Herrera and Olson, 1986;Bugler et al, 1987;Ghisolfi-Nieto et al, 1996;Serin et al, 1996). Therefore, taken together, these results support a continued association of nucleolin with 18S and 28S rRNA sequences all the way from their emergence from the transcription machinery, as part of the primary transcript, to their final processing into mature 18S and 28S rRNA molecules.…”

Isolation and proteomic characterization of the major proteins of the nucleolin-binding ribonucleoprotein complexes

“…This is supported by our results with the domain-deletion mutants and by UV-light induced cross-linking assay on nuclear extract [8]. In addition, NCL has been reported to be present in association with nascent pre-rRNA in about the same quantity in fractionated nucleolus.…”

miR‐34b Modulates Skeletal Muscle Cell Proliferation and Differentiation

“…On the other hand, functions of NUCL are associated with its subcellular location. NUCL was localized to the nucleolus when it participated in ribosomal RNA transcription and maturation [Serin et al, 1996;Ginisty et al, 1998], and within the nucleoplasm when regulating transcription of genes by binding to their promoters, such as the VEGF promoter [Uribe et al, 2011]. In this study, simultaneously, the amount of NUCL in the nucleus was decreased and that in the cytoplasm was increased at DM1 (Fig.…”

Association of Nonribosomal Nucleolar Proteins in Ribonucleoprotein Complexes during Interphase and Mitosis

“…Efforts are currently underway to examine in detail the identity of this species, as well as of other intermediates that may be present in the complexes. The association of mature 18S and 28S rRNA with nucleolin in the immunopurified complexes is also consistent with previous experiments that mapped binding sites for nucleolin on 18S and 28S sequences (Herrera and Olson, 1986;Bugler et al, 1987;Ghisolfi-Nieto et al, 1996;Serin et al, 1996). Therefore, taken together, these results support a continued association of nucleolin with 18S and 28S rRNA sequences all the way from their emergence from the transcription machinery, as part of the primary transcript, to their final processing into mature 18S and 28S rRNA molecules.…”

Isolation and proteomic characterization of the major proteins of the nucleolin-binding ribonucleoprotein complexes

“…This is supported by our results with the domain-deletion mutants and by UV-light induced cross-linking assay on nuclear extract [8]. In addition, NCL has been reported to be present in association with nascent pre-rRNA in about the same quantity in fractionated nucleolus.…”

miR‐34b Modulates Skeletal Muscle Cell Proliferation and Differentiation

“…On the other hand, functions of NUCL are associated with its subcellular location. NUCL was localized to the nucleolus when it participated in ribosomal RNA transcription and maturation [Serin et al, 1996;Ginisty et al, 1998], and within the nucleoplasm when regulating transcription of genes by binding to their promoters, such as the VEGF promoter [Uribe et al, 2011]. In this study, simultaneously, the amount of NUCL in the nucleus was decreased and that in the cytoplasm was increased at DM1 (Fig.…”

Association of Nonribosomal Nucleolar Proteins in Ribonucleoprotein Complexes during Interphase and Mitosis

“…Efforts are currently underway to examine in detail the identity of this species, as well as of other intermediates that may be present in the complexes. The association of mature 18S and 28S rRNA with nucleolin in the immunopurified complexes is also consistent with previous experiments that mapped binding sites for nucleolin on 18S and 28S sequences (Herrera and Olson, 1986;Bugler et al, 1987;Ghisolfi-Nieto et al, 1996;Serin et al, 1996). Therefore, taken together, these results support a continued association of nucleolin with 18S and 28S rRNA sequences all the way from their emergence from the transcription machinery, as part of the primary transcript, to their final processing into mature 18S and 28S rRNA molecules.…”

Isolation and proteomic characterization of the major proteins of the nucleolin-binding ribonucleoprotein complexes

“…This is supported by our results with the domain-deletion mutants and by UV-light induced cross-linking assay on nuclear extract [8]. In addition, NCL has been reported to be present in association with nascent pre-rRNA in about the same quantity in fractionated nucleolus.…”

miR‐34b Modulates Skeletal Muscle Cell Proliferation and Differentiation

“…On the other hand, functions of NUCL are associated with its subcellular location. NUCL was localized to the nucleolus when it participated in ribosomal RNA transcription and maturation [Serin et al, 1996;Ginisty et al, 1998], and within the nucleoplasm when regulating transcription of genes by binding to their promoters, such as the VEGF promoter [Uribe et al, 2011]. In this study, simultaneously, the amount of NUCL in the nucleus was decreased and that in the cytoplasm was increased at DM1 (Fig.…”