Local Antibiotic Delivery with Bovine Cancellous Chips

Lewis, Christine S.; Katz, Jordan; Baker, Maribel I.; Supronowicz, Peter; Gill, Elise; Cobb, Ronald R.

doi:10.1177/0885328210375729

Cited by 20 publications

(25 citation statements)

References 51 publications

(37 reference statements)

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“…[40] Figure 3(c) shows a simple circuit with sufficient fitting results, which is used very often. [4,12,26,29,32,36,[42][43][44][45][46][47][48] As a rule this equivalent circuit is used for a metal in contact with an electrolyte and it consists of the resistance of the solution R sol , a capacitance and the resistance R 1 , which can be a CTR [4,12,32] or the resistance of the passive layer. [32] The CTR represents the limited charge transfer through the interface and the electric double-layer capacitance.…”

Section: Different Equivalent Circuitsmentioning

confidence: 99%

“…[29] Lewis models the equivalent circuit with the capacitance of the surface oxide film of Ti6Al4V and R 1 being the sum of the CTR and the pore resistance with a possible electrolyte penetration into the pores of the surface. [43] A CPE is used instead of the capacitance in Figure 3(c) owing to an inhomogeneous coverage of a CoCrMb alloy and of different titanium alloy surfaces in contact with serum proteins and oxides. The CPE includes film and double layer capacitance in series.…”

Section: Different Equivalent Circuitsmentioning

confidence: 99%

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Biomaterial Interface Investigated by Electrochemical Impedance Spectroscopy

2008

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Electrochemical impedance spectroscopyElectrochemical impedance spectroscopy is an easy applicable sensitive technique. It is used to get information about the adsorption behaviour and the interactions of biomolecules with the electrode surface in a short measurement duration. Electrochemical processes can be modelled by an equivalent circuit and various parameters reflecting certain properties of the interface can be determined. The electrochemical properties of the interface are influenced by proteins and cells adsorbing at the surface. Different modified surfaces can be analyzed before, during, and after cell contact, respectively. Applying bias potentials also changes the behaviour of attaching cells and proteins. Changes in the medium composition have an influence on the electrochemically detectable surface properties.Biosensors are developed consecutively to detect biomolecules. Enzymes, lipids and membranes can be analyzed and the adsorption behaviour of DNA and antigens at modified electrode surfaces can be determined. Different technologies using microchips were constructed to allow a fast screening of electrochemical properties and discrimination of biological molecules or cells. ApplicationElectrochemical impedance spectroscopy serves not only for determination of the capacity of new battery materials or corrosion behaviour and long term stability of diverse alloys and oxide layers, it can also be used to gain information about biomolecules. The adsorption behaviour of cells [1][2][3] and proteins, [4] the amount of adsorbed proteins, [4][5][6][7] surface charge densities [4][5][6] and adsorption coefficients [5] can be determined. Interactions of implant surfaces with biological devices, changes in the polarizability of functional groups, the transport of ions or dipoles through polymer layers and mineralization processes, for instance of bone tissue can be assessed. The change of the Gibbs free energy and the entropy due to protein binding to the surface can be calculated [4,6,8] and the affinity of biomolecules to the surface can be estimated. [4] Electrochemical techniques are introduced as biosensors. Antigens, ssDNA or enzymes are immobilized at the surface and according antibodies, DNA and distinct proteins can be ADVANCED ENGINEERING MATERIALS 2008, 10, No. 10

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Section: Different Equivalent Circuitsmentioning

confidence: 99%

Section: Different Equivalent Circuitsmentioning

confidence: 99%

Biomaterial Interface Investigated by Electrochemical Impedance Spectroscopy

2008

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“…To prevent and treat bacterial infections and related infectious diseases, antibiotics can be administered systemically and locally on the surface of bone grafts [1,3,5]. It is highly important that the ingrowth and ossification process is not inhibited by the presence of such antibiotics.…”

Section: Discussionmentioning

confidence: 99%

“…Reconstruction of skeletal defects after trauma or disease [1][2][3], as well as repairing damage caused during surgery, has been a challenging problem in orthopaedics. The use of bone grafts quickly became a standard solution, since bone serves as a biologically active filling material and grows perfectly into spaces where a lack of material provides such a niche.…”

Section: Introductionmentioning

confidence: 99%

Effect of storage temperature and antibiotic impregnation on the quantity of bone morphogenetic protein seven in human bone grafts

Wurm

Nogler

Ammann

et al. 2014

International Orthopaedics (SICOT)

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Purpose The aim of this study was to quantify the amount of bone morphogenic protein 7 (BMP-7) in bone samples in different storage and treatment conditions used in bone banks and thereby evaluate the benefit of this test as a routine measure before bone grafting. Methods Fresh as well as frozen bone chips, each with and without antibiotic impregnation, were screened for their BMP-7 content. Human bone chips were produced from femoral heads of two female donors who had undergone total hip replacement surgery. The amount of BMP-7 was detected using a commercially available enzyme-linked immunosorbent assay (ELISA) test. Results There were no significant differences between groups in samples obtained from the first femoral head. Bone-chip samples derived from the second femoral head showed significant differences between groups. The actual amount of these differences was small and most likely biologically irrelevant. It is important to note that there was a significant difference between groups when comparing both femoral heads, reflecting donor-to-donor variability. Conclusion ELISA testing for BMP-7 as a qualitative measurement of bone grafts should be considered a routine quality-control test for bone banks.

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“…The release rate begins to plateau and all three concentrations begin eluting statistically similar quantities from day five out to day fourteen (P [ 0.10). The quantity of gentamicin eluting from each graft remained above the minimum effective concentration of 1.56 lg/mL for at least 13 days (Lewis et al 2010). …”

Section: Release Kineticsmentioning

confidence: 96%

Local antibiotic delivery with demineralized bone matrix

et al. 2011

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A method of care for these infected nonunions is prolonged intravenous systemic antibiotic treatment and implantation of methyl methacrylate antibiotic carrier beads to delivery high local doses of antibiotics. This method requires a second surgery to remove the beads once the infection has cleared. Recent studies have investigated the use of biodegradable materials that have been impregnated with antibiotics as tools to treat bone infections. In the present study, human demineralized bone matrix (DBM) was investigated for its ability to be loaded with an antibiotic. The data presented herein demonstrates that this osteoinductive and biodegradable material can be loaded with gentamicin and release clinically relevant levels of the drug for at least 13 days in vitro. This study also demonstrates that the antibiotic loaded onto the graft has no adverse effects on the osteoinductive nature of the DBM as measured in vitro and in vivo. This bone void filler may represent a promising option for local antibiotic delivery in orthopedic applications.

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Local Antibiotic Delivery with Bovine Cancellous Chips

Cited by 20 publications

References 51 publications

Biomaterial Interface Investigated by Electrochemical Impedance Spectroscopy

Biomaterial Interface Investigated by Electrochemical Impedance Spectroscopy

Effect of storage temperature and antibiotic impregnation on the quantity of bone morphogenetic protein seven in human bone grafts

Local antibiotic delivery with demineralized bone matrix

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