Localization and Characterization of a Short Isoform of the Corticotropin-Releasing Factor Receptor Type 2α (CRF2α-tr) in the Rat Brain

Miyata, Ichiro; Shiota, Chiyo; Chaki, Shigeyuki; Okuyama, Shigeru; Inagami, Tadashi

doi:10.1006/bbrc.2000.4112

Cited by 31 publications

(26 citation statements)

References 24 publications

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“…These brain regions include the raphe nucleus (Lukkes et al, 2011), multiple nuclei of the hypothalamus and the amygdala (Blume et al, 2009; Campbell, Grove, & Smith, 2003; Sajdyk et al, 2004; Smialowska, Wieronska, & Wedzony, 2002), pituitary (Stanley et al, 2004), cortex and lateral septum (Miyata, Shiota, Chaki, Okuyama, & Inagami, 2001), bed nucleus of the stria terminalis and hippocampus (Van Pett et al, 2000). Moreover, CRF-R1 receptors colocalize with cholinergic, noradrenergic and DAergic neurons in many of these brain regions as well (Sauvage & Steckler, 2001).…”

Section: Some Neurochemical Neuropharmacological As Well As Neurmentioning

confidence: 99%

A Genetic Animal Model of Alcoholism for Screening Medications to Treat Addiction

Bell

Hauser

Rodd

et al. 2016

International Review of Neurobiology

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The purpose of this review is to present up-to-date pharmacological, genetic and behavioral findings from the alcohol-preferring P rat and summarize similar past work. Behaviorally, the focus will be on how the P rat meets criteria put forth for a valid animal model of alcoholism with a highlight on its use as an animal model of polysubstance abuse, including alcohol, nicotine and psychostimulants. Pharmacologically and genetically, the focus will be on the neurotransmitter and neuropeptide systems that have received the most attention: cholinergic, dopaminergic, GABAergic, glutamatergic, serotonergic, noradrenergic, corticotrophin releasing hormone, opioid, and neuropeptide Y. Herein we sought to place the P rat’s behavioral and neurochemical phenotypes, and to some extent its genotype, in the context of the clinical literature. After reviewing the findings thus far, this paper discusses future directions for expanding the use of this genetic animal model of alcoholism to identify molecular targets for treating drug addiction in general.

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Section: Some Neurochemical Neuropharmacological As Well As Neurmentioning

confidence: 99%

A Genetic Animal Model of Alcoholism for Screening Medications to Treat Addiction

Bell

Hauser

Rodd

et al. 2016

International Review of Neurobiology

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“…The tr-isoform includes three transmembrane domains and a fragment of the fourth transmembrane domain of fl CRF-R2. The remaining membrane loops, as well as, the C-terminus are absent in the tr-isoform (21)(22)(23). The molecular weight of the tr-isoform is approximately 22 kDa, that of the fl one is 65 kDa (17,18).…”

Section: Crf-r2a Genetics and Cerebellar Localizationmentioning

confidence: 97%

“…The precise role of the truncated receptor has not been defined yet. CRF-R2 tr does not bind UCN and savagine, hence the ligand of this receptor is exclusively CRF (21,23). Furthermore, the truncated isoform does not increase cAMP-level (Figure 3), suggesting that it uses different second messenger pathways and/or subserves a unique function (21).…”

Section: Functional Roles Of Crf-r2 Isoformsmentioning

confidence: 99%

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Localization and functional roles of corticotropin-releasing factor receptor type 2 in the cerebellum

2008

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The corticotropin-releasing factor (CRF) type 2 receptor has three splice variants alpha, beta, and gamma. In the rodent brain only CRFR2alpha is present. In the cerebellum, CRF-R2alpha has two different isoforms: a full-length form (fl) and truncated (tr). Both forms CRF-R2 have a unique cellular distribution. During postnatal cerebellar development, the expression patterns of tr and fl isoforms are changing. This suggests that, CRF and the related peptide urocortin (UCN) could play distinct roles in the immature and adult cerebellum, acting via different receptors subtypes. This review focuses on differences in the distribution of each isoform of CRF-R2 in view of their relationship to CRF and UCN release sites and their possible functional implications. Moreover, it includes novel findings of molecular pathways activating CRF-R2 isoforms through which CRF and UCN excert their specific actions.

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“…In contrast, CRF-R2a, is absent in the periphery, but has a major distribution in the CNS. Recently, a truncated isoform of CRF-2a has been described and is designated CRF-2a-tr (Miyata et al 1999a(Miyata et al , 2001Dautzenberg et al 2001); it also is located in the CNS. CRF-2a-tr contains the first three and a part of the fourth transmembrane domains of the fulllength type 2 CRF receptor.…”

Section: Crf Receptors In the Cerebellummentioning

confidence: 99%

Modulatory Role of Neuropeptides in the Cerebellum

Bishop¹,

King²

2013

Handbook of the Cerebellum and Cerebellar Disorders

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The existence of neuropeptides in the central nervous system has been known for almost 40 years. Within the cerebellum approximately 22 neuropeptides have been identified. However, the functional role(s) for many of the different peptides in the cerebellum is largely unknown as their distribution in the cerebellum is not uniform and varies between species. Further, to date sufficient physiological analysis has not been carried out to determine the potential importance of cerebellar peptides. It has been proposed that peptides slowly modulate cerebellar circuits by acting through G protein-coupled receptors in contrast to the fast action of amino acid neurotransmitters that bind to ionotropic receptors. This effect, in turn, would influence the state of an entire neuronal circuit rather than a single neuron. This chapter will be focused on two members of the corticotropin-releasing factor (CRF) family of peptides, namely, corticotropin-releasing factor and urocortin because their distribution has been described in multiple species and their function has been investigated to a greater extent than any of the other peptides found in the cerebellum. A review of the distribution of these peptides as well as a discussion of their cognate receptors is included. Data on the functional role of both peptides is presented. It is clear that complex interactions take place between these peptides and other transmitters in the cerebellum. The physiological data suggest that CRF is essential for normal cerebellar-mediated motor performance. There is less data on the physiological role of urocortin (UCN) in the cerebellum. To date, it has been shown that UCN increases the spontaneous firing rate

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Localization and Characterization of a Short Isoform of the Corticotropin-Releasing Factor Receptor Type 2α (CRF2α-tr) in the Rat Brain

Cited by 31 publications

References 24 publications

A Genetic Animal Model of Alcoholism for Screening Medications to Treat Addiction

A Genetic Animal Model of Alcoholism for Screening Medications to Treat Addiction

Localization and functional roles of corticotropin-releasing factor receptor type 2 in the cerebellum

Modulatory Role of Neuropeptides in the Cerebellum

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