Local delivery of reporter gene to the cochlea does not spread to brain tissue in an animal model

The spread of adenoviral vectors to central nervous system through pathway of cochlea in mimetic aging and young rats

“…Our study shows that viral vectors can be detected by TEM and IF with an anti-GFP antibody, whereas the viral vectors in Stöver's study could be detected by PCR in the CNS. 32 By contrast, Duan and Mi 31 administered a viral suspension at a lower volume (1 μl) and a lower titer (10 5 pfu ml − 1 ) and presented a negative reporter gene expression. Lalwani et al 29 reported that with a low titer of 10 6 viral particles per milliliter viral suspension and with a large total volume of 100 μl injected at a slow speed, the virus was detected in the brain by PCR.…”

“…32 In contrast, some researchers have reported that viral vectors do not disseminate into the surrounding brain tissue upon a local injection of lentiviral vectors into rat scala tympani. 31 In addition, none of these data report the existence of viral vectors in the cerebrum. In the present study, viral vectors could spread to the brainstem and cerebrum as demonstrated by the detection of GFP expression from the AdV reporter gene with IF and the observation of viral particles with TEM.…”

“…In the past, a few studies have attempted to detect the viral vectors in the CNS after an inoculation in the cochlea; some have concluded that the viral vectors could be delivered into the brain via the cochlea, 29,30 whereas other researchers came to the opposite conclusion. 31,32 Furthermore, few functional studies have reported on viral vectors carrying a transgene into the CNS after administration via the cochlea. Moreover, no literature is available at present concerning the different efficiencies of transfection between young and aged individuals.…”