Loa loa and Onchocerca ochengi miRNAs detected in host circulation

Tritten, Lucienne; O’Neill, Maeghan; Nutting, Chuck; Wanji, Samuel; Njouendoui, Abdel; Fombad, Fanny Fri; Kengne-Ouaffo, Jonas; Mackenzie, Charles D.; Geary, Timothy G.

doi:10.1016/j.molbiopara.2014.11.001

Cited by 38 publications

(31 citation statements)

References 19 publications

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“…We further investigated the mature novel miRNA designated ‘chr26_14097’ with miRDeep2 in this study. Chr26_14097 has been detected in different bovine specimens in previous studies, including in milk‐isolated monocytes from cows infected in vivo with S. uberis via the teat canal ; alveolar macrophages induced in response to Mycobacterium bovis infection ; plasma of a cow ( Bos indicus ) infected with Onchocerca ochengi (a filarial parasite of cattle) ; and the whole blood and gut tissues of calves (30 min after delivery, and 7, 21, and 42 days old) , suggesting that chr26_14097 is present in the cells of the immune system and may regulate immune functions. According to our sequencing data, chr26_14097 was significantly upregulated in the CMT+ group, which was confirmed with digital PCR.…”

Section: Discussionmentioning

confidence: 90%

Bovine milk transcriptome analysis reveals microRNAs and RNU2 involved in mastitis

Lai

Lai²,

Rahman

et al. 2019

The FEBS Journal

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Mastitis is a common inflammatory infectious disease in dairy cows. To understand the microRNA (miRNA) expression profile changes during bovine mastitis, we undertook a genome-wide miRNA study of normal milk and milk that tested positive on the California mastitis test for bovine mastitis (CMT+). Twenty-five miRNAs were differentially expressed (23 miRNAs upregulated and two downregulated) during bovine mastitis relative to their expression in normal milk. Upregulated mature miR-1246 probably derived from a U2 small nuclear RNA rather than an miR-1246 precursor. The significantly upregulated miRNA precursors and RNU2 were significantly enriched on bovine chromosome 19, which is homologous to human chromosome 17. A gene ontology analysis of the putative mRNA targets of the significantly upregulated miRNAs showed that these miRNAs were involved in binding target mRNA transcripts and regulating target gene expression, and a Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that the upregulated miRNAs were predominantly related to cancer and immune system pathways. Three novel miRNAs were associated with bovine mastitis and were relatively highly expressed in milk. We confirmed that one of the novel mastitis-related miRNAs was significantly upregulated using a digital PCR system. The differentially expressed miR-NAs were involved in human cancers, infections, and immune-related diseases. The genome-wide analysis of miRNA profiles in this study provides insight into bovine mastitis and inflammatory diseases. DatabasesThe miRNAseq generated for this study can be found in the Sequence Read Archive (SRA) under BioProject Number PRJNA421075 and SRA Study Number SRP126134 (https://www. ncbi.nlm.nih.gov/bioproject/PRJNA421075).

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Section: Discussionmentioning

confidence: 90%

Bovine milk transcriptome analysis reveals microRNAs and RNU2 involved in mastitis

Lai

Lai²,

Rahman

et al. 2019

The FEBS Journal

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“…Another approach that has shown promise is the use of metabolome analysis of serum or urine samples from infected individuals, which has led to the identification of urinary N -acetyltyramine- O ,β-glucuronide (NATOG) as a unique biomarker for O. volvulus infection [19–21]. More recent work has focused on the detection of parasitic microRNAs in the blood of infected individuals, but the low levels of these biomarkers may pose a real challenge to be useful as a diagnostic marker [22–24]. …”

Section: Introductionmentioning

confidence: 99%

An isothermal DNA amplification method for detection of Onchocerca volvulus infection in skin biopsies

et al. 2016

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BackgroundDiagnostic procedures for the diagnosis of infection with the nematode parasite Onchocerca volvulus are currently based on the microscopic detection of microfilariae in skin biopsies. Alternative approaches based on amplification of parasitic DNA in these skin biopsies are currently being explored. Mostly this is based on the detection of the O-150 repeat sequence using PCR based techniques.MethodsAn isothermal, loop-mediated amplification method has been designed using the mitochondrial O. volvulus cox1 gene as a target.ResultsAnalysis of dilution series of synthetic DNA containing the targeted sequence show a non-linear dose-response curve, as is usually the case for isothermal amplification methods. Evaluation of cross-reactivity with the heterologous sequence from the closely related parasites Wuchereria bancrofti, Loa loa and Brugia malayi demonstrated strong specificity, as none of these sequences was amplified. The assay however amplified both O. volvulus and O. ochengi DNA, but with a different melting point that can be used to discriminate between the species. Evaluation of this assay in a set of skin snip biopsies collected in an endemic area in Ghana showed a high correlation with O-150 qPCR and also demonstrated a similar sensitivity. Compared to qPCR, LAMP had a sensitivity of 88.2% and a specificity of 99.2%.ConclusionsWe have developed a sensitive and specific loop-mediated amplification method for detection of O. volvulus DNA in skin biopsies that is capable of providing results within 30 min.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-016-1913-7) contains supplementary material, which is available to authorized users.

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“…Circulating miRNAs released by the intestinal nematode Heligmosomoides polygyrus [83] and filarial parasite Litomosoides sigmodontis [83] were found in sera from infected mice. More recently, circulating miRNAs have been described from other filarial parasites including O. volvulus, Dirofilaria immitis, Brugia pahangi, L. loa, and O. ochengi [81,84]. These studies set the stage for a future in RNA-based detection for filarial diagnostics.…”

Section: Isothermal Amplification Methodsmentioning

confidence: 98%

“…Plasma miRNAs are thought to be particularly stable because they are often encapsulated in small lipoprotein vesicles such as exosomes or in association with specific proteins [80]. Studies in nematodes have indicated that although some miRNAs are widely conserved, others are nematode-specific and unique to each species [81]. Parasite-derived miRNAs have been proposed as novel markers of active Schistosoma mansoni infection in mice and humans [82].…”

Section: Isothermal Amplification Methodsmentioning

confidence: 99%