Localization and hormonal regulation of endometrial matrix metalloproteinase-26 in the rhesus macaque

Almeida-Francia, Camila Contin Diniz de; Keator, Christopher S.; Mah, Kunie; Holden, Lindsay A.; Hergert, C.; Slayden, Ov D.

doi:10.1093/humrep/des086

Cited by 14 publications

(12 citation statements)

References 48 publications

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“…Quantitative real‐time PCRs (RT‐qPCR) were carried out on a 7500 Fast Real‐time PCR System (ABI; Applied Biosystems, Carlsbad, CA, USA). Real‐time PCRs were performed in duplicate on 2 μL of diluted (1:2) cDNA and compared against a 5‐point standard curve of pooled oviductal RNA . Table shows PCR primers and hydrolysis probes.…”

Section: Methodsmentioning

confidence: 99%

“…Samples for RNA isolation were frozen in liquid nitrogen and stored Table 2 shows PCR primers and hydrolysis probes. Target genes' expression was referenced to Ribosomal S10 RNA expression 21 and averaged to give a single level of expression.…”

Section: Rna Analysismentioning

confidence: 99%

“…Immunohistochemistry for OVGP1 was conducted on (6 μm) paraffin sections using a standardized technique 21 Immunohistochemistry for ESR1 and PGR was conducted on 7 μm cryosections as previously described. 18 Target-specific antibodies were anti-ESR1 (estrogen receptor 1) Neomarkers cat#Ms- Figure 4C).…”

Section: Immunohistochemistrymentioning

confidence: 99%

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Hormonal regulation of oviductal glycoprotein 1 (OVGP1; MUC9) in the rhesus macaque cervix

Slayden

Friason

Bond

et al. 2018

J of Medical Primatology

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Section: Methodsmentioning

confidence: 99%

Section: Rna Analysismentioning

confidence: 99%

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Hormonal regulation of oviductal glycoprotein 1 (OVGP1; MUC9) in the rhesus macaque cervix

Slayden

Friason

Bond

et al. 2018

J of Medical Primatology

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“…Immunohistochemistry was conducted using a standardized technique [12] at room temperature unless otherwise noted. Slides were deparaffinized in xylene, rehydrated stepwise in ethanol:H 2 O and then rinsed in deionized water.…”

Section: Methodsmentioning

confidence: 99%

Polidocanol induced tubal occlusion in nonhuman primates: immunohistochemical detection of collagen I-V

et al. 2016

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ObjectiveIntrauterine administration of polidocanol foam (PF) can create fallopian tube occlusion in nonhuman primates. The objective of this study was to determine if PF-induced tubal obstructions contain collagen in the extracellular matrix.Study designWe compared tissue samples of the intramural fallopian tube obtained from previous studies evaluating the effects of intrauterine infusion of 5% PF 2–12 weeks after treatment. Serial sections of the intramural portion of the fallopian tube obtained from representative treated (rhesus macaques, n=7; baboon, n=11) and untreated control (macaque, n=3; baboon, n=5) animals were stained with hematoxylin and eosin to identify tubal occlusion and by immunohistochemistry for collagens Col-I, Col-III and Col-IV. Descriptive results are summarized.ResultsControl animals exhibited histologically normal fallopian tubal epithelium with no staining for Col-1, light staining for Col-III and Col-V in the lamina propria and Col-IV distributed evenly in the extracellular matrix of the lamina propria. Treatment with PF resulted in acute tissue damage confined to the intramural tube; no epithelial damage or occlusion occurred in the tubal isthmus or ampulla. Blockade of the intramural tube demonstrated fibrosis with the epithelium replaced with extracellular matrix that stained strongly for Col-I, Col-III, Col-IV and Col-V. Col-II was undetectable.ConclusionTubal blockage induced by PF resulted in loss of normal epithelium and accumulation of collagens Col-I, Col-III, Col-IV and Col-V at the site of obstruction. The presence of dense collagen staining supports the hypothesis that PF infusion creates lasting tubal obstructions.ImplicationsThis study demonstrates that PF-induced tubal occlusion results in deposition of collagens suggesting the potential for a more lasting blockade. The structural nature of this occlusion supports the development of intrauterine administration of PF as a nonsurgical method of permanent contraception.

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“…3) is strongly increased in the glandular epithelium preceding the window of implantation. 55 Therefore, to understand the molecular and cellular pathways that are involved in secretory differentiation, it is essential to micro-dissect the endometrial functionalis zone from basalis zone as well as separate glandular, vascular, and stromal compartments for analysis. In a recent study, Okulicz's laboratory 56 combined laser capture microscopy with quantitative PCR analysis, as well as differential display analysis, to assess tissue-specific targets.…”

Section: Functional Genomicsmentioning

confidence: 99%

Cyclic Remodeling of the Nonhuman Primate Endometrium: A Model for Understanding Endometrial Receptivity

Slayden

2014

Semin Reprod Med

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Old World monkeys display physiological responses to steroid hormones that are similar to those of women. In this review, we describe cyclic morphological changes that take place within the uterus of Old World primates during the menstrual cycle. In primates, estrogen stimulates endometrial growth in the follicular phase of the menstrual cycle. Progesterone secreted in the luteal phase acts to induce secretory differentiation, which is required for successful embryo implantation. During the differentiation process, endometrial estrogen receptor-1 (ESR-1) is suppressed, and reduced staining for ESR-1 is a definitive marker of the onset of uterine receptivity. Downregulation of ESR-1 is topographically limited to the functionalis (upper) zones of the endometrium, the zones in which embryo implantation occurs, indicating that zone-specific factors play a role in the differentiation process. Future genomic and proteomic studies are expected to reveal additional markers for diagnosing endometrial receptivity. Due to the distinct zonal response of the endometrium to ovarian steroids, accurate histological characterization will remain necessary to interpret novel targets in the assessment of fertility.

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Localization and hormonal regulation of endometrial matrix metalloproteinase-26 in the rhesus macaque

Cited by 14 publications

References 48 publications

Hormonal regulation of oviductal glycoprotein 1 (OVGP1; MUC9) in the rhesus macaque cervix

Hormonal regulation of oviductal glycoprotein 1 (OVGP1; MUC9) in the rhesus macaque cervix

Polidocanol induced tubal occlusion in nonhuman primates: immunohistochemical detection of collagen I-V

Cyclic Remodeling of the Nonhuman Primate Endometrium: A Model for Understanding Endometrial Receptivity

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