Local parasite pressures and host genotype modulate epigenetic diversity in a mixed‐mating fish

Berbel‐Filho, Waldir M.; Leániz, Carlos García de; Morán, Paloma; Cable, Joanne; Lima, Sérgio M. Q.; Consuegra, Sofía

doi:10.1002/ece3.5426

Cited by 17 publications

(25 citation statements)

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“…Kryptolebias ocellatus has been historically bred in aquaria, and while behavioural observations indicate it reproduces via outcrossing (Seegers, 1984), its populations are composed of males and simultaneous hermaphrodites (Costa et al, 2010), leaving open the possibility that this species may also undergoes self-fertilisation. However, while the genetic analysis of two populations in this species found no evidence for selfing (Tatarenkov et al, 2009), the possibility of self-fertilisation at a broader geographical scale cannot be ruled out, as rates of selfing and outcrossing are known to vary geographically in the mixed-mating Kryptolebias species (Berbel-Filho et al, 2019;Tatarenkov et al 2011). In the northernmost part of its distribution (Guanabara and Sepetiba Bays, 22° S), K. ocellatus is often syntopic (i.e.…”

Section: Introductionmentioning

confidence: 99%

More than meets the eye: syntopic and morphologically similar mangrove killifish species show different mating systems and patterns of genetic structure along the Brazilian coast

et al. 2020

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Different mating systems can strongly affect the extent of genetic diversity and population structure among species. Given the increased effects of genetic drift on reduced population size, theory predicts that species undergoing selffertilization should have greater population structure than outcrossed species, however demographic dynamics may affect this scenario. The mangrove killifish clade is composed of the two only known examples of self-fertilising species among vertebrates (Kryptolebias marmoratus and K. hermaphroditus). A third species in this clade, K. ocellatus, inhabits mangrove forests in southeast Brazil, however its mating system and patterns of genetic structure have been rarely explored. Here, we examined the genetic structure and phylogeographic patterns of K. ocellatus along its distribution, using mitochondrial DNA and microsatellites to compare its patterns of genetic structure with the predominantly selfing and often syntopic, K. hermaphroditus. Our results indicate that K. ocellatus reproduces mainly by outcrossing across much of its known range, with no current evidence of selfing, despite being an androdioecious species. Our results also reveal a stronger population subdivision in K. ocellatus compared to K. hermaphroditus, contrary to the theoretical predictions based on reproductive biology of the two species Our findings indicate that, although morphologically similar, K. ocellatus and K. hermaphroditus had remarkably different evolutionary histories when colonising the same mangrove areas in south-eastern Brazil, with other factors (e. g. time of colonisation, dispersal/establishment capacity) having more profound effects on the current population structuring of those species than differences in mating systems.

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Section: Introductionmentioning

confidence: 99%

More than meets the eye: syntopic and morphologically similar mangrove killifish species show different mating systems and patterns of genetic structure along the Brazilian coast

et al. 2020

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Section: Introductionmentioning

confidence: 82%

“…In the northernmost part of its distribution (Guanabara and Sepetiba Bays, 22° S), K. ocellatus is often syntopic (i.e. coexisting at the same habitat at the same time) with K. hermaphroditus , a species composed mostly of self-fertilising hermaphrodites and very rare males (Berbel-Filho et al, 2016;, resulting in occasional outcrossing but at very low frequencies (Berbel-Filho et al, 2019;Tatarenkov et al, 2017). Extremely low levels of genetic diversity in K. hermaphroditus, especially at the southernmost edge of its distribution (where it is syntopic with K. ocellatus), suggest relatively recent dispersal and colonisation of this species in south-eastern Brazil (Tatarenkov et al, 2009;.…”

Section: Introductionmentioning

confidence: 99%

More than meets the eye: syntopic and morphologically similar mangrove killifish species show different mating systems and patterns of genetic structure along the Brazilian coast

Berbel‐Filho

Tatarenkov

Espírito‐Santo

et al. 2020

Preprint

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AbstractDifferent mating systems can strongly affect the extent of genetic diversity and population structure among species. Given the increased effects of genetic drift on reduced population size, theory predicts that species undergoing self-fertilization should have greater population structure than outcrossed species, however demographic dynamics may affect this scenario. The mangrove killifish clade is composed of the two only known examples of self-fertilising species among vertebrates (Kryptolebias marmoratus and K. hermaphroditus). A third species in this clade, K. ocellatus, inhabits mangrove forests in southeast Brazil, however its mating system and patterns of genetic structure have been rarely explored. Here, we examined the genetic structure and phylogeographic patterns of K. ocellatus along its distribution, using mitochondrial DNA and microsatellites to compare its patterns of genetic structure with the predominantly selfing and often syntopic, K. hermaphroditus. Our results indicate that K. ocellatus reproduces mainly by outcrossing across much of its known range, with no current evidence of selfing, despite being an androdioecious species. Our results also reveal a stronger population subdivision in K. ocellatus compared to K. hermaphroditus, contrary to the theoretical predictions based on reproductive biology of the two species Our findings indicate that, although morphologically similar, K. ocellatus and K. hermaphroditus had remarkably different evolutionary histories when colonising the same mangrove areas in south-eastern Brazil, with other factors (e. g. time of colonisation, dispersal/establishment capacity) having more profound effects on the current population structuring of those species than differences in mating systems.

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“…Recent studies described the DNA methylation reprogramming event during embryogenesis , as well as the time course expression of enzymes involved in epigenetics during its development (Fellous, Earley, & Silvestre, 2019a;Fellous, Earley, & Silvestre, 2019b). Another study has shown that parasite load can modify DNA methylation (Berbel-Filho et al, 2019a), while these authors also found a higher differentiation of the methylation landscape between different genotypes than between environments (Berbel-Filho et al, 2019b).…”

Section: Introductionmentioning

confidence: 99%

Genome-wide DNA methylation of the liver reveals delayed effects of early-life exposure to 17-α-ethinylestradiol in the self-fertilizing mangrove rivulus

Voisin

Ulloa

Stockwell

et al. 2020

Preprint

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Organisms exposed to endocrine disruptors in early life can show altered phenotype later in adulthood. Although the mechanisms underlying these long-term effects remain poorly understood, an increasing body of evidence points toward the potential role of epigenetic processes. In the present study, we exposed hatchlings of an isogenic lineage of the self-fertilizing fish mangrove rivulus for 28 days to 4 and 120 ng/L of 17-α-ethinylestradiol. After a recovery period of 140 days, reduced representation bisulfite sequencing (RRBS) was performed on the liver in order to assess the hepatic genome-wide methylation landscape. Across all treatment comparisons, a total of 146 differentially methylated fragments (DMFs) were reported, mostly for the group exposed to 4 ng/L, suggesting a non-monotonic effect of EE2 exposure. Gene ontology analysis revealed networks involved in lipid metabolism, cellular processes, connective tissue function, molecular transport and inflammation. The highest effect was reported for nipped-B-like protein B (NIPBL) promoter region after exposure to 4 ng/L EE2 (+ 21.9%), suggesting that NIPBL could be an important regulator for long-term effects of EE2. Our results also suggest a significant role of DNA methylation in intergenic regions and potentially in transposable elements. These results support the ability of early exposure to endocrine disruptors of inducing epigenetic alterations during adulthood, providing plausible mechanistic explanations for long-term phenotypic alteration. Additionally, this work demonstrates the usefulness of isogenic lineages of the self-fertilizing mangrove rivulus to better understand the biological significance of long-term alterations of DNA methylation by diminishing the confounding factor of genetic variability.

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Local parasite pressures and host genotype modulate epigenetic diversity in a mixed‐mating fish

Cited by 17 publications

References 97 publications

More than meets the eye: syntopic and morphologically similar mangrove killifish species show different mating systems and patterns of genetic structure along the Brazilian coast

More than meets the eye: syntopic and morphologically similar mangrove killifish species show different mating systems and patterns of genetic structure along the Brazilian coast

More than meets the eye: syntopic and morphologically similar mangrove killifish species show different mating systems and patterns of genetic structure along the Brazilian coast

Genome-wide DNA methylation of the liver reveals delayed effects of early-life exposure to 17-α-ethinylestradiol in the self-fertilizing mangrove rivulus

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