Localization of beta 1- and beta 2-adrenergic receptors in rat kidney by autoradiography.

Healy, Dennis P.; Münzel, Peter A.; Insel, Paul A.

doi:10.1161/01.res.57.2.278

Cited by 33 publications

(8 citation statements)

References 22 publications

(24 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Summers et al demonstrated that those receptors distributing in the distal nephron are mainly of the/?1 subtype, while both/I1 and 82 subtypes of receptors are present in glomeruli [8], data largely in agreement with our present results except for the lack of proximal 82 receptors. In contrast Healy et al have reported that those receptors distributing in glome~li are mainly of the pl subtype whilst the /I2 subtype of receptors are distributed in medullary tubules [22]. Although the reasons for the conflicting data reported in the literature, as well as some disagreements of our present results with others, may not be identified unequivocally, the problem of cross-reactivity of pharmacological agents should always be considered.…”

Section: Rt-pcr Of Intronless Genes Has Been Reported Bycontrasting

confidence: 96%

“…and Teitelbaum et al [15] who showed by using atenolol that /?-adrenergic receptors of subtype other than /I1 are present in cultured IMCD cells [15]), and from those studies it is not clear which subtype of receptors is invoived in most of the nephron segments. Using subtype-specific agents, two groups have reported the distribution of ,/31 and 82 subtypes of adrenergic receptors in the kidney by autoradiography [8,22], but their results are not totally in agreement with each other. Summers et al demonstrated that those receptors distributing in the distal nephron are mainly of the/?1 subtype, while both/I1 and 82 subtypes of receptors are present in glomeruli [8], data largely in agreement with our present results except for the lack of proximal 82 receptors.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Distribution of β2‐adrenergic receptor mRNA expression along the hamster nephron segments

et al. 1993

View full text Add to dashboard Cite

Distribution of 82-adrenergic receptor mRNA expression along the microdissected hamster nephron segments was examined by the reverse transcription-polymerase chain reaction (RT-PCR) technique. Conventional RT-PCR using a set of primers on separate exons could not be applied for the detection offi2-adrenergic receptor mRNA because of its intronless nature. We used the 'rapid amplification of cDNA ends' protocol [(1985) Proc. Natl. Acad. Sci. USA 85, 8998-90021 as a maneuver for RT-PCR of an intronless gene. Using this method, we successfully located hamster /?2-adrenergic receptor mRNA only in glomeruli and early proximal convoluted tubule along the nephron segments tested.

show abstract

Section: Rt-pcr Of Intronless Genes Has Been Reported Bycontrasting

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Distribution of β2‐adrenergic receptor mRNA expression along the hamster nephron segments

et al. 1993

View full text Add to dashboard Cite

show abstract

“…In contrast, collecting tubules present in outer medulla mainly contained P2-adrenoceptors. These localizations of renal P-adrenoceptors are in general agreement with other reports (Summers & Kuhar, 1983;Summers et al, 1985;Healy et al, 1985). Surgical denervation did not alter the density of P-adrenoceptor subtypes present at cortical and outer medullary locations, implying that these receptors are not associated with renal sympathetic nerves innervating these structures (Barajas & Powers, 1990).…”

Section: Autoradiographysupporting

confidence: 91%

Pharmacological characterization and anatomical localization of prejunctional β‐adrenoceptors in the rat kidney

Lakhlani

Amenta

Napoleone

et al. 1994

British J Pharmacology

View full text Add to dashboard Cite

1 The subtype and anatomical localization of P-adrenoceptors mediating facilitation of stimulusinduced overflow of noradrenaline ('prejunctional P-adrenoceptors') are not conclusively known to date.The present study was undertaken to characterize these receptors by use of pharmacological methods as well as to define their localization (prejunctional or postjunctional) with radio-ligand binding and autoradiography techniques combined with surgical denervation of the sympathetic innervation to the rat kidney.2 Exposure of the kidney to (-)-isoprenaline, the nonselective P-adrenoceptor agonist, resulted in a dose-dependent facilitation of stimulus-induced neurotransmitter overflow. This response was inhibited by propranolol, the f,-and P2-adrenoceptor antagonist, with a pA2 of 9.20 suggesting that the prejunctional P-adrenoceptors are not of the P3-subtype. 3 The rank order of potency and potency ratios of P-adrenoceptor agonists at renal prejunctional P-adrenoceptors (EC50 for agonist/EC50 for (-)-isoprenaline) were: (-)-isoprenaline (1) >procaterol (2) > salbutamol (3) >adrenaline (10) > (+)-isoprenaline (25). However, dobutamine, the Pf-adrenoceptor agonist, failed to enhance stimulus-induced overflow of noradrenaline. These results are indicative of the presence of P2-adrenoceptors as prejunctional ,-adrenoceptors. 4 Facilitation elicited by (-)-isoprenaline and procaterol, the selective P2-adrenoceptor agonist, was inhibited by ICI 118,551, the selective P2-adrenoceptor antagonist, with pKb values of 9.20 and 9.35, respectively at renal prejunctional P-adrenoceptors. Similarly, the pKb values of metoprolol, the selective fi-adrenoceptor antagonist, at renal prejunctional P-adrenoceptors were determined to be 6.25 and 6.18 against (-)-isoprenaline and procaterol, respectively. These results suggest the presence of a homogeneous population of P2-adrenoceptors as prejunctional P-adrenoceptors. 5 Radio-ligand binding analysis of renal P-adrenoceptors revealed the prevalence of the fi,-subtype as compared to the P2-subtype (63% vs 37%). However, surgical denervation of the rat kidney, resulting in more than 90% reduction in renal noradrenaline content, selectively reduced the P2-adrenoceptor population by 80%, implying the presence of P2-adrenoceptors on renal sympathetic nerve terminals.6 Autoradiographic analysis demonstrated the presence of P,-adrenoceptors on cortical structures such as glomeruli and tubules. fi2-Adrenoceptors were found to be present on tubules (minor population), collecting tubules in outer medulla and the adventitia and adventitial-medial border of intraparenchymal branches of the renal artery. Surgical denervation of the rat kidney resulted in the disappearance of P2-adrenoceptors associated with the intraparenchymal branches, without affecting the P-adrenoceptor populations at other sites. These results support the notion that the P2-subtype is present on renal sympathetic nerve terminals and demonstrate that these prejunctional P2-adrenoceptors are associated with the renal vasculature ...

show abstract

“…Renal βARs are involved in the modulation of both hemodynamics and electrolyte balance [167]. β 2 AR has been shown to regulate the expression of Na/Cl co-transporter expression, thereby participating in the development of salt sensitive hypertension [168,169].…”

Section: Introductionmentioning

confidence: 99%

Pinpointing beta adrenergic receptor in ageing pathophysiology: victim or executioner? Evidence from crime scenes

Santulli

Iaccarino

2013

Immun Ageing

View full text Add to dashboard Cite

G protein-coupled receptors (GPCRs) play a key role in cellular communication, allowing human cells to sense external cues or to talk each other through hormones or neurotransmitters. Research in this field has been recently awarded with the Nobel Prize in chemistry to Robert J. Lefkowitz and Brian K. Kobilka, for their pioneering work on beta adrenergic receptors (βARs), a prototype GPCR. Such receptors, and β2AR in particular, which is extensively distributed throughout the body, are involved in a number of pathophysiological processes. Moreover, a large amount of studies has demonstrated their participation in ageing process. Reciprocally, age-related changes in regulation of receptor responses have been observed in numerous tissues and include modifications of βAR responses. Impaired sympathetic nervous system function has been indeed evoked as at least a partial explanation for several modifications that occur with ageing. This article represents an updated presentation of the current knowledge in the field, summarizing in a systematic way the major findings of research on ageing in several organs and tissues (crime scenes) expressing βARs: heart, vessels, skeletal muscle, respiratory system, brain, immune system, pancreatic islets, liver, kidney and bone.

show abstract

Localization of beta 1- and beta 2-adrenergic receptors in rat kidney by autoradiography.

Cited by 33 publications

References 22 publications

Distribution of β2‐adrenergic receptor mRNA expression along the hamster nephron segments

Distribution of β2‐adrenergic receptor mRNA expression along the hamster nephron segments

Pharmacological characterization and anatomical localization of prejunctional β‐adrenoceptors in the rat kidney

Pinpointing beta adrenergic receptor in ageing pathophysiology: victim or executioner? Evidence from crime scenes

Contact Info

Product

Resources

About