Localization of Cathepsin K and Tartrate‐Resistant Acid Phosphatase in Synovium and Cranial Cruciate Ligament in Dogs with Cruciate Disease

Muir, Peter; Schamberger, Gwenn M.; Manley, Paul A.; Hao, Zhengling

doi:10.1111/j.1532-950x.2005.00036.x

Cited by 52 publications

(75 citation statements)

References 39 publications

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“…Furthermore, a higher number of TRAP positive cells was seen in the synovium of the experimental group but few cells or none were seen in the control group. Histochemistry for TRAP produced results was consistent with those of an earlier study which reported an increase in the number of TRAP + cells in the synovium from humans with arthritis and dogs with osteoarthritis secondary to the naturally occurring rupture of the cranial cruciate ligament (Konttinen et al, 2001;Muir et al, 2005). These results suggest that an increase in the number of TRAP + cells in the synovium is related to the development of OA.…”

Section: Discussionsupporting

confidence: 82%

“…Recent reports have shown that the synovial intima of OA contains a large number of TRAP-containing cells (TRAP + cells) such as dendritic cells and macrophage-like cells (Muir et al, 2005). It was demonstrated that TRAP positive cells play an important role in cytokine synthesis, in the expression of MMPs and cartilage damage (Tsuboi et al, 2003;Adamopoulos et al, 2006b).…”

mentioning

confidence: 99%

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Tartrate-resistant acid phosphatase, matrix metalloproteinase-2 and tissue inhibitor metalloproteinase-2 in early stages of canine osteoarthritis

Lee¹,

Alam²,

Seol³

et al. 2008

Vet. Med. - Czech

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ABSTRACT:The aim of this study was to determine if the activity of tartrate-resistant acid phosphatase (TRAP) in the synovial fluid (SF) and serum can be used as a marker for diagnosing the early stages of osteoarthritis (OA). We also wished to determine if identifiable differences in the concentrations of matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of matrix metalloproteinase-2 (TIMP-2) could be detected in SF between normal joints and OA joints for the diagnosis of early OA. Ten skeletally mature beagle dogs underwent a unilateral surgical transection of the cranial cruciate ligament and medial meniscectomy. Five sham-operated beagle dogs were used as controls. The synovial fluid was collected in 1, 2 and 3 months and examined by western blotting for MMP-2 and ELISA for TIMP-2. The activity of TRAP in the SF and serum was measured using a spectrophotometer. In addition, the presence of TRAP positive cells in the synovium was identified by enzyme histochemistry. The level of the activity of TRAP and MMP-2 in the SF from the induced OA dogs was significantly higher than that of the control over a three-month period (P < 0.05). The TIMP-2 level in the SF was significantly lower in the induced OA dogs than in the control. However, there was no difference in TRAP activity in the serum. Histochemistry revealed a higher number of TRAP positive cells in the synovium from the induced OA dogs. Based on these data, we conclude that the activity of TRAP, MMP-2 and TIMP-2 in SF can be used as a biomarker to diagnose and monitor the early stages of OA.Keywords: tartrate-resistant acid phosphatase; matrix metalloproteinase-2; tissue inhibitor of matrix metalloproteinase-2; osteoarthritis; synovial fluid Supported by the Second Stage Brain Korea (BK) 21 Project and the Korea Science and Engineering Foundation

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Section: Discussionsupporting

confidence: 82%

mentioning

confidence: 99%

Tartrate-resistant acid phosphatase, matrix metalloproteinase-2 and tissue inhibitor metalloproteinase-2 in early stages of canine osteoarthritis

Lee¹,

Alam²,

Seol³

et al. 2008

Vet. Med. - Czech

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“…TRAP + mononuclear cells are activated macrophages typically found in synovium of dogs with CR, but not in normal synovium [11]. In the present study, we found that a majority of synovial biopsies from both the index stifle (83%) and the contralateral stifle (67%) contained TRAP + macrophages.…”

Section: Discussionsupporting

confidence: 47%

“…The arthroscopic synovial assessment method we used was adapted from a validated scoring system used in human beings [27]. Our selection of histologic, serum, and synovial fluid markers was chosen on the basis of past work that has shown that inflamed synovium in CR stifles typically contains a mixed population of mononuclear cells, often aggregated into nodules [10],[11]. We also determined intra- and inter-observer variability to help validate our arthroscopic assessment method.…”

Section: Discussionmentioning

confidence: 99%

“…The inflammatory cell population is principally mononuclear and includes T and B lymphocytes, activated tartrate-resistant acid phosphatase-positive (TRAP + ) macrophages, major histocompatibility complex (MHC) class II + dendritic cells, and plasma cells [9]–[11]. Fraying and degeneration of the caudal cruciate ligament is also common in affected dogs [12].…”

Section: Introductionmentioning

confidence: 99%

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Arthroscopic Assessment of Stifle Synovitis in Dogs with Cranial Cruciate Ligament Rupture

et al. 2014

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Cranial cruciate ligament rupture (CR) is a degenerative condition in dogs that typically has a non-contact mechanism. Subsequent contralateral rupture often develops in dogs with unilateral CR. Synovitis severity is an important factor that promotes ligament degradation. Consequently, we wished to evaluate the utility of arthroscopy for assessment of stifle synovitis in dogs with CR. Herein, we report results of a prospective study of 27 dogs with unilateral CR and bilateral radiographic osteoarthritis. Arthroscopic images and synovial biopsies from the lateral and medial joint pouches were obtained bilaterally and graded for synovial hypertrophy, vascularity, and synovitis. Synovial tartrate-resistant acid phosphatase-positive (TRAP+) macrophages, CD3+ T lymphocytes, Factor VIII+ blood vessels, and synovial intima thickness were quantified histologically and related to arthroscopic observations. Risk of subsequent contralateral CR was examined using survival analysis. We found that arthroscopic scores were increased in the index stifle, compared with the contralateral stifle (p<0.05). Numbers of CD3+ T lymphocytes (SR = 0.50, p<0.05) and TRAP+ cells in joint pouches (SR = 0.59, p<0.01) were correlated between joint pairs. Arthroscopic grading of vascularity and synovitis was correlated with number density of Factor VIII+ vessels (SR>0.34, p<0.05). Arthroscopic grading of villus hypertrophy correlated with numbers of CD3+ T lymphocytes (SR = 0.34, p<0.05). Synovial intima thickness was correlated with arthroscopic hypertrophy, vascularity, and synovitis (SR>0.31, p<0.05). Strong intra-observer and moderate inter-observer agreement for arthroscopic scoring was found. Dog age and arthroscopic vascularity significantly influenced risk of contralateral CR over time. We conclude that arthroscopic grading of synovitis is a precise tool that correlates with histologic synovitis. Arthroscopy is useful for assessment of stifle synovitis in client-owned dogs, and could be used in longitudinal clinical trials to monitor synovial responses to disease-modifying therapy.

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Role of Synovial Immune Responses in Stifle Synovitis

Muir¹

2010

Advances in the Canine Cranial Cruciate Ligament

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Localization of Cathepsin K and Tartrate‐Resistant Acid Phosphatase in Synovium and Cranial Cruciate Ligament in Dogs with Cruciate Disease

Abstract: Production of matrix-degrading enzymes by the synovium may induce progressive pathologic rupture of the CCL. Therefore, these collagenolytic pathways may offer a novel target for medical therapy of joint inflammation in canine patients with cruciate disease.

Cited by 52 publications

References 39 publications

Tartrate-resistant acid phosphatase, matrix metalloproteinase-2 and tissue inhibitor metalloproteinase-2 in early stages of canine osteoarthritis

Tartrate-resistant acid phosphatase, matrix metalloproteinase-2 and tissue inhibitor metalloproteinase-2 in early stages of canine osteoarthritis

Arthroscopic Assessment of Stifle Synovitis in Dogs with Cranial Cruciate Ligament Rupture

Role of Synovial Immune Responses in Stifle Synovitis

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