LncRNA SAMMSON negatively regulates miR-9-3p in hepatocellular carcinoma cells and has prognostic values

Yang, Shouzhang; Cai, Huajie; Hu, Bingren; Tu, Jinfu

doi:10.1042/bsr20190615

Cited by 20 publications

(15 citation statements)

References 24 publications

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“…miR-9-3p has been confirmed as a tumor suppressor in a variety of different types of cancer, such as glioma ( 47 ), nasopharyngeal carcinoma ( 48 ) and bladder cancer ( 49 ). Of interest, in HCC cells, Yang et al ( 50 ) demonstrated that miR-9-3p overexpression reduced the migration and invasion rates of HCC in vitro . Tang et al ( 51 ) demonstrated that miR-9-3p, a functional biomarker, inhibited cell proliferation by suppressing human fibroblast growth factor-5 expression in HCC.…”

Section: Discussionmentioning

confidence: 99%

Long non‑coding RNA DUXAP8 promotes tumorigenesis by regulating IGF1R via miR‑9‑3p in hepatocellular carcinoma

et al. 2021

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Hepatocellular carcinoma (HCC) is the third leading cause of cancer-related death worldwide with a low 5-year survival rate. Long non-coding RNA (lncRNA) double homeobox A pseudogene 8 (DUXAP8) is an oncogene and a potential biomarker in various tumors, such as ovarian, colorectal and non-small-cell lung cancer. However, the function and molecular mechanism underlying DUXAP8 in HCC progression is not completely understood. The expression of DUXAP8, microRNA (miR)-9-3p and insulin-like growth factor 1 receptor (IGF1R) in HCC tissues and cells was detected via reverse transcription-quantitative PCR. The expression levels of IGF1R and epithelial-mesenchymal transition-associated proteins (Snail, Slug, E-cadherin, N-cadherin and vimentin) were assessed via western blotting. The effects of DUXAP8, miR-9-3p and IGF1R on proliferation, migration and invasion were examined by conducting Cell Counting Kit-8 and Transwell assays, respectively. The interaction between miR-9-3p and DUXAP8 or IGF1R was predicted using StarBase or TargetScan, and further assessed using dual luciferase reporter and RNA immunoprecipitation assays. DUXAP8 and IGF1R were upregulated and miR-9-3p was downregulated in HCC tissues and cells compared with adjacent healthy tissues and a normal liver cell line, respectively. miR-9-3p overexpression decreased the protein expression level of IGF1R, and miR-9-3p knockdown enhanced the protein expression level of IGF1R in HCC cells compared with the corresponding control groups. Moreover, compared with the corresponding control groups, DUXAP8 knockdown and miR-9-3p overexpression increased E-cadherin protein expression levels, and decreased Snail, Slug, N-cadherin and vimentin protein expression levels. However, miR-9-3p inhibitor and IGF1R overexpression reversed DUXAP8 knockdown-and miR-9-3p overexpression-induced effects, respectively. In addition, compared with the corresponding control groups, DUXAP8 knockdown and miR-9-3p overexpression suppressed proliferation, migration and invasion, which was reversed by miR-9-3p inhibitor and IGF1R overexpression, respectively. Moreover, miR-9-3p as the target of DUXAP8 and IGF1R as the target of miR-9-3p were verified in HCC cells. lncRNA DUXAP8 contributed to HCC tumorigenesis via the miR-9-3p/IGF1R axis, providing a novel therapeutic approach for HCC diagnosis and treatment.

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Section: Discussionmentioning

confidence: 99%

Long non‑coding RNA DUXAP8 promotes tumorigenesis by regulating IGF1R via miR‑9‑3p in hepatocellular carcinoma

et al. 2021

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“…evidence has indicated that over-expression of miR-9-5p could enhance cell migration and invasion while miR-9-3p could inhibit cell migration an invasion in HCC cells [20,31]. However, in this study, we revealed that miR-9-5p was a suppressor, we had examined the level of miR-9 and found that it was lower in cancer tissue than that in normal tissue and up-regulation of miR-9 could enhance sensitivity to cisplatin in HCC cells.…”

Section: Accumulatedmentioning

confidence: 99%

Overexpression of microRNA-9 enhances cisplatin sensitivity in hepatocellular carcinoma by regulating EIF5A2-mediated epithelial-mesenchymal transition

Bao¹,

Zhang²,

Lu³

et al. 2020

Int. J. Biol. Sci.

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We investigated the role of microRNA (miR)-9 in modulating chemoresistance in hepatocellular carcinoma (HCC) cells. MiR-9 was overexpressed or knocked down in HCC cell lines. Cell viability, cell proliferation, the expression of EIF5A2 and the epithelial-mesenchymal transition (EMT)-related proteins were examined. HCC cells overexpressing miR-9 were more sensitive to cisplatin; miR-9 knockdown yielded the opposite result. The in vivo nude mouse HCC xenograft tumors yielded the same results. EIF5A2 was identified as a potential target of miR-9, where miR-9 regulated EIF5A2 expression at mRNA and protein level. EIF5A2 knockdown reversed miR-9 inhibition-mediated cisplatin resistance. Altering miR-9 and EIF5A2 expression changed E-cadherin and vimentin expression. Furthermore, EIF5A2 mediated miR-9 EMT pathway regulation, indicating that miR-9 can enhance cisplatin sensitivity by targeting EIF5A2 and inhibiting the EMT pathway. Targeting miR-9 may be useful for overcoming drug resistance in HCC.

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“…It has been reported that aberrantly expressed long non-coding RNAs (lncRNAs) were aberrantly expression in the occurrence and development of HCC, which are identi ed as the oncogenes or tumor suppressors. For example, SAMMSON expression level was upregulated in HCC tissues, and patients with high SAMMSON level had signi cantly lower overall rate within ve years after admission (6).…”

Section: Introductionmentioning

confidence: 99%

Silencing LncRNA SCAMP1 inhibits cell proliferation, induces G0/G1 arrest and apoptosis in hepatocellular carcinoma

Ma¹,

Sun²,

Li³

et al. 2021

Preprint

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Background: Long noncoding RNA secretory carrier membrane protein 1 (SCAMP1) has been recently reported to be an oncogene in glioma and ovarian cancer. However, little is known about the clinical significance and functional role of SCAMP1 in hepatocellular carcinoma (HCC).Methods: The expression of SCAMP1 was determined in tissue samples and cell lines using quantitative reverse transcription PCR (qRT-PCR). The clinical significance of SCAMP1 in HCC was evaluated using chi-squared test, Kaplan-Meier survival, as well as univariate and multivariate analysis. Cell proliferation, cell cycle distribution and apoptosis were evaluated using CCK-8 assay, colony formation assay, Flow cytometry analysis. Related protein expression levels were measured by western blot analysis.Results: We found SCAMP1 expression levels were remarkably up-regulated in HCC tissues compared to that in matched adjacent tissues. Increased SCAMP1 expression was significantly correlated with large tumor size, advanced TNM stage and poor survival prognosis in HCC. Knockdown of SCAMP1 significantly inhibited cell proliferation, induced G0/G1 phase arrest and apoptosis in HepG2 and SNU-182 cells. More importantly, knockdown of SCAMP1 downregulated the protein levels of CDK4, Cyclin D1 and Bcl-2, while upregulated the protein levels of p21, p53 and Bax.Conclusions: In summary, our study clarified a positive correlation of the SCAMP1 levels with the HCC cell proliferation.

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LncRNA SAMMSON negatively regulates miR-9-3p in hepatocellular carcinoma cells and has prognostic values

Cited by 20 publications

References 24 publications

Long non‑coding RNA DUXAP8 promotes tumorigenesis by regulating IGF1R via miR‑9‑3p in hepatocellular carcinoma

Long non‑coding RNA DUXAP8 promotes tumorigenesis by regulating IGF1R via miR‑9‑3p in hepatocellular carcinoma

Overexpression of microRNA-9 enhances cisplatin sensitivity in hepatocellular carcinoma by regulating EIF5A2-mediated epithelial-mesenchymal transition

Silencing LncRNA SCAMP1 inhibits cell proliferation, induces G0/G1 arrest and apoptosis in hepatocellular carcinoma

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